Cirrose experimental induzida pela inalação de tetracloreto de carbono: adaptação da técnica e avaliação da peroxidação lipídica

Rv, Cremonese; Aa, Pereira-Filho; Magalhães, Renato M.; Aa, de Mattos; Ca, Marroni; Cg, Zettler; Np, Marroni

doi:10.1590/s0004-28032001000100008

Cited by 11 publications

(8 citation statements)

References 23 publications

(40 reference statements)

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“…In the fourth session, the length of gas administration was increased to 1 minute, followed by another minute in the waiting mode. Subsequently, the length of gas administration and the waiting period in the chamber were increased by 30 s every three sessions, up to a peak of 5 min at 16 weeks, according to the method adapted from Cremonese et al [14]. …”

Section: Methodsmentioning

confidence: 99%

Effect of Antioxidant Treatment on Fibrogenesis in Rats with Carbon Tetrachloride-Induced Cirrhosis

Bona

Filippin

Naso

et al. 2012

ISRN Gastroenterology

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Aim. This study aimed to assess the antioxidant activity of quercetin (Q) in an experimental model of cirrhosis induced by CCl4 inhalation. Materials and Methods. We used 25 male Wistar rats (250 g) that were divided into 3 groups: control (CO), CCl4, and CCl4 + Q. The rats were subjected to CCl4 inhalation (2x/week) for 16 weeks, and they received phenobarbital in their drinking water at a dose of 0.3 g/dL as a P450 enzyme inducer. Q (50 mg/Kg) was initiated intraperitoneally at 10 weeks of inhalation and lasted until the end of the experiment. Statistical analysis was by ANOVA Student Newman-Keuls (mean ± SEM), and differences were considered statistically significant when P < 0.05. Results. After treatment with quercetin, we observed an improvement in liver complications, decreased fibrosis, as analyzed by picrosirius for the quantification of collagen, and decreased levels of matrix metalloproteinase 2 (MMP-2) compared with the CCl4 group. It also reduced oxidative stress, as confirmed by the decrease of substances reacting to thiobarbituric acid (TBARS), the increased activity of antioxidant enzymes, and the reduced glutathione ratio and glutathione disulfide (GSH/GSSG). Conclusion. We suggest that the use of quercetin might be promising as an antioxidant therapy in liver fibrosis.

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Section: Methodsmentioning

confidence: 99%

Effect of Antioxidant Treatment on Fibrogenesis in Rats with Carbon Tetrachloride-Induced Cirrhosis

Bona

Filippin

Naso

et al. 2012

ISRN Gastroenterology

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“…at a dose of 10 mg/kg/day (42) . It was initiated at week 10th, when histological analyses and liver function tests indicated that the animals were already cirrhotic, and was carried out until the date of sacrifice (6) .…”

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confidence: 99%

“…The model used in this work was a modified version of the one proposed by JIMÉNEZ and CLÁRIA (5) , the main difference being the gradual increase in the gassed time -by 30 seconds every three sessions -thus producing progressive disarray in the hepatic parenchyma without leading to the pronounced mortality as initially observed, when 1-minute increases in the inhalation time were employed (6) . Lipoperoxidation is a marker for the damage to cell membranes and, in the TBA-RS method, it is measured through the formation of an intermediate compound of lipoperoxidation, malondialdehyde.…”

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confidence: 99%

Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats

Pereira-Filho

Ferreira

Schwengber

et al. 2008

Arq. Gastroenterol.

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-Background -Hepatic cirrhosis is the final stage of liver dysfunction, characterized by diffuse fibrosis which is the main response to the liver injury. The inhalatory carbon tetrachloride is an effective experimental model that triggers cirrhosis and allows to obtain histological and physiological modifications similar to the one seen in humans. Aim -To investigate the effects of N-acetylcysteine (NAC) on the fibrosis and oxidative stress in the liver of cirrhotic rats, analyzing liver function tests, lipoperoxidation, activity of glutathione peroxidase enzyme, collagen quantification, histopathology, as well as the nitric oxide role. Methods -The animals were randomly in three experimentals groups: control (CO); cirrhotic (CCl4) and CCl4 + NAC. Evaluate the lipid peroxidation, the glutathione peroxidase enzyme, the collagen and the expression of inducible nitric oxide synthase (iNOS). Results -The cirrhotic group treated with N-acetylcysteine showed trough the histological analysis and collagen quantification lower degrees of fibrosis. This group has also shown less damage to the cellular membranes, less decrease on the glutathione peroxidase levels and less expression of inducible nitric oxide synthase when matched with the cirrhotic group without treatment. Conclusion -N-acetylcysteine seams to offer protection against hepatic fibrosis and oxidative stress in cirrhotic rat livers.

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“…Entretanto, desvantagens, como a presença de septos fibrosos, pouca proliferação ductobiliar e rápida regressão das lesões dificultam o estudo de tratamentos anticirróticos em longo prazo (Cremonese et al, 2001;Li et al, 2002;Muriel e Escobar, 2003), devido à baixa reprodutibilidade e homogeneidade do quadro cirrótico (Laleman et al, 2006) e alto índice de mortalidade encontrados durante a indução (Jeong et al, 2001;Li et al, 2002;Laleman et al, 2006;Lee et al, 2008). Yongping et al (2009) obtiveram uma porcentagem de 20% de ratos que não apresentaram cirrose hepática, esse dado, adicionado a outros fatos supracitados, demonstra que o CCl 4 não é totalmente eficaz na indução da cirrose.…”

Section: Análise Comparativa Dos Modelos Cirróticos: Vantagens E Desvunclassified

<b>Modelos experimentais para indução de cirrose hepática em animais: Revisão de literatura</b><br>doi: 10.5007/2175-7925.2010v23n2p183

et al. 2011

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ResumoO fígado desempenha papel homeostático fundamental no equilíbrio de numerosos processos biológicos. Cirrose hepática é uma síndrome, na qual convergem algumas doenças hepáticas crônicas, ocorrendo lesão hepatocelular seguida de deposição exacerbada de tecido fibroso ocasionando desorganização da arquitetura tecidual. O fígado está sujeito à lesão potencial por uma grande quantidade de agentes farmacológicos, tóxicos e/ou microbiológicos. Para o estudo de possíveis tratamentos para a cirrose, é necessário o estabelecimento de modelos animais de indução de cirrose, principalmente em roedores de laboratório que mimetizem o processo cirrótico encontrado em animais e homem, que tenham alta reprodutibilidade, homogeneidade e baixa mortalidade. Sendo assim, a indução de cirrose hepática torna-se primordial para investigar doenças hepáticas crônicas, como também para testar possíveis tratamentos terapêuticos para posterior utilização na clínica veterinária e humana. Tetracloreto de Carbono-CCl 4 , Tiocetamida -TAA e Dimetilnitrosamina -DMN têm sido as drogas de eleição para a indução da cirrose experimental em ratos, e são os modelos analisados neste trabalho. O modelo cirrótico com a TAA se mostrou ser o mais promissor pelos seguintes motivos: produz padrão histológico mais próximo ao da cirrose humana, com menor mortalidade, maior reprodutibilidade e segurança, apesar do período de indução ser maior (14 semanas).Unitermos: cirrose hepática, indução, modelos experimentais, rato, tioacetamida AbstractExperimental models for induction of liver cirrhosis in animals: a review. The liver plays a key role in the homeostatic balance of many biological processes. Cirrhosis is a syndrome in which chronic liver diseases converge, leading to hepatocellular injury, the exacerbated deposition of fibrous tissue, and eventually the disruption of the tissue architecture. The liver is subject to potential injury by a large quantity of pharmacological Biotemas, 23 (2): 183-190, junho de 2010

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Cirrose experimental induzida pela inalação de tetracloreto de carbono: adaptação da técnica e avaliação da peroxidação lipídica

Cited by 11 publications

References 23 publications

Effect of Antioxidant Treatment on Fibrogenesis in Rats with Carbon Tetrachloride-Induced Cirrhosis

Effect of Antioxidant Treatment on Fibrogenesis in Rats with Carbon Tetrachloride-Induced Cirrhosis

Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats

<b>Modelos experimentais para indução de cirrose hepática em animais: Revisão de literatura</b><br>doi: 10.5007/2175-7925.2010v23n2p183

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