Circulating P-Selectin Glycoprotein Ligand 1 and P-Selectin Levels in Obstructive Sleep Apnea Patients

Abstract

Purpose Obstructive sleep apnea (OSA) is characterized by chronic intermittent hypoxia which induces inflammation in blood vessels leading to the development of cardiovascular comorbidities. Several studies implicated the role of P-selectin in vascular inflammation of OSA. P-selectin glycoprotein ligand 1 (PSGL-1) is the main activator for P-selectin and is involved in immune cell trafficking. However, PSGL-1 has not been analyzed in OSA. The aim of the study was to investigate plasma PSGL-1 and P-selectin levels to have a deeper understanding on their interaction in obstructive sleep apnea. Methods Fifty-one untreated patients with OSA and 42 non-OSA controls were recruited. Plasma PSGL-1 levels were determined in evening and morning samples, P-selectin levels were analyzed in morning samples using commercially available ELISA kits. Polysomnography was performed in all participants. OSA was defined by an apnea-hypopnea index ≥ 5/h. Results PSGL-1 levels did not differ between controls and OSA patients either in the evening or in the morning. Although, there was no difference between controls (16.9/6.8-40.8 ng/ml) and patients with OSA (19.6/8.4-56.8, p = 0.24), patients with severe OSA had increased plasma P-selectin levels (25.6/8.4-56.8 ng/ml) compared to mild OSA patients (14.1/8.5-35.3 ng/ ml, p = 0.006) and controls (p = 0.03). Conclusions P-selectin expression relates to disease severity suggesting a pathophysiological role in endothelial cell activation. PSGL-1 levels are unaltered in OSA, suggesting an alternative activation pathway for P-selectin in OSA.

The role of hyaluronic acid and hyaluronidase-1 in obstructive sleep apnoea

“…The degradation of HMW-HA under inflammatory conditions reduces extracellular HA viscosity and increase tissue permeability 34 resulting extravasation of macrophages to the arterial wall. The damaged endothelium facilitates the adhesion of leukocytes and platelets via adhesion molecules, and the levels were found to be elevated in OSA 2,35 . Dogné et al demonstrated that the lack of HYAL-1 is associated with lower levels of P-selectin suggesting that HYAL-1 deficiency may be protective in endothelial dysfunction 33 .…”

“…Obstructive sleep apnoea (OSA) is the most common sleep-related breathing disorder which is characterised by the repetitive collapse of the upper airways during sleep resulting in chronic intermittent hypoxaemia (CIH) and frequent arousals with sleep fragmentation. These factors lead to enhanced oxidative stress and consequential systemic inflammation which are associated with altered concentrations of circulating pro-inflammatory 1 , 2 and anti-inflammatory 3 , 4 biomarkers. Understanding the role of inflammation in OSA is of importance as it could be a link to the development of cardiometabolic comorbidities.…”

Obstruktív alvási apnoéban szenvedő betegek jellemzői hazai beteganyagon

“…Korábbi kutatásokhoz ha-sonlóan a CRP-szintek vizsgálatunkban is szignifikánsan magasabbnak bizonyultak az OSA-ban szenvedő résztvevők körében a kontrollcsoporthoz képest [26]. A CRP mellett számos proinflammatorikus citokin és biomarker (például adhéziós molekulák, komplementek) magasabb szintjét igazolták OSA-ban [27,28].…”

LUNG Year in Review: 2020

“…In 2020, we published two articles on pediatric obstructive sleep apnea syndrome (OSAS) [ 5 , 27 ], including a State-of-the-Art review on the evaluation and management of children with OSAS [ 5 ]. Studies in adult OSAS included therapy [ 79 ], adherence [ 76 ], risk of occupational injury [ 78 ], and possible associated biomarkers [ 75 , 77 , 80 ].…”