Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight

Circulating chromosome 19 miRNA cluster microRNAs in pregnant women with severe pre‐eclampsia

“…Furthermore, in the developing placenta, C19MC miRNAs might participate in the trophoblastic proliferation necessary to constitute a large placenta, and in the deep invasion of the maternal decidua necessary to establish the correct blood flow to fulfill fetal needs. A previous study reported that circulating cell‐free C19MC miRNAs (miR‐515‐3p, ‐517a, ‐517c and ‐518b) were significantly associated with placental weight . Despite the lower weight of placenta tissues in the PE group compared with the NP group (Table ), maternal plasma concentration of C19MC miRNAs was significantly increased in pregnant women with sPE than in NP women.…”

Circulating levels of maternal plasma cell‐free miR‐21 are associated with maternal body mass index and neonatal birth weight

“…Our previous study showed that circulating levels of cell-free placenta-specific miRNAs were associated with PW but not with BMI or BW. 10 In the current study, circulating cell-free miR-21 levels, which are expressed in maternal, fetal, and placental tissues, were associated with BMI and BW but not with PW. All of the cell-free placentaspecific miRNAs in maternal plasma are of placental origin.…”

“…Therefore, similar to the association between cell‐free DNA and maternal BMI, increased plasma cell‐free miRNA levels may reflect increased levels of necrosis of adipocytes and stromal vascular apoptosis. Our previous study showed that circulating levels of cell‐free placenta‐specific miRNAs were associated with PW but not with BMI or BW . In the current study, circulating cell‐free miR‐21 levels, which are expressed in maternal, fetal, and placental tissues, were associated with BMI and BW but not with PW.…”

“…Each sample and each calibration dilution were analyzed in triplicate. Each assay could detect down to 300 RNA copies/mL [9,10]. 5,6 Every batch of amplifications included three water blanks as negative controls for each of the reverse transcription and PCR steps.…”

Circulating Organ-Specific MicroRNAs Serve as Biomarkers in Organ-Specific Diseases: Implications for Organ Allo- and Xeno-Transplantation

“…MiRNA expression profiles, including cell/tissue/organ-specific miRNAs, indicate different diseases, such as cancers [14]. Profiles of miRNA in the body fluids, also called circulating miRNA or cell-free miRNA, serve as non-invasive biomarkers for physiological and pathological changes, such as cancers [15], organ injury [16], diabetes [15], and even pregnancy [17,18]. …”