Circulating immune complexes in amoebiasis

Nuti, M.; D’Amelio, Raffaele; Seminara, R.; Palmisano, Lucia; Aiuti, F

doi:10.1016/0035-9203(82)90168-7

Cited by 2 publications

(2 citation statements)

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“…Recently, increased serum immune complexes have been used as a tool for the diagnosis of patients with amoebic liver abscess and chronic cyst passers. CIC have been reported to be higher in patients with active amoebiasis, and surprisingly, in cyst passers the prevalence and mean number of CIC were even higher (13). In another study, the presence of CIC was documented in 58% of amoebic liver abscess cases (14).…”

Section: Discussionmentioning

confidence: 92%

“…Circulating immune complexes (CIC) have been documented in many parasitic infections, such as malaria (5), trypanosomiasis (3), schistosomiasis (11), onchocerciasis (12), and toxoplasmosis (17). Increased soluble immune complexes have also been documented in amoebic liver abscess patients and chronic cyst passers (13)(14)(15). The specificity of these complexes, in terms of diagnostic applicability by demonstrating the presence of amoebic antigen in CIC, may discriminate present from past amoebic infection.…”

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confidence: 99%

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Specific circulating immune complexes in amoebic liver abscess

Vinayak¹,

Purnima²,

Singh³

et al. 1986

J Clin Microbiol

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A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% (14 of 15 confirmed cases) had indications of amoebic antigen in CIC. In 57% of the suspected cases of amoebic liver abscess, amoebic antigen could be detected in CIC. The specificity of the technique for the demonstration of specific amoebic antigen is indicated by the undetectable levels of amoebic antigen in CIC in serum samples from nine cases of nonamoebic hepatic diseases and 10 apparently healthy subjects. It is thus believed that the specific diagnosis of an individual case of amoebic liver abscess can be made by demonstrating specific amoebic antigen in CIC.

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

Specific circulating immune complexes in amoebic liver abscess

Vinayak¹,

Purnima²,

Singh³

et al. 1986

J Clin Microbiol

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Uses and limitations in the demonstration of specific circulating immune complexes in patients with amoebiasis

Vinayak¹,

Shandil²,

Bansal

et al. 1990

Journal of Medical Microbiology

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Summary.A micro-enzyme linked immuaosorbent assay (micro-ELISA) has been evaluated as a diagnostic test to detect amoebic antigen in polyethylene glycol (PEG) precipitated circulating immune complexes (CIC) in sera from patients with amoebiasis. The immune complexes were captured on rabbit anti-amoebic IgGcoated wells of microtitration plates and the complexed antigen was detected by enzyme linked antihuman immunoglobulins. A titre of > 160 for the immune complexes was considered to be of clinical significance. The immunoassay detected amoebic, antigen-specific CIC in 35 (94.5%) of 37 patients with confirmed amoebic liver abscess. Twenty (55.5%) of 36 clinically suspected cases of amoebic liver abscess had amoebic antigen-specific CIC and responded favourably to anti-amoebic chemotherapy. Only two (20%) of 10 cases of non-dysenteric symptomatic intestinal amoebic infection had amoebic antigen-specific CIC. One (10%) of 10 patients with non-amoebic intestinal disorders also had amoebic antigen in CIC. However, none of 15 cases of non-amoebic hepatic disorders that included hydatid disease, metastatic adenocarcinoma, hepatocellular carcinoma, cholecystitis and choledocal cyst, 13 cases of rheumatoid arthritis and 25 apparently healthy subjects had amoebic antigen in CIC. The levels of the amoebic antigen-specific CIC did not correlate (p>O-O5) with either the number of abscess(es) or lobe(s) of the liver involved. However, the levels of antigen-specific CIC were higher (p < 0.01) in patients with a liver size of more than 5 cm below the right costal margin. Antigen-specific CIC levels tended to decline or disappear during 3-6 months following completion of therapy. In spite of the limitations for diagnosing symptomatic intestinal amoebic disease, the demonstration of specific CIC is recommended as an immuno-diagnostic procedure for patients with suspected amoebic liver abscess.

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Circulating immune complexes in amoebiasis

Cited by 2 publications

References 1 publication

Specific circulating immune complexes in amoebic liver abscess

Specific circulating immune complexes in amoebic liver abscess

Uses and limitations in the demonstration of specific circulating immune complexes in patients with amoebiasis

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