Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Abstract

It is supposed that plant functional foods, rich in phytochemicals, may potentially have preventive effects in carcinogenesis. In this study, the anticancer effects of cloves in the in vivo and in vitro mammary carcinoma model were assessed. Dried flower buds of cloves (CLOs) were used at two concentrations of 0.1% and 1% through diet during 13 weeks after the application of chemocarcinogen. After autopsy, histopathological and immunohistochemical analyses of rat mammary carcinomas were performed. Moreover, in vitro evaluation using MCF‐7 cells was carried out. Dietary administered CLO caused the dose‐dependent decrease in tumour frequency by 47.5% and 58.5% when compared to control. Analysis of carcinoma cells in animals showed bcl‐2, Ki67, VEGFA, CD24 and CD44 expression decrease and Bax, caspase‐3 and ALDH1 expression increase after high‐dose CLO administration. MDA levels were substantially decreased in rat carcinomas in both CLO groups. The evaluation of histone modifications revealed increase in lysine trimethylations and acetylations (H4K20me3, H4K16ac) in carcinomas after CLO administration. TIMP3 promoter methylation levels of CpG3, CpG4, CpG5 islands were altered in treated cancer cells. An increase in total RASSF1A promoter methylation (three CpG sites) in CLO 1 group was found. In vitro studies showed antiproliferative and pro‐apoptotic effects of CLO extract in MCF‐7 cells (analyses of cytotoxicity, Brdu, cell cycle, annexin V/PI, caspase‐7, Bcl‐2 and mitochondrial membrane potential). This study showed a significant anticancer effect of clove buds in the mammary carcinoma model in vivo and in vitro.

Overexpression of TIMP3 Protects Against Cardiac Ischemia/Reperfusion Injury by Inhibiting Myocardial Apoptosis Through ROS/Mapks Pathway

“…Hence, inhibition of cardiomyocyte apoptosis can ameliorate myocardial dysfunction after I/R injury and limit myocardial remodeling development [32]. Although previous studies have revealed that TIMP3 plays an important role in regulating apoptotic activity in certain cancers [33, 34], prior to present study, it is unclear whether TIMP3 is involved in myocardial apoptosis undergoing I/R injury. The present data demonstrated that upregulation of TIMP3 inhibited myocardial apoptosis, as evidenced by reduced TUNEL-positive cells.…”

Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells

“…In our previous paper [12] we described anti-cancer activity of CBE extract in breast cancer model in vitro and in vivo. The results obtained pointed out to significant pro-apoptotic, antiproliferative and anti-angiogenic effects of CBE in mammary carcinoma model.…”

“…0.4% with no toxicity. Secondary metabolites identification in CBE extract was performed and characterised as was described in previous paper [12].…”

“…The study of Liu et al [11] showed the antiproliferative effect of clove extract against different types of cancer cells including breast, liver, ovarian or cervical. Recently, the anticancer effect of clove buds extract (CBE) was documented in a model of chemically-induced mammary carcinogenesis [12]. The above mentioned paper described a dose-dependent decrease in tumour frequency by 47.5% and 58.5% when compared to control.…”

Isobavachalcone sensitizes cells to E2‐induced paclitaxel resistance by down‐regulating CD44 expression in ER+ breast cancer cells

“…CD44 and CD24 are characteristic of the cancer stem cell phenotype, and these molecules are closely associated with poor prognosis and chemotherapy resistance in cancer. [24][25][26][27] Recently, natural substances from plants have been documented as effective intervention agents in the down-regulation of CD44/CD24 expression in experimental breast carcinoma. 28 However, whether IBC can directly regulate CD44/CD24 expression to decrease paclitaxel resistance in ER+ breast cancer cells remains unclear.…”