Circulating miR-130a-3p is elevated in patients with cerebral atherosclerosis and predicts 2-year risk of cerebrovascular events

Abstract

Background Cerebral atherosclerosis (AS) leads to high risk of cerebrovascular events. This study aims to evaluate the diagnostic performance of serum microRNA-130a-3p (miR-130a-3p) in cerebral AS patients, and construct a logistic risk model for 2-year cerebrovascular events on the basis of the prognostic potential of miR-130a-3p. Methods Serum samples were collected from 74 cerebral AS patients and 62 control individuals, and miR-130a-3p expression was investigated using reverse transcription quantitative PCR. Risk factors related with cerebral AS were assessed using a logistic regression analysis, and the receiver operating characteristic analysis was performed to evaluate the diagnostic value of miR-130a-3p. The relationship between miR-130a-3p and cerebrovascular events was analyzed using a Kaplan–Meier method, and a logistic risk model was constructed for 2-year cerebrovascular events. Results Cerebral AS patients had elevated serum miR-130a-3p compared with controls (P < 0.001). Serum miR-130a-3p had diagnostic value (AUC = 0.899), and could significantly improve the diagnostic accuracy of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in cerebral AS patients (AUC = 0.992). High serum miR-130a-3p was independently related with high probability of cerebrovascular events (HR = 1.993, 95% CI = 1.205–2.897, P = 0.006), and a logistic risk model was constructed based on serum miR-130a-3p, hs-CRP, TC and LDL-C. Conclusion All the findings indicated that high serum miR-130a-3p had diagnostic potential to screen cerebral AS, and predicted the probability of cerebrovascular events after AS. The logistic risk model based on miR-130a-3p may provide an efficient method to predict 2-year cerebrovascular events in AS patients.

The expression levels of circulating miR‐140‐3p, miR‐130a‐3p, and miR‐320b as diagnostic biomarkers in acute ischemic stroke

“…It has also been proposed as a plasmatic biomarker for several diseases, 16,17 including mild cognitive impairment 18 . MiR‐130a‐3p (accession: MIMAT0000425) was elevated in serum from patients with cerebral atherosclerosis 19 and tended to show higher plasma levels in atrial fibrillation patients with high CHA2DS2‐VAS scores (a tool for stratifying AF patient with risk of stroke 20 ) and lower plasma levels in patients who underwent catheter ablation 21 . MiR‐320b (accession: MIMAT0005792) is a member of the miR‐320 family and its lower levels of plasma miR‐320b were associated with increased occurrence of acute myocardial infarction in Chinese populations 22 .…”

Extracellular vesicles in atherosclerosis and vascular calcification: the versatile non-coding RNAs from endothelial cells and vascular smooth muscle cells

“…Downregulation of miR-130a-3p expression leads to endothelial barrier dysfunction in the development of AS ( 161 ). Paradoxically, serum miR-130a-3p was elevated in cerebral AS patients and provided a possible method to predict cerebrovascular events ( 162 ). The different expressions of miR-130a-3p in AS period indicated the potential ability in AS diagnosis.…”