Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.

Circulating Immune Complexes in Lyme Arthritis

“…Among these, one reacted strongly and three less so when the other assays were negative. Although the Raji cell and the Clq solid phase assays are about equally sensitive for detecting aggregated IgG (23), we have identified similar discrepancies between the two in patients with disseminated gonococcemia (12) and with glomerulonephritis (23). These discrepancies may reflect differences in conformation and (or) size among immune complexes, that favor reactivity in one or another system.…”

“…This advantage is offset to a certain extent by the nonspecific reactivity of Clq with such substances as C-reactive protein, DNA, and endotoxin (20)(21)(22). In contrast, the Clq solid phase assay is theoretically specific for immune complexes containing immunoglobulin that reacts with both Clq and staphylococcal protein A-mainly IgG1, and IgG2 (23). Although this assay might fail to detect complexes composed of IgM, IgA, or IgG3, it avoids the possible influence of interfering substances.…”

Modification of glomerular immune complex deposition in mice by activation of the reticuloendothelial system.

“…Immune complexes have been detected in the sera of patients with diffuse proliferative gloimierulonephritis, lupus nephritis, membranoproliferative glomerulonephritis, and other forms of nephritis (22)(23)(24). Studies of reticuloendothelial function in some ofthese forms of human renal disease have also shown impaired splenic clearance ofimmune complexes in these patients (25,26).…”

Glomerulonephritis in rheumatoid arthritis

“…There have been discrepancies in the amount of immune complexes and their association with active disease among the various assays (30). However, each assay has been shown to detect similar amounts of immune complexes in patients with extraarticular RA and in SLE patients with glomerulonephritis.…”