Circulating Insulin-like Growth Factor I Regulates Its Receptor in the Brain of Male Mice

Trueba-Saiz, Ángel; Fernández, Adolfo; Nishijima, Takeshi; Mecha, Míriam; Santi, Andrea; Munive, Victor; Torres‐Alemán, Ignacio

doi:10.1210/en.2016-1468

Cited by 25 publications

(27 citation statements)

References 25 publications

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“…It has a synergistic effect with PDGF which enhances the regeneration rate (C. Centeno et al, 2017). In response to neurodegeneration and brain damage, it also exhibits neuroprotective activities (Trueba-Saiz et al, 2017). Additionally, to increase remyelination, it increases the fatty acids synthesis (C. Centeno et al, 2017).…”

Section: Igfmentioning

confidence: 99%

Novel therapeutic approaches in utilizing platelet lysate in regenerative medicine: Are we ready for clinical use?

Zamani

Yaghoubi

Movassaghpour

et al. 2019

Journal Cellular Physiology

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Hemoderivative materials are used to treat different diseases. These derivatives include platelet‐rich plasma, serum, platelet gel, and platelet lysate (PL). Among them, PL contains more growth factors than the others and its production is inexpensive and easy. PL is one of the proper sources of platelet release factors. It is used in cells growth and proliferation and is a good alternative to fetal bovine serum. In recent years, the clinical use of PL has gained more appeal by scientists. PL is a solution saturated by growth factors, proteins, cytokines, and chemokines and is administered to treat different diseases such as wound healing, bone regeneration, alopecia, oral mucositis, radicular pain, osteoarthritis, and ocular diseases. In addition, it can be used in cell culture for cell therapy and tissue transplantation purposes. Platelet‐derived growth factor, fibroblast growth factor, insulin‐like growth factor, transforming growth factor β, and vascular endothelial growth factor are key PL growth factors playing a major role in cell proliferation, wound healing, and angiogenesis. In this paper, we scrutinized recent advances in using PL and PL‐derived growth factors to treat diseases and in regenerative medicine, and the ability to replace PL with other hemoderivative materials.

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Section: Igfmentioning

confidence: 99%

Novel therapeutic approaches in utilizing platelet lysate in regenerative medicine: Are we ready for clinical use?

Zamani

Yaghoubi

Movassaghpour

et al. 2019

Journal Cellular Physiology

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show abstract

“…For microglial cultures, supernatants were centrifuged (1000 rpm/5 min), re-suspended in DMEM/F12 (Life Technologies) + FBS (Gibco, USA), HS and penicillin/streptomycin solution. Cells were seeded at 12.5-10 4 cells/cm 2 in a multi-well coated with poly-L-lysine [29], and cultured for 2 days. Cells were then changed to DMEM/F12 for 3 hours until Aβ uptake was carried out (see below).…”

Section: Methodsmentioning

confidence: 99%

“…When 80% con uency was reached, astrocytes were cultured for 3 hours with DMEM/F12 before the different assays were initiated (see below). Endothelial cell cultures were performed as described [29]. Brie y, dissection was performed on ice and cortices were cut into small pieces (1 mm 3 ), digested in a mixture of collagenase/dispase (270 U collagenase/ml, 10% dispase) and DNAse (10 U/ml) in DMEM for 1.5 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Circulating insulin-like growth factor I is involved in the effect of diet on peripheral amyloid β clearance

Herrero-Labrador

Trueba-Saiz

Martinez-Rachadell

et al. 2020

Preprint

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BackgroundObesity is a risk factor for Alzheimer´s disease (AD), but underlying mechanisms are not clear.MethodsWe analyzed peripheral clearance of amyloid β (Aβ) in overweight mice because its systemic elimination may impact on brain Aβ load, a major landmark of AD pathology. We also analyzed whether circulating insulin-like growth factor I (IGF-I) intervenes in the effects of overweight as this growth factor modulates brain Aβ clearance, and is increased in serum of overweight mice. Results Overweight mice showed increased peripheral Aβ clearance by the liver, the major site of elimination of systemic Aβ, but unaltered brain Aβ levels. We also found that Aβ clearance by hepatocytes is stimulated by IGF-I, and that mice with low serum IGF-I levels show reduced peripheral Aβ clearance and unchanged brain Aβ levels. In the brain, IGF-I favored association of its receptor (IGF-IR) with Aβ precursor protein (APP), and at the same time stimulated non-amyloidogenic processing of APP in astrocytes, as indicated by an increased sAPPα/sAPPβ ratio after IGF-I treatment. Since serum IGF-I enters into the brain in an activity-dependent manner, we analyzed in overweight mice the effect of brain activation by environmental enrichment (EE) on brain IGF-IR phosphorylation and its association to APP, as a readout of IGF-I activity. After EE, significantly reduced brain IGF-IR phosphorylation and APP/IGF-IR association was found in overweight mice as compared to lean controls. Conclusions Collectively, these results indicate that diet influences peripheral clearance of Aβ without affecting brain Aβ load. Increased serum IGF-I likely contributes to enhanced peripheral Aβ clearance in overweight mice, without affecting brain Aβ clearance probably because its brain entrance is reduced.

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“…For microglial cultures, supernatants were centrifuged (1000 rpm/5 min), resuspended in DMEM/F12 (Life Technologies)+FBS (Gibco, USA), HS and penicillin/streptomycin solution. Cells were seeded at 12.5-10 4 cells/cm 2 in a multi-well coated with poly-L-lysine(65), and cultured for 2 days. Cells were then changed to DMEM/F12 for 3 hours until Aβ uptake was carried out (see below).…”

mentioning

confidence: 99%

“…When 80% confluency was reached, astrocytes were cultured for 3 hours with DMEM/F12 before the different assays were initiated (see below). Endothelial cell cultures were performed as described(65).Briefly, dissection was performed on ice and cortices were cut into small pieces (1mm 3 ), digested in a mixture of collagenase/dispase (270 U collagenase/ml, 10% dispase) and DNAse (10 U/ml) in DMEM for 1.5 h at 37°C. The cell pellet was separated by centrifugation in 20% bovine serum albumin/DMEM (1000g, 5 min).…”

mentioning

confidence: 99%

Diet Influences Peripheral Amyloid Β Metabolism: A Role for Circulating Insulin-Like Growth Factor I

Raquel

Trueba-Saiz

Martinez-Rachadell

et al. 2020

Preprint

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AbstractObesity is a risk factor for Alzheimer’s disease (AD), but underlying mechanisms are not clear. We analyzed peripheral clearance of amyloid β (Aβ) in overweight mice because its systemic elimination may impact on brain Aβ load, a major landmark of AD pathology. Overweight mice showed increased peripheral Aβ clearance by the liver, the major site of elimination of systemic Aβ, but unaltered brain Aβ levels. Since circulating insulin-like growth factor I (IGF-I) modulates brain Aβ clearance, and is increased in serum of overweight mice, we determined whether it affects peripheral Aβ clearance. We found that Aβ uptake by hepatocytes is stimulated by IGF-I. Moreover, mice with low serum IGF-I levels show reduced peripheral Aβ clearance. In the brain, IGF-I favored association of its receptor (IGF-IR) with Aβ precursor protein (APP), and at the same time stimulated non-amyloidogenic processing of APP in astrocytes, as indicated by an increased sAPPα/sAPPβ ratio after IGF-I treatment. Since serum IGF-I enters into the brain in an activity-dependent manner, we analyzed in overweight mice the effect of brain activation by environmental enrichment (EE) on brain IGF-IR phosphorylation and its association to APP, as a readout of IGF-I activity. After EE, significantly less activation of brain IGF-IR phosphorylation and APP/IGF-IR association was found in overweight mice as compared to lean controls. Collectively, these results indicate that diet influences peripheral clearance of Aβ without affecting brain Aβ load. Increased serum IGF-I likely contributes to enhanced peripheral Aβ clearance in overweight mice, without affecting brain Aβ clearance probably because its brain entrance is reduced.

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Circulating Insulin-like Growth Factor I Regulates Its Receptor in the Brain of Male Mice

Cited by 25 publications

References 25 publications

Novel therapeutic approaches in utilizing platelet lysate in regenerative medicine: Are we ready for clinical use?

Novel therapeutic approaches in utilizing platelet lysate in regenerative medicine: Are we ready for clinical use?

Circulating insulin-like growth factor I is involved in the effect of diet on peripheral amyloid β clearance

Diet Influences Peripheral Amyloid Β Metabolism: A Role for Circulating Insulin-Like Growth Factor I

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