Circulating miRNAs—A potential tool to identify severe asthma risk?

Kyyaly, Mohammed Aref; Sánchez-Elsner, Tilman; He, Peijun; Sones, C.L.; Arshad, Syed Hasan; Kurukulaaratchy, Ramesh

doi:10.1002/clt2.12040

Cited by 7 publications

(6 citation statements)

References 8 publications

(8 reference statements)

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“…Several studies have attempted to use miRNAs for asthma severity determination in biofluids. MiR-28-3p, miR-16-2-3p, and miR-210-3p have recently been associated with severe asthma and were suggested as biomarkers, although the study was only preliminary (33). Other reports have elucidated profiles of miRNAs in sputum related to neutrophilic severe asthma, of which miR-223-3p was found to be a key node (17).…”

Section: Discussionmentioning

confidence: 92%

miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma

et al. 2022

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MicroRNAs are non-coding molecules that act both as regulators of the epigenetic landscape and as biomarkers for diseases, including asthma. In the era of personalized medicine, there is a need for novel disease-associated biomarkers that can help in classifying diseases into phenotypes for treatment selection. Currently, severe eosinophilic asthma is one of the most widely studied phenotypes in clinical practice, as many patients require higher and higher doses of corticosteroids, which in some cases fail to achieve the desired outcome. Such patients may only benefit from alternative drugs such as biologics, for which novel biomarkers are necessary. The objective of the study was to study the expression of miR-144-3p in order to discover its possible use as a diagnostic biomarker for severe asthma. For this purpose, miR-144-3p was evaluated in airway biopsies and serum from asthmatics and healthy individuals. mRNA was studied in asthmatic biopsies and smooth muscle cells transfected with miR-144-3p mimic. An in silico regulation of miR-144-3p was performed using miRSystem, miRDB, STRING, and ShinyGO for pathway analysis. From our experimental procedures, we found that miR-144-3p is a biomarker associated with asthma severity and corticosteroid treatment. MiR-144-3p is increased in asthmatic lungs, and its presence correlates directly with blood eosinophilia and with the expression of genes involved in asthma pathophysiology in the airways. When studied in serum, this miRNA was increased in severe asthmatics and associated with higher doses of corticosteroids, thereby making it a potential biomarker for severe asthma previously treated with higher doses of corticosteroids. Thus, we can conclude that miR-144-3p is associated with severe diseases in both the airways and serum of asthmatics, and this association is related to corticosteroid treatment.

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Section: Discussionmentioning

confidence: 92%

miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma

et al. 2022

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“…His study included only four adult BA patients in each group, making our data statistically more credible. 9 Two members of the miR-146 family, miR-146a and miR-146b are expressed as negative feedback in response to proinflammatory stimuli to prevent excessive inflammation. 31 In this study, the expression of miR146a-5p was significantly upregulated in the mild to moderate group compared to the average expression by 5.8-fold (P = 0.001).…”

Section: Discussionmentioning

confidence: 99%

“…7 Observed modifications of miRs reported in BA in easily accessible body fluids (serum and plasma) have suggested miRs as biomarkers for disease diagnosis 8 and identifying patients at risk for severe asthma. 9 According to reports, miR-210-3p inhibits the protein expression of Foxp3 and modulates the immunosuppressive properties of Tregs which induces immune dysfunction via T regs in Psoriasis vulgaris patients, 10,11 but this link has not been investigated in childhood BA.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-146a-5p and microRNA‐210‐3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population

Eldosoky

Hammad

Rushdi

et al. 2023

JAA

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Background: Severe bronchial asthma (BA) affects 5-10% of children, which imposes socioeconomic burden. Therefore, it is crucial to identify biomarkers for risk stratification in children with BA. T regulatory cells (Tregs) play a balancing role in allergic response regulation. We aimed to investigate the relationship between Treg, miR-210-3p, and miR-146a-5p in relation to asthma phenotypes in search of novel biomarkers of disease severity. Methods: This study included 50 children with BA classified into Group 1 (n = 25) children with mild to moderate asthma and Group 2 (n = 25) children with severe asthma. In addition to 26 control subjects. Flow cytometry was used to detect Tregs. Plasma miR-210-3p and miR-146a levels were determined using quantitative real-time PCR. Patients' FEV1 (Forced Expiratory Volume in the first second) was measured. Results: miR-210-3p level correlated negatively with Treg frequency (r = −0.828, P < 0.001) and FEV1 (r = −0.621, P < 0.001). The level of miR-146a-5p positively correlated positively with Treg% (r = 0.303, P = 0.032). ROC curve analysis revealed that miR-210-3p was the most sensitive biomarker of severity, with the area under curve (AUC) = 0.923, 96% sensitivity, and 60% specificity. According to multivariate analysis, miR-210-3p is an independent risk factor for BA severity [OR =3.119, P = 0.030], while miR-146a-5p is a protective factor [OR =0.811, P = 0.049]. Conclusion:Treg frequency is linked to FEV1, miR-146a-5p and miR-210-3p in childhood BA. Upregulation of miR-210-3p is a sensitive biomarker and an independent risk factor for BA severity in Egyptian children.

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“…Недавние исследования показали, что miR-28-3p, -16-2-3p, -210-3p, -185, -125b, -338-3p и -125b могут быть маркерами тяжелой БА [18,19]. J.M.…”

Section: микрорнк в контексте патогенеза бронхиальной астмыunclassified

The role of microRNA in the pathogenesis of bronchoobstructive diseases

et al. 2023

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The review analyzes the role of microRNAs in the pathogenesis of bronchopulmonary diseases. The universality of the mechanisms underlying epigenetics causes a continuously growing interest in research in this field in various fields of medicine. Research in the field of epigenetics not only allows us to expand knowledge in the field of etiology and pathogenesis, but also helps to explain the heterogeneity of the disease. Currently, biomarkers used in determining the phenotype of bronchial asthma or COPD are not able to display the variety of pathological processes involved in the pathogenesis of the disease at the molecular level. It is noteworthy that microRNAs retain their stability in various body environments, are resistant to high temperatures, pH fluctuations, and freeze-thaw cycles, which greatly simplifies the process of detecting these molecules in biological fluids. The amount of detected microRNA is highly specific for a particular pathological process occurring intracellularly. Currently, biomarkers used in determining the phenotype of bronchial asthma or chronic obstructive pulmonary disease are not able to reflect the variety of pathological processes involved in the pathogenesis of the disease at the molecular level. For both diseases, the key links are known to be inflammation, airway remodeling, and an abnormal response of epithelial cells to external stimuli. Thus, there is a great potential for using microRNAs in clinical practice: as noninvasive biomarkers reflecting key points of pathogenesis, as a prognostic biomarker predicting response to therapy, and possibly in the future as new therapeutic targets.

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Circulating miRNAs—A potential tool to identify severe asthma risk?

Cited by 7 publications

References 8 publications

miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma

miR-144-3p Is a Biomarker Related to Severe Corticosteroid-Dependent Asthma

MicroRNA-146a-5p and microRNA‐210‐3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population

The role of microRNA in the pathogenesis of bronchoobstructive diseases

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