Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

Abstract

MicroRNAs (miRNAs) are currently investigated as crucial regulatory factors which may serve as a potential therapeutic target. Reports on the role of miRNA in patients with coronary artery aneurysmal disease (CAAD) are limited. The present analysis aims to confirm the differences in the expression of previously preselected miRNAs in larger study groups and evaluate their usefulness as potential markers of CAAD. The study cohort included 35 consecutive patients with CAAD (Group 1), and two groups of 35 patients matched Group 1 regarding sex and age from the overall cohort of 250 patients (Group 2 and Group 3). Group 2 included patients with angiographically documented coronary artery disease (CAD), while Group 3 enrolled patients with normal coronary arteries (NCA) assessed during coronary angiography. We applied the RT-qPCR method using the custom plates for the RT-qPCR array. We confirmed that the level of five preselected circulating miRNAs was different in patients with CAAD compared to Group 2 and Group 3. We found that miR-451a and miR-328 significantly improved the CAAD prediction. In conclusion, miR-451a is a significant marker of CAAD compared to patients with CAD. In turn, miR-328-3p is a significant marker of CAAD compared to patients with NCA.

Differential Expression of Circulating Damage-Associated Molecular Patterns in Patients with Coronary Artery Ectasia

“…Additionally, increased miR328a-3p levels positively correlated with increased risk of heart failure or mortality within 6 months [27]. Increased levels of miR-328a-3p have also been reported in patients with coronary artery aneurysmal disease compared to healthy controls [29].…”

Lifestyle-Driven Variations in Nutrimiromic MicroRNA Expression Patterns across and beyond Genders

“…These outlined mechanisms could similarly account for the noted elevation in miRNA-328 levels; however, the effects of the observed increase in miRNA-328 expression remain unclear given its multifaceted role in various tumour types. While it acts as a tumour suppressor in various cancers by inhibiting tumour progression, miRNA-328 paradoxically acts as an onco-miRNA in ovarian cancer, highlighting the complexity of its function in oncogenesis and the need for further research to decipher its diverse effects [ 94 , 95 , 96 , 97 , 98 ].…”

Coronary Artery Ectasia Database — Poland (CARED-POL). The rationale and design of the multicenter nationwide registry

“…Although some pathophysiological and clinical risk factors for CAAE development have been identified, detailed pathomechanisms have not yet been known [8][9][10]. Moreover, so far, the data on Polish patients are limited to case reports, case series, and small groups from major academic centers [8][9][10][11][12][13]. CAAEs are not analyzed in the main nationwide registers of invasive procedures either [14].…”