Circulating microRNA signature of steroid‐induced osteonecrosis of the femoral head

Abstract

ObjectivesSteroid‐induced osteonecrosis of the femoral head (ONFH) is a common orthopaedic disease of which early detection remains clinically challenging. Accumulating evidences indicated that circulating microRNAs (miRNAs) plays vital roles in the development of several bone diseases. However, the association between circulating miRNAs and steroid‐induced ONFH remains elusive.Materials and methodsmiRNA microarray was performed to identify the differentially abundant miRNAs in the serums of systemic lupus erythematosus (SLE) patients with steroid‐induced ONFH as compared with SLE control and healthy control group. We predicted the potential functions of these differentially abundant miRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and reconstructed the regulatory networks of miRNA‐mRNA interactions.ResultsOur data indicated that there were 11 differentially abundant miRNAs (2 upregulated and 9 downregulated) between SLE‐ONFH group and healthy control group and 42 differentially abundant miRNAs (14 upregulated and 28 downregulated) between SLE‐ONFH group and SLE control group. We also predicted the potential functions of these differentially abundant miRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and reconstructed the regulatory networks of miRNA‐mRNA interactions.ConclusionsThese findings corroborated the idea that circulating miRNAs play significant roles in the development of ONFH and may serve as diagnostic markers and therapeutic targets.

Changed cellular functions and aberrantly expressed miRNAs and circRNAs in bone marrow stem�cells in osteonecrosis of the femoral head

“…Thus, it was speculated that the dE circRNAs or miRNAs associated with blood loss could also be correlated with ONFH. Third, the dE circRNAs and miRNAs might also result from the autoimmune diseases such as SLE instead of ONFH, which has been demonstrated in previous studies (44)(45)(46). It is true that SLE itself could lead to DE miRNAs and circRNAs.…”

Noncoding RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

“…Moreover, RT-qPCR confirmed a significant increase in the level of miR-10a-5p and a decrease in the level of miR-423-59 in the patient samples. Similarly, Li et al [16] found 11 differentially expressed miRNAs (two upregulated and nine downregulated) between the ONFH group and healthy control group. Furthermore, 42 differentially expressed miRNAs (14 upregulated and 28 downregulated) were found between the ONFH group and a control group comprising patients with systemic lupus erythematosus (SLE).…”

Expression Profile Analysis of Differentially Expressed Circular RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

“…Normal SONFH Disease Markers patients and control individuals. Their data indicated that circulating miRNAs in the serum might play notable roles in the development of SONFH and act as diagnostic biomarkers [15]. Kao et al compared miRNAs in peripheral blood by microarray and PCR, implying the possibility of using miRNAs as novel diagnostic or therapeutic targets [16].…”

“…Our study found 229 upregulated circRNAs and 116 downregulated cir-cRNAs. We further selected eight circRNAs for preliminary qRT-PCR validation based on a combination of previous studies and the circRNA-miRNA prediction network (Table 2) [15,[26][27][28][29]. The results indicated that the RNAseq data was reliable and worthy of further study.…”