Circulating Biomarkers and their Possible Role in Pathogenesis of Chronic Hepatitis B and C Viral Infections

Khan, Saba; Bhargava, Arpit; Pathak, Neelam; Maudar, K K; Varshney, Subodh; Mishra, Pradyumna Kumar

doi:10.1007/s12291-010-0098-7

Cited by 23 publications

(27 citation statements)

References 38 publications

(36 reference statements)

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“…Precisely, roles and relationship between the 2 pathways in the tumor development depend on their specific interactions between genetic and epigenetic factors and environmental factors. Acute or chronic exposures to chemicals and infectious agents are known to create an inflammatory microenvironment that facilitates initiation of carcinogenesis [22,23]. In this context, the pro-inflammatory cytokine response in human lymphocytes and neutrophils; human lung fibroblasts (IMR-90), human colon epithelial (FHC) cells treated with 0.005 μM (1 μg/μl) MIC were evaluated.…”

Section: Chronic Inflammationmentioning

confidence: 99%

“…A persistent hyper-responsive cellular and humoral immune state observed 2 decades later in individuals in utero exposed to MIC during the Bhopal gas tragedy offers a potential insight into epigenetic modulation of the immune system [70]. A retrospective surveillance for monitoring possible health effects for the hepatitis infections amongst the 1st and 2nd generations of survivors of Bhopal gas tragedy revealed hepatitis virus infections to be most common among the MIC exposed cohort [71][72][73][74]. Genotype 3 of hepatitis C virus (HCV) was the most prevalent HCV genotype among the survivors [75,76].…”

Section: Infection-driven Cancers and Epigenomic Linksmentioning

confidence: 99%

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Molecular bio-dosimetry for carcinogenic risk assessment in survivors of Bhopal gas tragedy

Mishra¹,

Raghuram²,

Bunkar³

et al. 2015

Int J Occup Med Environ Health

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International Journal of Occupational Medicine and Environmental AbstractDecember 2014 marked the 30th year anniversary of Bhopal gas tragedy. This sudden and accidental leakage of deadly poisonous methyl isocyanate (MIC) gas instigated research efforts to understand the nature, severity of health damage and sufferings of 570 000 ailing survivors of this tragedy. In a decade-long period, our systematic laboratory investigations coupled with long-term molecular surveillance studies have comprehensively demonstrated that the risk of developing an environmental associated aberrant disease phenotype, including cancer, involves complex interplay of genomic and epigenetic reprogramming. These findings poised us to translate this knowledge into an investigative framework of "molecu lar biodosimetry" in a strictly selected cohort of MIC exposed individuals. A pragmatic cancer risk-assessment strategy pursued in concert with a large-scale epidemiological study might unfold molecular underpinnings of host-susceptibility and exposureresponse relationship. The challenges are enormous, but we postulate that the study will be necessary to establish a direct initiation-promotion paradigm of environmental carcinogenesis. Given that mitochondrial retrograde signaling-induced epigenetic reprogramming is apparently linked to neoplasticity, a cutting-edge tailored approach by an expert pool of biomedical researchers will be fundamental to drive these strategies from planning to execution. Validating the epigenomic signatures will hopefully result in the development of biomarkers to better protect human lives in an overburdened ecosystem, such as India, which is continuously challenged to meet population demands. Besides, delineating the mechanistic links between MIC exposure and cancer morbidity, our investigative strategy might help to formulate suitable regulatory policies and measures to reduce the overall burden of occupational and environmental carcinogenesis.

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Section: Chronic Inflammationmentioning

confidence: 99%

Section: Infection-driven Cancers and Epigenomic Linksmentioning

confidence: 99%

Molecular bio-dosimetry for carcinogenic risk assessment in survivors of Bhopal gas tragedy

Mishra¹,

Raghuram²,

Bunkar³

et al. 2015

Int J Occup Med Environ Health

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“…is monitored by the decrease in absorbance at 340 nm, which is directly proportional to the GR activity in the sample [14]. SOD in the cells was also evaluated through ELISA, as reported previously [15]. The percentage inhibition of the formation of nitro blue tetrazolium (NBT)-diformazan by SOD was converted to the relative activity of the sample, expressed as mU/mL at the absorbance of 450 nm.…”

Section: Measurement Of Anti-oxidant Defense Enzymesmentioning

confidence: 99%

Impairment of Mitochondrial–Nuclear Cross Talk in Neutrophils of Patients with Type 2 Diabetes Mellitus

Khan

Raghuram²,

Pathak

et al. 2013

Ind J Clin Biochem

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Increased leukocyte apoptosis is intrinsically linked to disease patho-physiology, susceptibility to and severity of infections in type 2 diabetes mellitus (T2DM) patients. A consistent defect in neutrophil function is considered central to this increased risk for infections. Although redox imbalance is considered a potential mediator of these associated complications, detailed molecular evidence in clinical samples remains largely undetected. The study consisted of three groups (n = 50 each) of Asian Indians: early diagnosed diabetic patients, cases with late-onset diabetic complications and age and gender-matched healthy controls. We evaluated mitochondrial oxidative stress, levels of nuclear DNA damage and apoptosis in peripheral blood neutrophils isolated from T2DM patients. We observed that in both early and late diabetic subjects, the HbA1c levels in neutrophils were altered considerably with respect to healthy controls. Increased oxidative stress observed in both early and late diabetics imply the disentanglement of fine equilibrium of mitochondria-nuclear cross talk which eventually effected the augmentation of downstream nuclear cH2AX activation and caspase-3 expression. It would be overly naïve to refute the fact that mitochondrial deregulation in neutrophils perturbs immunological balance in type 2 diabetic conditions. By virtue of our data, we posit that maneuvering mitochondrial function might offer a prospective and viable method to modulate neutrophil function in T2DM. Nevertheless, similar investigations from other ethnic groups in conjunction with experimental evidences would be a preeminent need. Obviously, our study might aid to comprehend this complex interplay between mitochondrial dysfunction and neutrophil homeostasis in T2DM.

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“…The amplicons were generated and quantified in COBAS as per standard protocol following all the necessary instructions from the supplier. 21 The obtained values have a sensitivity of 1,620 copies/mL (600 IU/mL as per manual).…”

Section: Rna Extraction and Quantification From Serummentioning

confidence: 99%

Novel Approach for Quantification of Hepatitis C Virus in Liver Cirrhosis Using Real-Time Reverse Transcriptase PCR

Maudar

Gandhi

Mishra

et al. 2012

Journal of Gastrointestinal Surgery

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Circulating Biomarkers and their Possible Role in Pathogenesis of Chronic Hepatitis B and C Viral Infections

Cited by 23 publications

References 38 publications

Molecular bio-dosimetry for carcinogenic risk assessment in survivors of Bhopal gas tragedy

Molecular bio-dosimetry for carcinogenic risk assessment in survivors of Bhopal gas tragedy

Impairment of Mitochondrial–Nuclear Cross Talk in Neutrophils of Patients with Type 2 Diabetes Mellitus

Novel Approach for Quantification of Hepatitis C Virus in Liver Cirrhosis Using Real-Time Reverse Transcriptase PCR

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