Circulating lipid peroxidation, plasma and erythrocyte antioxidant status in patients with rheumatoid arthritis

Abstract

The aim of the study was to investigate the levels of lipid peroxidation, and both plasma and erythrocyte antioxidant states in patients with rheumatoid arthritis. The population consisted of 60 subjects divided into two groups, 30 subjects had evidence of rheumatoid arthritis and age and sex matched healthy subjects were studied as controls. The level of plasma and erythrocyte thiobarbituric acid reactive substances (TBARS) was markedly increased in both the rheumatoid arthritis patients when compared to control subjects. The activities of plasma and erythrocyte antioxidants were significantly decreased in rheumatoid arthritis. C reactive protein, rheumatoid factor and antistreptolysin-O were significantly higher in rheumatoid arthritis patients than in healthy subjects. In conclusion, on the basis of enhanced lipid peroxidation in rheumatoid arthritis patients with concomitant failure of both the plasma, and erythrocyte antioxidants defense mechanism. These results are consistent with the underlying hypothesis that there is an imbalance between reactive oxygen species production and the antioxidant defense system in inflammatory rheumatoid arthritis disease.

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“…We have also demonstrated that Lipid peroxidation reflected by MDA levels significantly increased in RA patients compared to normal healthy subjects; these results are in agreement with many studies in plasma 6,20 , in erythrocytes 4 and in synovial fluid 13,15 . As a whole, the negative correlation deduced between MDA and the activities of GPx and CAT 13 confirm the pro-oxidant effect of MDA in RA onset.…”

“…In fact, many studies have shown that the antioxidant system was impaired in patients [4][5] and oxidative stress markers were increased in patients with RA 6,7 . To the best of our knowledge, this is the first study performed in Tunisian patients with RA and the largest RA study population actually analysed for oxidative stress parameter correlations.…”

Coenzyme Q10 suppresses Th17 cells and osteoclast differentiation and ameliorates experimental autoimmune arthritis mice

“…It has been well established that free radicals and reactive oxygen species are involved with the pathogenesis of RA [7,8]. There is also evidence showing increased levels of oxidative stress among RA patients when compared to controls [9] and the balance between reactive oxygen species production and the antioxidant defense system is disproportionate in RA [10].…”

Elucidation of molecular mechanisms underlying the protective effects of thymoquinone against rheumatoid arthritis

“…Other key modulators of joint inflammation in RA are reactive oxygen species (ROS) and lipid peroxidation (LPO) end‐products. Large amounts of ROS and LPO products have been detected in the serum and synovial fluid of inflamed rheumatoid joints, and this production can be induced by cytokine stimulation [Remans et al, 2005; Pasupathi et al, 2009]. LPO products, mainly 4‐hydroxynonenal (HNE), form adducts with proteins and make them highly immunogenic.…”