“…1 H NMR (500 MHz, DMSO-d 6 ): δ 7.91 2H),3H),6.71 (s,1H),3H),2H),1.86 (dddd,J = 13.4,9.3,6.6,5.4 Hz,1H),1H) 9, 170.8, 156.2, 144.2, 141.1, 138.4, 128.11, 127.6, 127.5, 125.8, 125.7, 122.0, 121.6, 121.4, 120.6, 119.6, 66.4, 60.2, 53.4, 47.0, 31.7, 28. Peptides, and Protein Expression and Purification. 35,36 Wild-type human β-catenin (residues 138-781) were cloned into a pET-28b vector carrying a C-terminal 6× histidine (Novagen) and transformed into Escherichia coli BL21 DE3 (Novagen). Cells were cultured in LB medium with 30 μg/mL kanamycin until the OD 600 was approximately 0.8.…”