Circular RNAs in Vascular Functions and Diseases

Ding, Shengguang; Zhu, Yulong; Liang, Yajun; Huang, Haitao; Xu, Yiming; Zhong, Chongjun

doi:10.1007/978-981-13-1426-1_23

Cited by 43 publications

(14 citation statements)

References 115 publications

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“…These characteristics provide circRNA better prospect for biomarker of human disease. Importantly, a growing amount of evidences have illustrated the association between circRNAs expression and atherosclerosis progress [21, 33], but little known is about the role of circRNA in endothelial phenotype involved in atherosclerosis. In this study, we verified increased expression of circ_0003204 in ox-LDL treated HAECs as well as EVs from HAEC culture media and plasma of the patients with cerebral atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Zhang¹,

Song²,

Yuan³

et al. 2020

J Biomed Sci

108

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Background: Circular RNAs (circRNAs) represent a class of non-coding RNAs (ncRNAs) which are widely expressed in mammals and tissue-specific, of which some could act as critical regulators in the atherogenesis of cerebrovascular disease. However, the underlying mechanisms by which circRNA regulates the ectopic phenotype of endothelial cells (ECs) in atherosclerosis remain largely elusive. Methods: CCK-8, transwell, wound healing and Matrigel assays were used to assess cell viability, migration and tube formation. QRT-qPCR and Immunoblotting were used to examine targeted gene expression in different groups. The binding sites of miR-370-3p (miR-370) with TGFβR2 or hsa_circ_0003204 (circ_0003204) were predicted using a series of bioinformatic tools, and validated using dual luciferase assay and RNA immunoprecipitation (RIP) assay. The localization of circ_0003204 and miR-370 in ECs were investigated by fluorescence in situ hybridization (FISH). Gene function and pathways were enriched through Metascape and gene set enrichment analysis (GSEA). The association of circ_0003204 and miR-370 in extracellular vesicles (EVs) with clinical characteristics of patients were investigated using multiple statistical analysis. Results: Circ_0003204, mainly located in the cytoplasm of human aorta endothelial cells (HAECs), was upregulated in the ox-LDL-induced HAECs. Functionally, the ectopic expression of circ_0003204 inhibited proliferation, migration and tube formation of HAECs exposed to ox-LDL. Mechanically, circ_0003204 could promote protein expression of TGFβR2 and its downstream phosph-SMAD3 through sponging miR-370, and miR-370 targeted the 3′ untranslated region (UTR) of TGFβR2. Furthermore, the expression of circ_0003204 in plasma EVs was upregulated in the patients with cerebral atherosclerosis, and represented a potential biomarker for diangnosis and prognosis of cerebrovascular atherogenesis. Conclusions: Circ_0003204 could act as a novel stimulator for ectopic endothelial inactivation in atherosclerosis and a potential biomarker for cerebral atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Zhang¹,

Song²,

Yuan³

et al. 2020

J Biomed Sci

108

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“…6 Circular RNAs play a pivotal role in cardiovascular diseases. 7 The first evidence suggesting the role of circRNA in regulating heart physiology and pathology came from studies of heart-related cirRNAs (CRHR) which act as an endogenous sponge of miR-223, thereby inhibiting the miR-223-induced cardiac hypertrophy and heart failure in mice. 8,9 Microarray analyses of dysregulated circRNAs indicate detection of 1,163 circRNAs, out of which 63 circRNAs are differentially expressed in heart tissue of mice with myocardial infarction-induced heart failure.…”

Section: Introductionmentioning

confidence: 99%

Silencing of circHIPK3 Inhibits Pressure Overload-Induced Cardiac Hypertrophy and Dysfunction by Sponging miR-185-3p

Xu¹,

Wang²,

Wang³

2020

DDDT

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“…Recently, abnormal circRNA expressions have been demonstrated in various cardiovascular diseases and cancers, which are related to vascular dysfunction. [6][7][8] Knockdown of cZNF609, which is highly enriched in endothelial cells, diminished retinal vessel loss and inhibited pathological angiogenesis in vivo. 9 CircRNA USP1 (circ-USP1, also known as hsa_circ_0000080 according to circBase), which is located at chr1p31, is derived from the back-splicing of exon 6-8 of ubiquitin-specific peptidase 1 (USP1) gene.…”

mentioning

confidence: 99%

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao

et al. 2019

J Cellular Molecular Medi

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Recent studies indicate circular RNAs are related to dysregulation of vascular endothelial cell function, yet the underlying mechanisms have remained elusive. Here, we characterized the functional role of circular RNA USP1 (circ‐USP1) in the regulation of the blood‐tumour barrier (BTB) permeability and the potential mechanisms. In the current study, the circ‐USP1 expressing level was up‐regulated in glioma cerebral microvascular endothelial cells (GECs) of the BTB model in vitro. Knockdown of circ‐USP1 disrupted the barrier integrity, increased its permeability as well as reduced tight junction‐related protein claudin‐5, occludin and ZO‐1 expressions in GECs. Bioinformatic prediction and luciferase assay indicated that circ‐USP1 bound to miR‐194‐5p and suppressed its activity. MiR‐194‐5p contributed to circ‐USP1 knockdown‐induced increase of BTB permeability via targeting and down‐regulating transcription factor FLI1. Furthermore, FLI1 regulated the expressions of claudin‐5, occludin and ZO‐1 in GECs through binding to their promoter regions. Single or combined treatment of circ‐USP1 and miR‐194‐5p effectively promoted anti‐tumour drug doxorubicin across BTB to induce apoptosis of glioma cells. Overall, this present study identified the crucial regulation of circ‐USP1 on BTB permeability via miR‐194‐5p/FLI1 axis‐mediated regulation of tight junction proteins, which might facilitate the development of therapeutics against human gliomas.

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Circular RNAs in Vascular Functions and Diseases

Cited by 43 publications

References 115 publications

RETRACTED ARTICLE: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

RETRACTED ARTICLE: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Silencing of circHIPK3 Inhibits Pressure Overload-Induced Cardiac Hypertrophy and Dysfunction by Sponging miR-185-3p

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

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