Relationship between microRNA-206 plasma levels with the severity of coronary artery conflicts in patients with coronary artery disease

Abstract

OBJECTIVES: The aim of this study was to evaluate the possible association of miR-206 serum as an indicator of diagnosis in patients with coronary artery disease. METHODS: In this study, 100 patients with coronary artery disease who had angiography and vascular transplantation were selected and evaluated. Extraction of microRNAs from peripheral blood plasma was performed using an exclusive microRNA extraction kit. Then the cDNA synthesis of the target microRNA was performed and its concentration and purity were evaluated. The expression level of miR-206 was performed using the real-time PCR technique and the SYBER Green method, using U6 snRNA as an internal control. In order to analyze the amount of microRNA expression and the signifi cance of the patient sample, the t-test was used to compare the control sample. Also, Pearson correlation coeffi cient test was used to determine the relationship between the expression level of microRNAs. RESULTS: The results showed a positive correlation between miR-206 expression and coronary artery disease. While the average expression of 1 ± 0.18 in the control sample was increased to 8.76 according to the severity of involvement in the patient, the relative expression of miR-206 in the CAD + group was signifi cantly increased compared to the control (p < 0. 03). CONCLUSIONS: It appears that miR-206 can be considered as an indicator of coronary endothelial cell function. As such, it can be used as a biomarker for prognosis and in controlling the treatment for coronary artery disease (Tab. 2, Fig. 3, Ref. 20). Text in PDF www.elis.sk.

Non-Coding RNAs as Blood-Based Biomarkers in Cardiovascular Disease

“…Notwithstanding, the levels of circulating miRNAs are not only capable of indicating the presence of CAD lesion, but also can differentiate and correlate with CAD severity, as shown for miR-206 [54]. Patients with major blocked coronary arteries or with a higher number of blocked coronary arteries have higher levels of miR-206 when compared with patients with less severe lesions or healthy controls [54].…”

“…Notwithstanding, the levels of circulating miRNAs are not only capable of indicating the presence of CAD lesion, but also can differentiate and correlate with CAD severity, as shown for miR-206 [54]. Patients with major blocked coronary arteries or with a higher number of blocked coronary arteries have higher levels of miR-206 when compared with patients with less severe lesions or healthy controls [54]. As such, miR-206 is upregulated in individuals with three blocked coronary arteries when compared with individuals two blocked coronary arteries, and those showed enhanced levels of miR-206 when compared with individuals with only one blocked coronary artery.…”