Circulating MicroRNAs as Novel Biomarkers in Risk Assessment and Prognosis of Coronary Artery Disease

Vavassori, Chiara; Cipriani, Eleonora; Colombo, Gualtiero I.

doi:10.15420/ecr.2021.47

Cited by 10 publications

(7 citation statements)

References 73 publications

(135 reference statements)

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“…Asymptomatic course and progression make an early diagnosis of CAAD difficult. miRNAs play a role in the mechanism of atherosclerosis, and atherosclerotic coronary artery disease is characterized by specific signatures of miRNA in tissue and plasma assays [ 23 , 24 , 25 , 26 ]. However, scarce data concerning miRNAs involved in CAAD pathology prompted us to study five circulating miRNAs: MiR-125b-5p, miR-210-3p, miR-328-3p, miR-425-3p, and miR-483-5p, which we preselected and described in our former study [ 14 ] as potentially associated with CAAD.…”

Section: Discussionmentioning

confidence: 99%

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Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

Iwańczyk

Lehmann

Cieślewicz

et al. 2023

IJMS

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MicroRNAs (miRNAs) are currently investigated as crucial regulatory factors which may serve as a potential therapeutic target. Reports on the role of miRNA in patients with coronary artery aneurysmal disease (CAAD) are limited. The present analysis aims to confirm the differences in the expression of previously preselected miRNAs in larger study groups and evaluate their usefulness as potential markers of CAAD. The study cohort included 35 consecutive patients with CAAD (Group 1), and two groups of 35 patients matched Group 1 regarding sex and age from the overall cohort of 250 patients (Group 2 and Group 3). Group 2 included patients with angiographically documented coronary artery disease (CAD), while Group 3 enrolled patients with normal coronary arteries (NCA) assessed during coronary angiography. We applied the RT-qPCR method using the custom plates for the RT-qPCR array. We confirmed that the level of five preselected circulating miRNAs was different in patients with CAAD compared to Group 2 and Group 3. We found that miR-451a and miR-328 significantly improved the CAAD prediction. In conclusion, miR-451a is a significant marker of CAAD compared to patients with CAD. In turn, miR-328-3p is a significant marker of CAAD compared to patients with NCA.

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Section: Discussionmentioning

confidence: 99%

“…Since the presence of miRNAs in plasma was first mentioned in the literature, circulating miRNAs have been reported as a potential biomarker of heart failure and CAD [ 23 , 28 ]. In contrast to the numerous studies in CAD cohorts, the predictive value of miRNAs in CAAD is yet to be discovered.…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

Iwańczyk

Lehmann

Cieślewicz

et al. 2023

IJMS

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“…To date, multiple miRNAs related to chronic coronary syndromes have been identified, being proposed as biomarkers with potential clinical applications in diagnosis and prognosis. However, the miRNA expression shows wide variability in the literature [ 24 , 37 , 38 ]. To avoid this methodological problem, we will relate the different coronary patterns only to those circulating miRNAs showing consistent results in the different research works, and we will perform specific analyses to take into account the potential variability on miRNA levels between centres, since this is a multicentric study.…”

Section: Discussionmentioning

confidence: 99%

Study protocol for the epigenetic characterization of angor pectoris according to the affected coronary compartment: Global and comprehensive assessment of the relationship between invasive coronary physiology and microRNAs

et al. 2023

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Background MicroRNAs (miRNAs) are noncoding RNAs involved in post-transcriptional genetic regulation with a proposed role in intercellular communication. miRNAs are considered promising biomarkers in ischemic heart disease. Invasive physiological evaluation allows a precise assessment of each affected coronary compartment. Although some studies have associated the expression of circulating miRNAs with invasive physiological indexes, their global relationship with coronary compartments has not been assessed. Here, we will evaluate circulating miRNAs profiles according to the coronary pattern of the vascular compartment affectation. Study and design This is an investigator-initiated, multicentre, descriptive study to be conducted at three centres in Spain (NCT05374694). The study will include one hundred consecutive patients older than 18 years with chest pain of presumed coronary cause undergoing invasive physiological evaluation, including fractional flow reserve (FFR) and index of microvascular resistance (IMR). Patients will be initially classified into four groups, according to FFR and IMR: macrovascular and microvascular affectation (FFR≤0.80 / IMR≥25), isolated macrovascular affectation (FFR≤0.80 / IMR<25), isolated microvascular affectation (FFR>0.80 / IMR ≥25) and normal coronary indexes (FFR>0.80 / IMR<25). Patients with isolated microvascular affectation or normal indexes will also undergo the acetylcholine test and may be reclassified as a fifth group in the presence of spasm. A panel of miRNAs previously associated with molecular mechanisms linked to chronic coronary syndrome will be analysed using RT-qPCR. Conclusions The results of this study will identify miRNA profiles associated with patterns of coronary affectation and will contribute to a better understanding of the mechanistic pathways of coronary pathology.

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“…In addition, miRNAs play a significant role in lipid metabolism beyond cholesterol, and also in insulin signalling in the liver, thus contributing to the pathways that are often dysregulated in metabolic disorders, such as non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), which are closely linked to CAD 6 . MicroRNAs have been proposed as regulators and biomarkers of CAD pathogenesis but only few of these have been shown in several studies to be consistently up- or down-regulated in stable or acute CAD 7 . Variable results in miRNA studies are most probably due to factors like differences in miRNA detection methods, variable sample source as well as variable CAD criteria.…”

Section: Introductionmentioning

confidence: 99%

Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism

Alexanova

Raitoharju

Valtonen

et al. 2023

Sci Rep

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Metabolic dysfunction, partly driven by altered liver function, predisposes to coronary artery disease (CAD), but the role of liver in vulnerable atherosclerotic plaque development remains unclear. Here we produced hepatocyte-like cells (HLCs) from 27 induced pluripotent stem cell (iPSC) lines derived from 15 study subjects with stable CAD (n = 5), acute CAD (n = 5) or healthy controls (n = 5). We performed a miRNA microarray screening throughout the differentiation, as well as compared iPSC-HLCs miRNA profiles of the patient groups to identify miRNAs involved in the development of CAD. MicroRNA profile changed during differentiation and started to resemble that of the primary human hepatocytes. In the microarray, 35 and 87 miRNAs were statistically significantly deregulated in the acute and stable CAD patients, respectively, compared to controls. Down-regulation of miR-149-5p, -92a-3p and -221-3p, and up-regulation of miR-122-5p was verified in the stable CAD patients when compared to other groups. The predicted targets of deregulated miRNAs were enriched in pathways connected to insulin signalling, inflammation and lipid metabolism. The iPSC-HLCs derived from stable CAD patients with extensive lesions had a distinct genetic miRNA profile possibly linked to metabolic dysfunction, potentially explaining the susceptibility to developing CAD. The iPSC-HLCs from acute CAD patients with only the acute rupture in otherwise healthy coronaries did not present a distinct miRNA profile, suggesting that hepatic miRNAs do not explain susceptibility to plaque rupture.

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Circulating MicroRNAs as Novel Biomarkers in Risk Assessment and Prognosis of Coronary Artery Disease

Cited by 10 publications

References 73 publications

Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

Circulating miRNA-451a and miRNA-328-3p as Potential Markers of Coronary Artery Aneurysmal Disease

Study protocol for the epigenetic characterization of angor pectoris according to the affected coronary compartment: Global and comprehensive assessment of the relationship between invasive coronary physiology and microRNAs

Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolism

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