Circulating cell‐free <scp>DNA</scp> levels in hemodialysis patients and its association with inflammation, iron metabolism, and <scp>rhEPO</scp> doses

Kohlová, Michaela; Ribeiro, Sandra; Faria, Maria do Sameiro; Rocha‐Pereira, Petronila; Fernandes, João; Reis, Flávio; Miranda, Vasco; Quintanilha, Alexandre; Belo, Luı́s; Bronze-Rocha, Elsa; Costa, Elı́sio; Santos-Silva, Alice

doi:10.1111/hdi.12055

Cited by 11 publications

(14 citation statements)

References 10 publications

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“…In contrast, measurement of DNA concentration by our assay requires only the mixture of a small serum or plasma sample with a fluorescent dye; collected blood can be kept at room temperature for few hours, no extraction or incubation are needed and results are promptly available [15]. Our assay was used in previous clinical studies by us [5], [8], [15], [18], [26] and others [27], [28].…”

Section: Discussionmentioning

confidence: 99%

Admission Cell Free DNA Levels Predict 28-Day Mortality in Patients with Severe Sepsis in Intensive Care

et al. 2014

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AimThe aim of the current study is to assess the mortality prediction accuracy of circulating cell-free DNA (CFD) level at admission measured by a new simplified method.Materials and MethodsCFD levels were measured by a direct fluorescence assay in severe sepsis patients on intensive care unit (ICU) admission. In-hospital and/or twenty eight day all-cause mortality was the primary outcome.ResultsOut of 108 patients with median APACHE II of 20, 32.4% have died in hospital/or at 28-day. CFD levels were higher in decedents: median 3469.0 vs. 1659 ng/ml, p<0.001. In multivariable model APACHE II score and CFD (quartiles) were significantly associated with the mortality: odds ratio of 1.05, p = 0.049 and 2.57, p<0.001 per quartile respectively. C-statistics for the models was 0.79 for CFD and 0.68 for APACHE II. Integrated discrimination improvement (IDI) analyses showed that CFD and CFD+APACHE II score models had better discriminatory ability than APACHE II score alone.ConclusionsCFD level assessed by a new, simple fluorometric-assay is an accurate predictor of acute mortality among ICU patients with severe sepsis. Comparison of CFD to APACHE II score and Procalcitonin (PCT), suggests that CFD has the potential to improve clinical decision making.

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Section: Discussionmentioning

confidence: 99%

Admission Cell Free DNA Levels Predict 28-Day Mortality in Patients with Severe Sepsis in Intensive Care

et al. 2014

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“…Based on fairly recent findings that release of cell free DNA takes place during hemodialysis (Tovbin et al, 2012) due to cell death, Kohlova et al (2013) investigated the levels of cell-free DNA in end-stage renal disease patients (58% men, mean age 65.7±14.4 years, n=153 and 20 controls) and its association with other hematological, inflammatory and iron status indicators. The patients had significantly lower serum iron and transferrin and significantly higher sTfR, transferrin saturation, ferritin, hepcidin CRP, IL-6 and circulating free DNA (p<0.05 in all instances).…”

Section: Assessment Of Iron Status In Kidney Failure and Health Outcomementioning

confidence: 99%

Iron status in the elderly: A review of recent evidence

Wawer

Jennings

Fairweather‐Tait

2018

Mechanisms of Ageing and Development

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A comprehensive literature review of iron status in the elderly was undertaken in order to update a previous review (Fairweather-Tait et al, 2014); 138 summarised papers describe research on the magnitude of the problem, aetiology and age-related physiological changes that may affect iron status, novel strategies for assessing iron status with concurrent health conditions, hepcidin, lifestyle factors, iron supplements, iron status and health outcomes (bone mineral density, frailty, inflammatory bowel disease, kidney failure, cancer, cardiovascular, and neurodegenerative diseases). Each section of this review concludes with key points from the relevant papers. The overall findings were that disturbed iron metabolism plays a major role in a large number of conditions associated with old age. Correction of iron deficiency/overload may improve disease prognosis, but diagnosis of iron deficiency requires appropriate cut-offs for biomarkers of iron status in elderly men and women to be agreed. Iron deficiency (with or without anemia), anemia of inflammation, and anemia of chronic disease are all widespread in the elderly and, once identified, should be investigated further as they are often indicative of underlying disease. Management options should be reviewed and updated, and novel therapies, which show potential for treating anemia of inflammation or chronic disease, should be considered.

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“…[5][6][7] In patients on haemodialysis, CFD levels were associated with inflammation 8 and positively correlated with interleukin (IL)-6. 7,8 We found that IL-6 levels are increased in psoriasis and correlate with the Psoriasis Area and Severity Index (PASI). 9 Considering the inflammatory and hyperproliferative psoriasis features and that, as far as we know, CFD levels have not been evaluated in psoriasis, we thought it important to evaluate them.…”

Section: What Does This Study Add?mentioning

confidence: 97%

Circulating cell-free DNA levels in Portuguese patients with psoriasis vulgaris according to severity and therapy

et al. 2014

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SummaryBackground Inflammation has a key role in the pathogenesis of psoriasis. Circulating cell-free DNA (CFD) is a marker of tissue cell damage closely associated with inflammation. Objectives We aimed to understand the relation of CFD levels with psoriasis severity, defined by the Psoriasis Area and Severity Index (PASI), with inflammation and with psoriasis therapy. Methods Forty-six patients with psoriasis vulgaris were evaluated before (T0) and after 12 weeks (T12) of treatment with narrowband ultraviolet light B (NB-UVB; n = 17), psoralen plus UVA (PUVA; n = 20) or topical therapy (n = 9). We evaluated interleukin (IL)-6 and circulating CFD levels. Results Compared with controls, at T0, patients presented significantly higher levels of circulating CFD. CFD presented a significant positive correlation with IL-6 and a trend towards a positive correlation with PASI. Multiple linear regression analysis identified IL-6 as an independent variable associated with CFD circulating levels. As shown by the PASI score, a trend towards higher values of CFD was observed in the severe psoriasis forms; moderate and severe psoriasis presented also significantly higher CFD values, compared with control. Both NB-UVB and PUVA treatments significantly decreased the levels of CFD. Conclusions Patients with psoriasis, at the active stage of the disease, presented an increased inflammation associated with raised circulating CFD levels, which seem to be linked to psoriasis severity. Both NB-UVB and PUVA, anti-inflammatory therapies, were effective in decreasing CFD values. We propose that the evaluation of circulating CFD may provide a new biomarker to monitor psoriasis, its severity and its treatment.

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Circulating cell‐free DNA levels in hemodialysis patients and its association with inflammation, iron metabolism, and rhEPO doses

Cited by 11 publications

References 10 publications

Admission Cell Free DNA Levels Predict 28-Day Mortality in Patients with Severe Sepsis in Intensive Care

Admission Cell Free DNA Levels Predict 28-Day Mortality in Patients with Severe Sepsis in Intensive Care

Iron status in the elderly: A review of recent evidence

Circulating cell-free DNA levels in Portuguese patients with psoriasis vulgaris according to severity and therapy

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