Circulating Immune Complexes After Kidney Transplantation

Palosuo, Timo; Kano, Kyoichi; Anthone, Sidney; Gerbasi, Joseph R.; Milgrom, Felix

doi:10.1097/00007890-197604000-00007

Cited by 25 publications

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“…does not define the relationship of such complexes to the rejection phenomena (5 The histopathologic changes were assessed semiquantitatively by two observers independently, and without knowledge of the results of the assay for circulating immune complexes. Particular attention was paid to the presence of fibrin deposition in the microvasculature.…”

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confidence: 99%

Circulating Immune Complexes after Renal Transplantation

Ooi¹,

Ooi²,

Vallota³

et al. 1977

J. Clin. Invest.

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A B S T R A C T To assess the role ofcirculating immune complexes in the pathogenesis of acute rejection, sera were measured for such complexes by the 125I-Clq binding assay in 45 normal subjects, 24 allografted patients undergoing acute rejection, and in 11 allografted patients in a quiescent phase. Increased Clqbinding activity (Clq-BA) was detected in 14 patients with acute rejection, 9 of whom had renal biopsies showing fibrin deposition in the vasculature together with cellular infiltrates in the tubulo-interstitial structures; renal histology was not available in the other 5 patients. The other 10 patients with acute rejection, whose biopsies showed only cellular infiltrates, and the 11 patients in a quiescent phase posttransplantation did not have increased levels of serum Clq-BA.Of the group with increased serum Clq-BA, serial studies in eight patients showed a correlation between increased serum Clq-BA and the occurrence of rejection; with reversal by therapy, serum Clq-BA returned to within normal levels. Complexes from six patients were analyzed by sucrose density gradient ultracentrifugation to have sedimentation coefficients ranging from 15S to 18.4S. After acid dissociation and analysis by double-diffusion techniques, Clq-reactive complexes were shown to contain IgG. Immunofluorescent studies done in five renal biopsies from this group revealed granular deposits of immunoThis work has been previously published in abstract form: 1976. Kidney Int. 10: 608. (Abstr.)

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confidence: 99%

Circulating Immune Complexes after Renal Transplantation

Ooi¹,

Ooi²,

Vallota³

et al. 1977

J. Clin. Invest.

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“…Group) IV: Acute niephritis (patietnts [19][20][21][22][23][24]. Of the patients in this grouip (four males and two f'emales), four (nos.…”

Section: Detection Of Cytotoxic Anti-lymphocyte Antibodymentioning

confidence: 99%

“…Of the patients in this grouip (four males and two f'emales), four (nos. [19][20][21][22] had typical acute poststreptococcal glomeruilonlephritis with proven 8-hemolytic streptococcal pharyngitis or skinl inif'ection, hvpertension, hematuria and erythrocyte casts, decreased serumiii C3 levels, increasing or high antistreptolvsin 0 titer, proliferative glomerular changes and granular deposits of Ig and (or) C3, and they recovered with conservative management. The other two patients (nos.…”

Section: Detection Of Cytotoxic Anti-lymphocyte Antibodymentioning

confidence: 99%

“…Initial success with assays for circulating immune complexes in systemic immune complex diseases, such as systemic lupus erythematosus (14)(15)(16), has spurred interest in applying these tests to the study of serum from patients with primary immune complex glomerulonephritis. Immune complexes have been detected in sera from some but not all patients with presumed immune complex glomerulonephritis by the microcomplement consumption test (17,18), the platelet aggregation assay (19), the radiolabeled Clq binding assay (18,(20)(21)(22)(23), the macrophage uptake assay (24), the Clq deviation test (25), the bovine conglutinin assay (26), and the Raji cell radioimmune assay (*IRCA) (18). 1 The solid phase Clq assay (ClqSPA) (27) and the *IRCA (28) were employed in the present study to provide quantitative information regarding the frequency of detection of circulating immune complexes in very well-characterized individuals with glomerulonephritis.…”

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confidence: 99%

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Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.

Tung¹,

Woodroffe²,

Ahlin³

et al. 1978

J. Clin. Invest.

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“…Quite a popular reaction which has been recently used for detection of circulating im mune complexes is platelet aggregation test (PA) described by Penttinen and Myllyld and their co-workers [20][21][22][23], In our stud ies employing this test [24], we were mostly interested in detection of immune complex es in sera of patients after renal transplanta tion. We modified the procedure in such a way that 50 ml of peripheral human blood supplied enough platelets for an average test.…”

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Comparison of Various Procedures for the Detection of Antigen-Antibody Complexes

Milgrom¹,

Kano²

1978

Int Arch Allergy Immunol

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The described test of inhibition of AA activity proved to be a procedure of exquisite sensitivity and specificity for detection of immune complexes. This could have been expected since AA is an antibody specifically combining with IgG antibody that suffered molecular transformation in the reaction with its corresponding antigen. Complexes formed at or close to the equivalence zone were most active in inhibiting AA, whereas those at extreme antigen excess did not inhibit it at all. Inhibition of AA was capable of detecting circulating immune complexes as well as immune complexes deposited in the tissues. It was also shown that by using this procedure, identification of the antigen participating in immune complex formation is feasible since excess of the specific antigen converts inhibitory complexes into inactive complexes.

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Circulating Immune Complexes After Kidney Transplantation

Cited by 25 publications

References 0 publications

Circulating Immune Complexes after Renal Transplantation

Circulating Immune Complexes after Renal Transplantation

Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.

Comparison of Various Procedures for the Detection of Antigen-Antibody Complexes

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