Circulating endometrial cells in peripheral blood

Bobek, Vladimír; Kološtová, Katarína; Kucera, E

doi:10.1016/j.ejogrb.2014.07.037

Cited by 19 publications

(17 citation statements)

References 21 publications

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“…While tumor cells need strategies such as EMT in order to escape tight epithelial structures to metastasize, the detachment of endometrial cells is facilitated by the physiological breakdown during menstruation. Indeed, endometrial single cells, clumps of cells, cells with stem cell-like features, and gland-like structures of epithelial and stromal/mesenchymal phenotype, were observed in peripheral blood and menstrual effluent [ 101 , 102 , 103 ].…”

Section: Discussionmentioning

confidence: 99%

Epithelial–Mesenchymal Transition in Endometriosis—When Does It Happen?

Konrad

Dietze

Riaz

et al. 2020

JCM

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Epithelial–mesenchymal transition (EMT) is an important process of cell remodeling characterized by the gradual loss of the epithelial phenotype and progressive gain of a mesenchymal phenotype. EMT is not an all-or-nothing process, but instead a transition of epithelial to mesenchymal cells with intermediate cell states. Recently, EMT was described in endometriosis, and many EMT-specific pathways like Twist, Snail, Slug, Zinc finger E-box-binding homeobox 1/2 (ZEB1/2), E/N-cadherin, keratins, and claudins are involved. However, as pointed out in this review, a comparison of the eutopic endometrium of women with and without endometriosis yielded only subtle changes of these EMT markers. Furthermore, only very few alterations in cell–cell contacts could be found but without changes in the epithelial phenotype. This suggests only a partial EMT which is not a prerequisite for the detachment of endometrial cells and, thus, not critical for the first step(s) in the pathogenesis of endometriosis. In contrast, the majority of changes in the EMT-related marker expression were found in the ectopic endometrium, especially in the three endometriotic entities, ovarian, peritoneal, and deep infiltrating endometriosis (DIE), compared with the eutopic endometrium. In this review, we examine the most important EMT pathways described in endometriosis and propose that partial EMT might result from the interaction of endometrial implants with their surrounding microenvironment.

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Section: Discussionmentioning

confidence: 99%

Epithelial–Mesenchymal Transition in Endometriosis—When Does It Happen?

Konrad

Dietze

Riaz

et al. 2020

JCM

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“…[ 26 , 27 ] Although endometriosis is a benign disease, it has many malignant features such as dissemination, implantation, and metastasis. Bobek et al [ 28 ] firstly reported the presence of endometrial cells in peripheral blood of patients with endometriosis, referred as circulating endometrial cells in 2014. In our own research, [ 10 ] peripheral blood was collected from patients for CEC analysis 1 day before surgery and then the size-based microfluidic chip and immunofluorescence staining were applied to capture and identify CECs.…”

Section: Classical Blood Biomarkersmentioning

confidence: 99%

Current biomarkers for the detection of endometriosis

Tian

Chang

Zhao

et al. 2020

Chinese Medical Journal

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A clinically reliable non-invasive test for endometriosis is expected to reduce the diagnostic delay. Although varieties of biomarkers have been investigated for decades, and cancer antigen-125, cancer antigen-199, interleukin-6, and urocortin were the most studied ones among hundreds of biomarkers, no clinically reliable biomarkers have been confirmed so far. Some emerging technologies including “omics” technologies, molecular imaging techniques, and microRNAs are promising in solving these challenges, but their utility to detect endometriosis has yet to be verified. New combinations of researched indicators or other non-invasive methods and further exploration of the emerging technologies may be new targets and future research hotspots for non-invasive diagnosis of endometriosis. In conclusion, researches of biomarkers for the detection of endometriosis are still ongoing and may benefit from novel molecular biology, bioinformatics methods and a combination of more diverse monitoring methods. Though it will be a daunting task, the identification of a specific set of diagnostic biomarkers will undoubtedly improve the status of endometriosis.

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“…with endometriosis were enrolled in the present study. The direct visualization of the lesions by invasive procedure, using a laparoscopy is currently the primary method of diagnosis (15), and the final diagnosis was verified by histopathology. For endometrial (eutopic) stromal cells, 9 premenopausal patients (n=9; aged 29-43 years, mean 35.06±5.74) who had undergone hysteroscopy for infertility were enrolled; endometrial tissue verified histologically however there was no evidence of endometriosis.…”

Section: Inhibitory Effect Of Curcumin In Human Endometriosis Endometmentioning

confidence: 99%

Inhibitory effect of curcumin in human endometriosis endometrial cells via downregulation of vascular endothelial growth factor

Cao

Wei

Zhou

et al. 2017

Molecular Medicine Reports

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Endometriosis, which affects up to 10% of women of reproductive age, is defined as endometrial-like gland and stroma tissue growths outside the uterine cavity. Despite increasing research efforts, there are no current effective treatment methods for this disease, therefore investigations for therapeutic strategies are of primary concern. In preliminary work, the authors demonstrated that curcumin inhibits endometriosis in vivo. The present in vitro study aimed to investigate the association between endometriotic stromal cells and curcumin and to clarify the underlying mechanism of action. A total of 14 patients with endometriosis were enrolled in the present study. The purity of endometrial stromal cell cultures was proven by standard immunofluorescent staining of vimentin. The cell proliferation and curcumin effects on endometrial stromal cells were assessed by the MTT assay and Hematoxylin and Eosin staining. For cell cycle analysis, phase distribution was detected by flow cytometry. Vascular endothelial growth factor (VEGF) protein expression was examined using immunohistochemistry staining. Apoptosis was assessed using Annexin V‑fluorescein isothiocyanate staining. The results indicated that the treatment of curcumin decreased human ectopic and eutopic stromal cell growth. Following treatment with curcumin, human endometriotic stromal cells demonstrated an increased percentage of G1‑phase cells and decreased percentages of S‑phase cells, particularly in the group treated with 50 µmol/l curcumin. Treatment with curcumin additionally decreased expression of VEGF. The data provide evidence that curcumin reduces cell survival in human endometriotic stromal cells, and this may be mediated via downregulation of the VEGF signaling pathway.

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Circulating endometrial cells in peripheral blood

Cited by 19 publications

References 21 publications

Epithelial–Mesenchymal Transition in Endometriosis—When Does It Happen?

Epithelial–Mesenchymal Transition in Endometriosis—When Does It Happen?

Current biomarkers for the detection of endometriosis

Inhibitory effect of curcumin in human endometriosis endometrial cells via downregulation of vascular endothelial growth factor

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