Autofluorescence properties of rat cerebellum cortex during postnatal development

Croce, Anna Cleta; Pisu, Maria Bonaria; Roda, Elisa; Avella, Debora; Bernocchi, Graziella; Bottiroli, Giovanni

doi:10.1002/lsm.20336

Cited by 3 publications

(1 citation statement)

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“… 131-133 In this concern, the alteration of the postnatal development of the multilayered rat cerebellar cortex induced by cisplatin administration resulted in an increase in the lipofuscin emission signal and alterations in NAD(P)H and flavin contributions. 132 Actually, cerebellum during development is known to be particularly vulnerable to toxic compounds and radiation, and these findings in response to the cisplatin oxidative damage confirmed that AF-based studies are especially appropriate to investigate the oxidative effects of chemical and physical agent. 134 …”

Section: Lipofuscins and Lipofuscin-like Fluorophores In Aging Oxidamentioning

confidence: 90%

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

2014

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Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. This is a well-known phenomenon, and the strict relationship of many endogenous fluorophores with morphofunctional properties of the living systems, influencing their AF emission features, offers an extremely powerful resource for directly monitoring the biological substrate condition. Starting from the last century, the technological progresses in microscopy and spectrofluorometry were convoying attention of the scientific community to this phenomenon. In the future, the interest in the autofluorescence will certainly continue. Current instrumentation and analytical procedures will likely be overcome by the unceasing progress in new devices for AF detection and data interpretation, while a progress is expected in the search and characterization of endogenous fluorophores and their roles as intrinsic biomarkers.

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Section: Lipofuscins and Lipofuscin-like Fluorophores In Aging Oxidamentioning

confidence: 90%

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

2014

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Optical spectroscopy for in vivo medical diagnosis—a review of the state of the art and future perspectives

2020

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When light is incident to a biological tissue surface, combinations of optical processes occur, such as reflection, absorption, elastic and non-elastic scattering, and fluorescence. Analysis of these light interactions with the tissue provides insight into the metabolic and pathological state of the tissue. Furthermore, in vivo diagnosis of diseases using optical spectroscopy enables in situ rapid clinical decisions without invasive biopsies. For in vivo scenarios, incident light can be delivered in a highly localized manner to tissue via optical fibers, which are placed within the working channels of minimally invasive clinical tools, such as endoscopes. There has been extensive development in the accuracy and specificity of these optical spectroscopy techniques since the earliest in vivo examples were published in the academic literature in the early ‘90s, and there are now commercially available systems that have undergone medical and clinical trials. In this review, several types of optical spectroscopy techniques (elastic optical scattering spectroscopy, fluorescence spectroscopy, Raman spectroscopy, and multimodal spectroscopy) for the diagnosis and monitoring of diseases states of tissue in an in vivo setting are introduced and explored. Examples of the latest and most impactful works for each technique are then critically reviewed. Finally, current challenges and unmet clinical needs are discussed, followed by future opportunities, such as point-based spectroscopies for robot-guided surgical interventions.

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Lipofuscin, lipofuscin-like pigments and autofluorescence

Guardo

2015

Eur J Histochem

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Autofluorescence properties of rat cerebellum cortex during postnatal development

Abstract: The results confirm the relationship of the autofluorescence emission properties with histological and biochemical features of biological tissue.

Cited by 3 publications

References 50 publications

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

Optical spectroscopy for in vivo medical diagnosis—a review of the state of the art and future perspectives

Lipofuscin, lipofuscin-like pigments and autofluorescence

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