Circulating microRNAs in Cardiovascular Diseases

Orlicka-Płocka, Marta; Gurda, Dorota; Fedoruk-Wyszomirska, Agnieszka; Smolarek, Iwona; Wyszko, Eliza

doi:10.18388/abp.2016_1334

Cited by 8 publications

(7 citation statements)

References 47 publications

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“…This regulation is also present in the self-renewal of stem cells and the differentiation of a variety of cell lineages (7). It is confirmed that the generation of miRNAs can interfere with the process of angiogenesis and the function of vascular endothelial cells (8).…”

Section: Introductionmentioning

confidence: 78%

miR‑214 mediates vascular inflammation and apoptosis via PTEN expression

Wang

Liu

et al. 2018

Mol Med Report

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The present study aimed to investigate the role of miR‑214 on inflammation and apoptosis in the vascular system and to examine its potential mechanisms. Anti‑miR‑214 mimics were used to downregulate miR‑214 expression in HUVECs. Cell viability and the apoptosis rate were measured using MTT assay and flow cytometry. Tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6 and IL‑18 levels were measured using ELISA kits. Following this, caspase‑3/9, Bax, phosphatase and tensin homolog (PTEN), nuclear factor (NF)‑κB and phosphorylated‑(p)‑protein kinase B (Akt) protein expression were analyzed using western blotting. The results demonstrated that anti‑miR‑214 mimics inhibited cell proliferation, increased apoptosis and inflammatory factors (TNF‑α, IL‑1β, IL‑6 and IL‑18 levels), inhibited cell proliferation, and induced Bax protein expression in TNF‑α‑induced vascular endothelial cells through induction of PTEN and NF‑κB protein expression and inhibition of Akt protein expression. The PTEN inhibitor inhibited the function of anti‑miR‑214 on apoptosis and inflammation in TNF‑α‑induced inflammation vascular endothelial cells through the PTEN/Akt signaling pathway. These results suggest that miR‑214 mediates vascular inflammation and apoptosis via PTEN expression.

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Section: Introductionmentioning

confidence: 78%

miR‑214 mediates vascular inflammation and apoptosis via PTEN expression

Wang

Liu

et al. 2018

Mol Med Report

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“…Specifically, the Human Cardiovascular Disease miRNA array (Qiagen, Germany) was examined in 20 individuals from the HERITAGE Family Study. This array includes 84 miRNAs that are differentially regulated during CVD progression and have been associated with CVD in multiple studies involving a variety of CVD models and a variety of miRNA profiling methodologies 20–28 . We hypothesized that regular exercise would significantly alter the circulating miRNAs related to CVD.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Regular Exercise on Circulating Cardiovascular-related MicroRNAs

Barber

Zellars

Barringhaus

et al. 2019

Sci Rep

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The purpose of the present study was to examine the effects of regular exercise on the abundance of targeted circulating microRNAs (miRNAs). The present analysis examined 20 previously sedentary adults from the HERITAGE Family Study who completed 20 weeks of endurance exercise training. The expression of 53 miRNAs related to cardiovascular disease were measured in serum collected at baseline and post-training by performing RT-qPCR on the Human Cardiovascular Disease miRNA array (Qiagen, Germany). The effect of regular exercise on circulating miRNAs was assessed using paired t-tests of baseline and post-training expression levels. A false discovery rate threshold of 5% was used to determine significance. Regular exercise resulted in significantly decreased mean serum expression of nine miRNAs (miR-486-5p, let-7b-5p, miR-29c-3p, let-7e-5p, miR-93-5p, miR-7-5p, miR-25-3p, miR-92a-3p, and miR-29b-3p; fold change range: 0.64–83, p = 0.0002–0.01) and increased mean expression of five miRNAs (miR-142-3p, miR-221-3p, miR-126-3p, miR-146a-5p, and miR-27b-3p; fold change range: 1.41–3.60, p = 0.001–0.006). Enrichment analysis found that these 14 miRNAs target genes related to over 345 different biological pathways. These results provide further evidence of the effects of regular exercise on the circulating miRNA profile.

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“…Over the past few years, researchers have been focusing on the role of microRNAs in cellular, molecular, and various disorders and diseases (17,18).…”

Section: Discussionmentioning

confidence: 99%

Relationship between microRNA-206 plasma levels with the severity of coronary artery conflicts in patients with coronary artery disease

Zehtabian¹,

Alibakhshi²,

Seyedena³

et al. 2019

BLL

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OBJECTIVES: The aim of this study was to evaluate the possible association of miR-206 serum as an indicator of diagnosis in patients with coronary artery disease. METHODS: In this study, 100 patients with coronary artery disease who had angiography and vascular transplantation were selected and evaluated. Extraction of microRNAs from peripheral blood plasma was performed using an exclusive microRNA extraction kit. Then the cDNA synthesis of the target microRNA was performed and its concentration and purity were evaluated. The expression level of miR-206 was performed using the real-time PCR technique and the SYBER Green method, using U6 snRNA as an internal control. In order to analyze the amount of microRNA expression and the signifi cance of the patient sample, the t-test was used to compare the control sample. Also, Pearson correlation coeffi cient test was used to determine the relationship between the expression level of microRNAs. RESULTS: The results showed a positive correlation between miR-206 expression and coronary artery disease. While the average expression of 1 ± 0.18 in the control sample was increased to 8.76 according to the severity of involvement in the patient, the relative expression of miR-206 in the CAD + group was signifi cantly increased compared to the control (p < 0. 03). CONCLUSIONS: It appears that miR-206 can be considered as an indicator of coronary endothelial cell function. As such, it can be used as a biomarker for prognosis and in controlling the treatment for coronary artery disease (Tab. 2, Fig. 3, Ref. 20). Text in PDF www.elis.sk.

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Circulating microRNAs in Cardiovascular Diseases

Cited by 8 publications

References 47 publications

miR‑214 mediates vascular inflammation and apoptosis via PTEN expression

miR‑214 mediates vascular inflammation and apoptosis via PTEN expression

The Effects of Regular Exercise on Circulating Cardiovascular-related MicroRNAs

Relationship between microRNA-206 plasma levels with the severity of coronary artery conflicts in patients with coronary artery disease

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