Circulating microRNAs in patients with aneurysmal dilatation of coronary arteries

Abstract

To understand the mechanism underlying coronary artery abnormal dilatation (CAAD), the present study identified and compared the expression of circulating microRNAs (miRNAs) in three groups of patients. Group 1 included 20 patients with CAAD, Group 2 included 20 patients with angiographically confirmed coronary artery disease (CAD), and Group 3 included 20 patients with normal coronary arteries (control). miRNAs were isolated from plasma samples and were profiled using PCR arrays and miRCURY LNA Serum/Plasma Focus PCR Panels. The present study demonstrated that the plasma miRNA levels were significantly different in Group 1 compared with in Group 2 and Group 3 (fold change >2 and P<0.05). The comparison of Group 1 with Group 3 identified 21 significantly upregulated and two downregulated miRNAs in patients with CAAD compared with in the control group. Moreover, six upregulated and two downregulated miRNAs were identified in patients with CAD compared with in the controls. The third comparison revealed four upregulated and three downregulated miRNAs in Group 1, when compared with patients with CAD. In conclusion, the present study identified a specific signature of plasma miRNAs, which were upregulated and downregulated in patients with CAAD compared with in patients with CAD and control individuals.

Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

“…miRNAs play a role in the mechanism of atherosclerosis, and atherosclerotic coronary artery disease is characterized by specific signatures of miRNA in tissue and plasma assays [ 23 , 24 , 25 , 26 ]. However, scarce data concerning miRNAs involved in CAAD pathology prompted us to study five circulating miRNAs: MiR-125b-5p, miR-210-3p, miR-328-3p, miR-425-3p, and miR-483-5p, which we preselected and described in our former study [ 14 ] as potentially associated with CAAD.…”

“…We applied the RT-qPCR method using a PCR array system. The custom plates for the RT-qPCR array were designed as in our former study [ 14 ].…”

“…Our previous study precisely described obtaining and preparing blood samples [ 14 ]. We treated the new collection of blood samples according to the same procedure described here.…”

“…Based on our previous study [ 14 ], we selected the potential miRNA markers to validate them in a larger independent patient cohort. The present analysis aims to confirm the differences in the expression of five preselected miRNA markers (miR-125b-5p, miR-210-3p, miR-328-3p, miR-425-3p, and miR-483-5p), and three reference miRNAs (miR-16-5p, miR-30d-5p, miR-320d) in larger study groups and evaluate their usefulness as potential markers of CAAD occurrence.…”