Circulating levels of miR-150 are associated with poorer outcomes of A/H1N1 infection

Morán, Juan; Ramírez-Martínez, Gustavo; Jiménez-Álvarez, Luis Armando; Cruz, Alfredo Torres; Pérez-Patrigeón, Santiago; Hidalgo, Alfredo; Orozco, Lorena; Martínez, Angélica; Padilla-Noriega, Luis; Ávila‐Moreno, Federico; Cabello, Carlos; Granados, Julio; Ortiz‐Quintero, Blanca; Ramírez‐Venegas, Alejandra; Ruíz-Palacios, Guillermo M.; Zlotnik, Albert; Merino, Enrique; Zúñiga, Joaquı́n

doi:10.1016/j.yexmp.2015.07.001

Cited by 34 publications

(29 citation statements)

References 40 publications

(41 reference statements)

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“…related pneumonia, e.g., miR-150 was significantly up-regulated in serum, whereas the expression of miR-210, miR-126 and miR-222 was decreased, albeit the small sample size precludes definitive conclusions 4 .…”

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confidence: 99%

Transcriptional analysis identifies potential biomarkers and molecular regulators in pneumonia and COPD exacerbation

Bertrams

Griss

Han

et al. 2020

Sci Rep

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Lower respiratory infections, such as community-acquired pneumonia (cAp), and chronic obstructive pulmonary disease (copD) rank among the most frequent causes of death worldwide. improved diagnostics and profound pathophysiological insights are urgent clinical needs. in our cohort, we analysed transcriptional networks of peripheral blood mononuclear cells (pBMcs) to identify central regulators and potential biomarkers. We investigated the mRnA-and miRnA-transcriptome of pBMcs of healthy subjects and patients suffering from CAP or AECOPD by microarray and Taqman Low Density Array. Genes that correlated with PBMC composition were eliminated, and remaining differentially expressed genes were grouped into modules. One selected module (120 genes) was particularly suitable to discriminate AECOPD and CAP and most notably contained a subset of five biologically relevant mRNAs that differentiated between CAP and AECOPD with an AUC of 86.1%. Likewise, we identified several microRNAs, e.g. miR-545-3p and miR-519c-3p, which separated AECOPD and CAP. We furthermore retrieved an integrated network of differentially regulated mRNAs and microRNAs and identified HNF4A, MCC and MUC1 as central network regulators or most important discriminatory markers. in summary, transcriptional analysis retrieved potential biomarkers and central molecular features of cAp and AecopD. Community acquired pneumonia (CAP) is clinically defined by a sudden onset of severe illness that is accompanied by signs of lower respiratory tract infection, fever, cough and dyspnoea 1. When left untreated, severe secondary effects such as organ damage and occurrence of bacteria in the blood (bacteremia) can ensue. While subject to variance due to region, season and population characteristics, the incidence of CAP is estimated to lie between 1.5 and 14 cases per 1,000 persons per year, with children under 5 years of age and the elderly of more than 65 years being most strongly affected 2. Immunocompromised persons also bear a higher risk of CAP contraction. The leading underlying cause of CAP is infection with the gram-positive bacterium Streptococcus pneumoniae, accounting for 30-35% of CAP cases worldwide 3. The initial colonization of the nasopharynx and the upper respiratory tract often remains asymptomatic. Aspiration into the alveoli can cause severe respiratory or systemic disease, depending on the host immune status and the pneumococcal serotype 1. As CAP is a multifaceted disease with a host of potential causative agents, the robust identification of microR-NAs that are functionally involved in pneumonia depends on the pathogen. In severe Influenza A Virus (H1N1)

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confidence: 99%

Transcriptional analysis identifies potential biomarkers and molecular regulators in pneumonia and COPD exacerbation

Bertrams

Griss

Han

et al. 2020

Sci Rep

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“…Further, circulating miR-150 levels have been reported to correlate with pro-inflammatory cytokine-induced fever. A significant increase in the circulating miR-150 levels has been reported to associate with fatal outcome in A/H1N1 patients compared to patients that survived the febrile infection [42]. Upregulation of miR-150 in SD patients at the time of admission, within 4 days from fever onset suggests a role similar to other febrile illnesses.…”

Section: Discussionmentioning

confidence: 96%

Differential expression of microRNA, miR-150 and enhancer of zeste homolog 2 (EZH2) in peripheral blood cells as early prognostic markers of severe forms of dengue

et al. 2020

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Background: Dengue presents a wide clinical spectrum. Most patients recover following a self-limiting non-severe clinical course. A small proportion of patients progress to severe disease, mostly characterized by plasma leakage with or without hemorrhage. Early symptoms of severe dengue (SD) are similar to those of non-severe dengue fever (DF). Severe symptoms manifest after 3-5 days of fever, which can be life threatening due to lack of proper medications and inability to distinguish severe cases during the early stages. Early prediction of SD in patients with no warning signs who may later develop severe infection is very important for proper disease management to alleviate related complications and mortality. microRNA are small non-coding RNA molecules that regulate post-transcriptional gene expression. Due to the remarkable stability and the role of microRNA in gene expression, altered expression of microRNA was evaluated to explore clinically relevant prognostic markers of severe dengue. Methods: The relative expression of microRNA hsa-let-7e (let-7e), hsa-miR-30b-5p (miR-30b), hsa-miR-30e-3p (miR-30e), hsa-miR-33a (miR-33a), and hsa-miR-150-5p (miR-150) and several putative target genes in peripheral blood cells (PBC) collected from 20 DF and 20 SD positive patients within 4 days from fever onset was evaluated by quantitative reverse transcription PCR (qRT-PCR).Results: miR-150 showed significant (P < 0.01) up regulation in PBC of SD patients compared to DF patients during the acute phase of infection. Expression of enhancer of zeste homolog 2 (EZH2) was significantly (P < 0.01) down regulated indicating that genes involved in epigenetic regulation are also differentially expressed in SD patients during the early stage of infection.Conclusions: Differential expression of microRNA miR-150 and the putative target gene EZH2 may serve as reliable biomarkers of disease severity during early stages of dengue infection.

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“…Patients with severe A/H1N1 disease exhibited a significant over-expression of circulating MIR150 than patients with milder disease, suggesting that the up-regulation of MIR150 is engaged with poorer outcomes of A/H1N1 infection (Morán et al, 2015).…”

Section: Influenzamentioning

confidence: 99%

MIR150 (microRNA 150)

Arjmand¹

2018

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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MicroRNAs (miRNAs) are a class of small regulatory RNAs (20-23 nucleotides long) that have been found to play a critical role in the wide spectrum of biological processes. These small endogenous noncoding RNAs are able to negatively regulate gene expression by pairing with the 3'untranslated region (3'-UTR) of their target mRNA. miR-150 is a short and single-stranded miRNA, encoded by a gene located at 19q13.33 in Homo sapiens, which is processed to two mature forms (miR-150-5p and miR-150-3p). It has proven to be a pivotal miRNA engaged in the development of hematologic malignancies, normal haematopoiesis, differentiation of B and T cells, and a number of tumours including breast, osteosarcoma, lung, gastric cancer and etc.

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Circulating levels of miR-150 are associated with poorer outcomes of A/H1N1 infection

Cited by 34 publications

References 40 publications

Transcriptional analysis identifies potential biomarkers and molecular regulators in pneumonia and COPD exacerbation

Transcriptional analysis identifies potential biomarkers and molecular regulators in pneumonia and COPD exacerbation

Differential expression of microRNA, miR-150 and enhancer of zeste homolog 2 (EZH2) in peripheral blood cells as early prognostic markers of severe forms of dengue

MIR150 (microRNA 150)

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