Circulating Trophoblast Cells in Maternal Peripheral Blood

Wijk, Inge J. van; Tjoa, May Lee; Griffioen, S.; Mulders, Monique A.M.; Vugt, J.M.G. van; Oudejans, Cees B.M.

doi:10.1159/000062528

Cited by 3 publications

(2 citation statements)

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“…The understanding that fetal RNA in maternal plasma is predominantly placental in origin with easy and reliable detection of human placental lactogen (hPL) 3 mRNA in all pregnancy stages is in accordance with the fact that the major cell type circulating in the maternal blood is of trophoblastic, in particular extravillus, origin (5 ). The apparently physiologic manner by which components (i.e., nuclei and mRNA) (1,6,7 ) of these cells appear in the maternal plasma is unknown, although programmed cell death (apoptosis) seems to be involved (6,7 ).…”

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confidence: 84%

Detection of Chromosome 21-encoded mRNA of Placental Origin in Maternal Plasma

Oudejans¹,

Visser³

et al. 2003

Clinical Chemistry

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Background: mRNA of placental origin (i.e., human placental lactogen and ␤-human chorionic gonadotropin) has been demonstrated to be easily detectable in maternal plasma. We tested whether detection of chromosome 21-encoded mRNA of placental origin is possible in maternal plasma obtained during the first trimester. Methods: Plasma samples were obtained from pregnant women between weeks 9 -13 of pregnancy. RNA was isolated from 800 or 1600 L of plasma by silica-based affinity isolation and, after on-column DNase treatment, was subjected to two-step, one-tube reverse transcription-PCR with gene specific primers. Results: Three chromosome 21-encoded genes located within the Down syndrome critical region with overexpression in trisomy 21 placentas were screened for expression in early placental tissue to select their potential use for RNA based plasma screening. One of the chromosome 21-encoded genes (LOC90625) showed strong expression in first trimester placenta similar to CSH1 (human placental lactogen) and was selected for plasma analysis. The RNA isolation assay was validated with CSH1 mRNA, which could be detected in the plasma of all women tested in weeks 9 -13 of pregnancy. RNA from the chromosome 21-encoded, placentally expressed gene, LOC90625, was present in maternal first-trimester plasma and could be detected in 60% of maternal plasma samples when 800 L of plasma was used and in 100% of samples when 1600 L of plasma was used. Conclusion: The detection of chromosome 21-encoded mRNA of placental origin in maternal plasma during the first trimester may allow development of plasma-RNA-based strategies for prenatal prediction of Down

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confidence: 84%

Detection of Chromosome 21-encoded mRNA of Placental Origin in Maternal Plasma

Oudejans¹,

Visser³

et al. 2003

Clinical Chemistry

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show abstract

“…Διάφορα μονοκλωνικά αντισώματα έχουν χρησιμοποιηθεί για την απομόνωσή τους, όπως αντισώματα έναντι αντιγόνων του ανθρώπινου λευκοκυτταρικού αντιγόνου HLA-G (HLA-MEM-G/9, G233 και 4H48), αντισώματα έναντι του πλακουντικού παράγοντα αύξησης (PlGF), του μεταγραφικού παράγοντα neuroD2 και αντισώματα έναντι δεικτών επιφανείας, όπως τα MΑb-340, GZ112 και GZ158, όμως από τα μέχρι σήμερα στοιχεία της βιβλιογραφίας δεν προκύπτει ότι κάποιο από αυτά είναι πολύ ειδικό ώστε να χρησιμοποιηθεί για την απομόνωση των κυττάρων αυτών από το περιφερικό αίμα της εγκύου (33,34,35,36,37).…”

Section: τροφοβλαστικά κύτταραunclassified