Circulating microRNAs and long non-coding RNAs in gastric cancer diagnosis: An update and review

Huang, Yakai; Yu, Jian-chun

doi:10.3748/wjg.v21.i34.9863

Cited by 116 publications

(80 citation statements)

References 219 publications

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“…However, MT1JP expression was not associated with factors such as gender, age, alcohol consumption, smoking, tumor differentiation, location, venous invasion, local invasion and distant metastasis. Non-invasive approach using circulating lncRNAs provides new alternatives for cancer detection and prognosis evaluation [15,17,18]. Thus, we also measured MT1JP expression in plasma samples from 34 GC patients and paired healthy donors.…”

Section: Correlation Of Mt1jp Expression With Clinicopathological Feamentioning

confidence: 99%

“…Mechanistically, lncRNAs may act as chromatin remodeler, transcriptional modulator, splicing regulator, posttranscriptional processor, enhancer, molecular decoys for miRNAs, or as a guide for protein-protein, protein-DNA and protein-RNA interaction [4,5,13,14]. Given the properties of disease-, tissue-and developmental-specificity, lncRNA may serve as a candidate biomarker for GC diagnosis [9,13,15]. Indeed, recent evidences have confirmed that several lncRNAs may not only provide significance for GC diagnosis, stage assessment and prognosis prediction, but also serve as promising targets for GC treatment [9,13,15].…”

Section: Introductionmentioning

confidence: 99%

“…Given the properties of disease-, tissue-and developmental-specificity, lncRNA may serve as a candidate biomarker for GC diagnosis [9,13,15]. Indeed, recent evidences have confirmed that several lncRNAs may not only provide significance for GC diagnosis, stage assessment and prognosis prediction, but also serve as promising targets for GC treatment [9,13,15].…”

Section: Introductionmentioning

confidence: 99%

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LncRNA MT1JP Suppresses Gastric Cancer Cell Proliferation and Migration Through MT1JP/MiR-214-3p/RUNX3 Axis

Xü

Zhang

Zou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Emerging evidence points towards an important role of long noncoding RNAs (lncRNAs) in the tumorigenesis and progression of gastric cancer (GC). MT1JP has recently been reported to be differentially expressed and act as a tumor suppressor in different tumors, with its mechanisms not fully understood in GC. Methods: RT-qPCR was used to detect the expression of MT1JP, miR-214-3p and RUNX3 in tumor tissues and cell lines of GC. The CCK-8 assay, colony formation, Transwell assay and wound healing assay were used to evaluate the proliferation, invasion and migration of GC cells, respectively. Bioinformatics analysis and luciferase reporter assay were performed to disclose the interaction between MT1JP, miR-214-3p and RUNX3. Western blot and immunofluorescence were applied to assess the downstream signaling of RUNX3. Results: MT1JP was found downregulated in GC tissues and cells. Low expression of MT1JP was significantly correlated with advanced TNM stage and lymphatic metastasis. The expression of plasma MT1JP was also found decreased in GC patients compared to healthy controls, with an area under the ROC curve (AUC) of 0.649 for diagnosis of GC. Gain-and loss-of-function of MT1JP revealed that MT1JP functioned as a ceRNA for miR-214-3p to facilitate RUNX3 expression and then upregulated p21 and Bim levels suppressing GC cell proliferation, invasion and migration, and promoting apoptosis. Furthermore, MT1JP overexpression suppressed tumor growth and inhibited the expression of miR-214-3p and proliferation antigen Ki-67, but increased the expression of RUNX3, p21 and Bim in vivo. Conclusions: Our results suggest a potential ceRNA regulatory network involving MT1JP regulates RUNX3 expression by competitively binding endogenous miR-214-3p in tumorigenesis and progression of GC. This mechanism may contribute to a better understanding of GC pathogenesis and provide potential therapeutic strategy for GC.

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Section: Correlation Of Mt1jp Expression With Clinicopathological Feamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA MT1JP Suppresses Gastric Cancer Cell Proliferation and Migration Through MT1JP/MiR-214-3p/RUNX3 Axis

Xü

Zhang

Zou

et al. 2018

Cell Physiol Biochem

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“…Moreover, besides the upregulated miRNAs, many circulating miRNAs are reduced in the blood of GC patients. miR-122 and miR-195 are the two mainly downregulated circulating miRNAs in GC patients, thus suggesting that both of them were potential diagnostic markers for GC screening [ 169 ].…”

Section: Gastric Cancermentioning

confidence: 99%

Noncoding RNAs in Cancer Diagnosis

Zeng

2016

Advances in Experimental Medicine and Biology

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The accuracy and efficiency of tumor treatment depends mainly on early and precise diagnosis. Although histopathology is always the gold standard for cancer diagnosis, noninvasive biomarkers represent an opportunity for early detection and molecular staging of cancer. Besides the classical tumor markers, noncoding RNAs (ncRNAs) emerge to be a novel category of biomarker for cancer diagnosis since the dysregulation of ncRNAs is closely associated with the development and progression of human cancers such as liver, lung, breast, gastric, and other kinds of cancers. In this chapter, we will summarize the different types of ncRNAs in the diagnosis of major human cancers. In addition, we will introduce the recent advances in the detection and applications of circulating serum or plasma ncRNAs and non-blood fluid ncRNAs because the noninvasive body fluid-based assays are easy to examine for cancer diagnosis and monitoring.

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“…Mature miRNA binds to the 3'-untranslated (UTR) region of its target mRNA, forming silencing complexes and inducing the degradation of target mRNA or inhibiting its translation (13,14). miRNAs are stable molecules that may be developed as biomarkers for various diseases and their levels are high in the peripheral blood (15). In addition, miRNAs are involved in almost all pathological and physiological processes in eukaryotic cells, including cancer, cardiovascular disease, Alzheimer's disease and inflammation (16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Role of microRNA‑26a in the diagnosis of lower extremity deep vein thrombosis in patients with bone trauma

2017

Exp Ther Med

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Abstract. The present study aimed to investigate the role and mechanism of action of microRNA (miR)-26a in deep vein thrombosis (DVT). Peripheral blood was collected from 45 patients with DVT and 40 healthy controls. Levels of miR-26a, chemokine C-C motif ligand (CCL)2 mRNA and CCL7 mRNA were detected using reverse transcription-quantitative polymerase chain reaction and the value of miR-26a in the clinical diagnosis of DVT was assessed using receiver operating characteristic curve analysis. The correlation of miR-26a with CCL2 and CCL7 levels was analyzed using Spearman's rank correlation. In addition, miR-26a and protein kinase C δ (PRKCD) were overexpressed in human umbilical vein endothelial cells (HUVECs) and PRKCD expression was knocked down by small interfering (si)RNA. Western blotting was conducted to detect the expression of PRKCD and p65. Furthermore, a dual-luciferase reporter gene assay was performed. The results of the current study demonstrated that the expression of miR-26a was significantly downregulated in the peripheral blood of patients with DVT compared with healthy controls (P<0.05) and negatively correlated with CCL2 and CCL7 levels (P<0.05). Furthermore, it was demonstrated that miR-26a markedly inhibited the expression of PRKCD, significantly decreased levels of CCL2 and CCL7 mRNA (P<0.05) and inhibited activation of the NF-κB signaling pathway. Overexpression of PRKCD in HUVECs inhibited the effects of miR-26a and markedly upregulated the phosphorylation of p65. The present study indicated that miR-26a directly targets PRKCD mRNA and that miR-26a may be a useful biomarker in the clinical diagnosis of DVT. Thus, the present findings suggest that miR-26a regulates the NF-κB signaling pathway by binding to PRKCD mRNA, inhibits the expression of CCL2 and CCL7 and reduces the risk of DVT.

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Circulating microRNAs and long non-coding RNAs in gastric cancer diagnosis: An update and review

Cited by 116 publications

References 219 publications

LncRNA MT1JP Suppresses Gastric Cancer Cell Proliferation and Migration Through MT1JP/MiR-214-3p/RUNX3 Axis

LncRNA MT1JP Suppresses Gastric Cancer Cell Proliferation and Migration Through MT1JP/MiR-214-3p/RUNX3 Axis

Noncoding RNAs in Cancer Diagnosis

Role of microRNA‑26a in the diagnosis of lower extremity deep vein thrombosis in patients with bone trauma

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