CisDDP treatment and development of the rat cerebellum

Scherini, Elda; Bernocchi, Graziella

doi:10.1016/0301-0082(94)90063-9

Cited by 23 publications

(27 citation statements)

References 71 publications

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“…Undifferentiated neuroepithelial cells are first recognized during embryogenesis in the primary germinal zone located in the neuroepithelial roof of the fourth ventricle [3]. These primitive cells migrate deep into the cerebellum and give rise to Purkinje cells [4]. A second germinal zone is located beneath the pia; the epithelial cells of this external granular layer give rise to granule cells of the cerebellum which then migrate into the internal granular cell layer [3,5,6].…”

Section: Molecular Imaging In Medulloblastomamentioning

confidence: 99%

Combining anatomic and molecularly targeted imaging in the diagnosis and surveillance of embryonal tumors of the nervous and endocrine systems in children

O’Dorisio

Khanna

Bushnell

2008

Cancer Metastasis Rev

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Combining anatomical and functional imaging can improve sensitivity and accuracy of tumor diagnosis and surveillance of pediatric malignancies. MRI is the state-of-the-art modality for demonstrating the anatomical location of brain tumors with contrast enhancement adding additional information regarding whether the tumor is neuronal or glial. Addition of SPECT imaging using a peptide that targets the somatostatin receptor (Octreoscan) can now differentiate medulloblastoma from a cerebellar pilocytic astrocytoma. Combined MRI and Octreoscan is now the most sensitive and accurate imaging modality for differentiating recurrent medulloblastoma from scar tissue. CT is the most common imaging modality for demonstrating the anatomical location of tumors in the chest and abdomen. Addition of SPECT imaging with either MIBG or Octreoscan has been shown to add important diagnostic information on the nature of tumors in chest and abdomen and is often more sensitive than CT for identification of metastatic lesions in bone or liver. Combined anatomical and functional imaging is particularly helpful in neuroblastoma and in neuroendocrine tumors such as gastrinoma and carcinoid. Functional imaging with MIBG and Octreoscan is predictive of response to molecularly targeted therapy with 131I-MIBG and 90Y-DOTA-tyr3-Octreotide. Dosimetry using combined anatomical and functional imaging is being developed for patient-specific dosing of targeted radiotherapy and as an extremely sensitive monitor of response to therapy. Both MIBG and Octreotide are now being adapted to PET imaging which will greatly improve the utility of PET in medulloblastoma as well as increase the sensitivity for detection of metastatic lesions in neuroblastoma and neuroendocrine tumors.

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Section: Molecular Imaging In Medulloblastomamentioning

confidence: 99%

Combining anatomic and molecularly targeted imaging in the diagnosis and surveillance of embryonal tumors of the nervous and endocrine systems in children

O’Dorisio

Khanna

Bushnell

2008

Cancer Metastasis Rev

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“…Further, although the lack of accumulation of both pNF-H or MAP2 in the Purkinje cell cytoplasm indicates that changes are not so massive as to lead to neuronal death (for a review see Scherini & Bernocchi 1994), degeneration signs deserve to be deeply investigated, while also studying potential recovery due to cell renewal.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the presence of pNF-H in the neuronal somata would be indicative of cellular damage such as in neurodegeneration events (Scherini & Bernocchi 1994).…”

Section: Cytoskeletal Proteins Heavy Phosphorylated Neurofilaments (Pmentioning

confidence: 99%

“…Within the MAP family, MAP2 is one of the most studied proteins; in the mammalian brain it is expressed mainly in dendrites and axons (Matus 1988;Riederer 1992;Ludin & Matus 1993;Riederer et al 1995), where it is involved in the formation of cross bindings between microtubules and neurofilaments and in microtubule assembly and stabilisation (Riederer 1990;Weisshaar & Matus 1993;Sánchez et al 2000). In rat cerebellar cortex, MAP2 labels cell somata, even though the strongest staining is evident in the Purkinje cell's main dendrite (Bernhardt & Matus 1984;Scherini & Bernocchi 1994); additionally, labelling is also identifiable in the ML (basket and stellate interneurons) as well as in the IGL (granule cells and Golgi cells) (Bernhardt & Matus 1984).…”

Section: Cytoskeletal Proteins Heavy Phosphorylated Neurofilaments (Pmentioning

confidence: 99%

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Changes in the cerebellar cytoarchitecture of hibernating hedgehogErinaceus europaeusL. (Mammalia): an immunocytochemical approach

Roda

Bottone

Insolia

et al. 2017

The European Zoological Journal

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Hibernation is an amazing animal strategy to survive when the environmental temperature is very low and food resources are scarce. Successful hibernation requires a variety of complex biological adaptations, in which the brain plays a central regulatory role. Currently, little information is available regarding the morphology and functional activity of specific neurons within the cerebellar cytoarchitecture of hibernating animals. In the present study, we investigated the immunohistochemical expression of essential proteins in the cerebellum of a mammalian hibernator (i.e. hedgehog Erinaceus europaeus L.), focusing on (i) Purkinje neurons, the sole output cells of the cerebellar cortex; (ii) selected neurotransmitters involved in hibernation processes; (iii) intracellular calcium homeostasis, considering that calcium is also an important regulator of neurotransmission mechanisms; and (iv) cytoskeletal proteins, involved in maintenance of neuronal shape and axon calibre. Specifically, we studied in situ immunocytochemical changes during the torpor state of hibernation (November-March) versus the activity phase (AprilSeptember). We employed different selected markers, i.e. glutamic acid decarboxylase (GAD67) and postsynaptic glutamate ionotropic receptor GluR2-3, different calcium-binding proteins (i.e. calbindin, parvalbumin and calretinin) and cytoskeletal components (i.e. pNF-H and MAP2). In summary, our data in hibernating animals demonstrated: (i) downregulation of GAD67, indicating loss/changes of synaptic contacts on Purkinje somata and dendrites; (ii) GluR2-3 upregulation in Purkinje neurons, with a drastic decrease of calbindin expression; and (iii) decrease of normal mechanisms regulating intracellular calcium homeostasis. We also found a decrease/modification in the distribution of cytoskeletal proteins, particularly evident for pNF-H. Changes in the functional activity of Purkinje cells were accompanied by some morphological dendrite alterations, signs of degeneration in cell somata and flattened basket pinceaux at the Purkinje axon hillock. These findings confirm that hibernation makes heterothermic animals a valuable model to study physiological adaptations to adverse conditions, and also for understanding cellular and molecular mechanisms aimed at preserving mammalian organs from full degeneration and death.

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“…In this work, the changes of autofluorescence properties during postnatal development in rat cerebellum were investigated under normal conditions and upon a mild treatment with the anti-tumoral drug cisplatin that is known to have neurotoxic side-effects [25][26][27], resulting in cerebellum histogenesis alterations [28][29][30]. The study was performed at defined time points of histogenesis, that is, at 11, 17, and 30 postnatal days (PD11, PD17, PD30), and attention was given to VI-VIII lobules of cerebellum vermis (neocerebellum), which undergo changes of differentiation phases when treatment is performed at the PD10 stage.…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence properties of rat cerebellum cortex during postnatal development

et al. 2006

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CisDDP treatment and development of the rat cerebellum

Cited by 23 publications

References 71 publications

Combining anatomic and molecularly targeted imaging in the diagnosis and surveillance of embryonal tumors of the nervous and endocrine systems in children

Combining anatomic and molecularly targeted imaging in the diagnosis and surveillance of embryonal tumors of the nervous and endocrine systems in children

Changes in the cerebellar cytoarchitecture of hibernating hedgehogErinaceus europaeusL. (Mammalia): an immunocytochemical approach

Autofluorescence properties of rat cerebellum cortex during postnatal development

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