Circulating Anodic Antigen for Detection of Schistosoma mansoni Infection in Egyptian Patients

Elmorshedy, Hala; Kinosien, Bruce; Barakat, Rashida; Omer, E.; Khamis, Nadia; Deelder, André M.; Phillips, Michael

doi:10.4269/ajtmh.1996.54.149

Cited by 21 publications

(13 citation statements)

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“…At 12 weeks following praziquantel treatment, 87% of the serum samples (80/92) and 81.5% of the urine specimens (75/ 92) from S. haematobium-infected patients turned negative. A reduction in CSA levels following speci®c chemotherapy has also been reported by other investigators (De Jonge et al 1988, 1989aShaker et al 1992;Van Leishout et al 1991El-Morshedy et al 1996;Hassan et al 1998). The pro®le of CSA in urine following praziquantel treatment shows a parallel, albeit delayed, decline relative to that detected in serum.…”

Section: Csa Level After Chemotherapysupporting

confidence: 57%

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

Salah

Demerdash

Shaker

et al. 2000

Parasitology Research

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A monoclonal antibody (mAb), 2F/11F, raised against Schistosoma haematobium soluble egg antigen (SEA) was found to be nonreactive with S. mansoni SEA or other parasite antigens (Fasciola hepatica, Echinococcus granulosus). This IgG1 mAb recognized a repetitive epitope on S. haematobium SEA in the molecular-weight regions of 70, 42, and 35 kDa. It was employed as both an antigen-capture and a biotinylated detection antibody in a sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of circulating schistosome antigen (CSA) and had a detection limit of <1 ng S. haematobium SEA/ml. CSA levels were measured in serum and urine samples from 116 S. haematobium-infected rural students before therapy and at 4, 8, and 12 weeks after praziquantel treatment. Serum and urine samples from 50 S. mansoni -infected patients, 15 patients harboring other parasites, and 30 noninfected individuals were also assessed. CSA was detected in 90.5% of serum samples and 94% of urine samples from S. haematobium-infected patients. CSA was undetectable in serum from the 15 patients harboring other parasites and in 94% of serum samples and 84% of urine samples from S. mansoni-infected patients. In the S. haematobium-infected group a positive correlation was detected between CSA levels in serum and urine samples and the egg load per 10 ml urine. A significant reduction in CSA levels was detected in serum and urine samples after praziquantel therapy. CSA was undetectable in 87% of serum samples and 81.5% of urine samples from schistosomiasis haematobium patients at 12 weeks post-treatment. These data demonstrate that the use of mAb 2F/11F for detection of CSA provides a sensitive method for the immunodiagnosis and monitoring of cure of schistosomiasis haematobium.

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Section: Csa Level After Chemotherapysupporting

confidence: 57%

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

Salah

Demerdash

Shaker

et al. 2000

Parasitology Research

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“…The diagnosis was based on immunofluorescent demonstration of antibodies against schistosome excretory/secretory antigens present in the gut of adult worms (anti-GAA) (Deelder & Kornelis 1981, Mott & Dixon 1982, Feldmeier & Poggensee 1993, whereas no antibodies could be seen in assays for antibodies against schistosome egg antigens (Pitkänen et al 1990). Alternative parasite-specific serodiagnostic methods for the detection of early schistosomiasis are either of low sensitivity (detection of circulating schistosome antigens) (Van Dam et al 1993, Van't Wout et al 1995, El-Morshedy et al 1996, Ndhlovu et al 1996, Van Etten et al 1996 or not easily available (an adult worm antigen fraction) (Hancock & Tsang 1986, Tsang & Wilkins 1997.…”

Section: Introductionmentioning

confidence: 99%

Cross reacting antibodies against keyhole limpet haemocyanin may interfere with the diagnostics of acute schistosomiasis

Thors¹,

Linder²

1998

Parasite Immunology

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The number of individuals catching schistosomiasis has increased with the popularity of 'primitive tourism' in Africa. Highly immunogenic material originating from the intestine of intravascular adult schistosomes gives rise to an antibody response making possible early identification of infected individuals using serology. Antibodies against gut associated antigens (anti-GAA), detected by indirect immunofluorescence microscopy employing sections of adult worms as antigen may occur before the onset of egg production. In the present study we show that this well known schistosomiasis-specific anti-GAA staining reaction can be confused with a similar staining reaction with ducts of both male and female worms. Antibodies with duct reactivity were seen in sera both from schistosomiasis-patients and patients with some other invasive worm infections. Cross reactive anti-duct antibodies appear to have different specificity. One cross reactive antibody reacted with antigenic epitopes present in keyhole limpet haemocyanin (KLH). Anti-duct reactivity could be inhibited by absorption with KLH. This was most obvious in the trichinellosis patient sera.

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“…Under certain conditions, some serologic tests can better estimate the real prevalence of this parasitosis than the classical stool exams (Noya et al 1999). Techniques for detection of circulating antigens have not attained yet an acceptable degree of sensitivity for the diagnosis of individuals with low parasitic loads (El-Morshedy et al 1996), but methodologies that detect specific antibodies have shown sensitivity indices close to 100% (Lima et al 1996). The indirect immunofluorescence tests on worm paraffin (IgM-IFT) or frozen sections (IgG-IFT) have presented satisfactory degrees of sensitivity and specificity for diagnosis of schistosomiasis (Kanamura et al 1979, Deelder & Kornelis 1981, Silva et al 1992.…”

Section: Discussionmentioning

confidence: 99%

Schistosomiasis mansoni: follow-up of control program based on parasitologic and serologic methods in a Brazilian community of low endemicity

Burlandy-Soares

Dias

Kanamura

et al. 2003

Mem. Inst. Oswaldo Cruz

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Circulating Anodic Antigen for Detection of Schistosoma mansoni Infection in Egyptian Patients

Cited by 21 publications

References 0 publications

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

Cross reacting antibodies against keyhole limpet haemocyanin may interfere with the diagnostics of acute schistosomiasis

Schistosomiasis mansoni: follow-up of control program based on parasitologic and serologic methods in a Brazilian community of low endemicity

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