Circular RNA microarray expression profile in 3,4‐benzopyrene/angiotensin II‐induced abdominal aortic aneurysm in mice

Wang, Jiaoni; Sun, Huankun; Zhou, Yingying; Huang, Kaiyu; Que, Jia-Qun; Peng, Yu; Wang, Jinsheng; Lin, Cong; Xue, Yan; Ji, Kangting

doi:10.1002/jcb.28333

Cited by 18 publications

(16 citation statements)

References 37 publications

(61 reference statements)

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“…24 In AAA, circRNAs expression profiling indicated the potential participation of circRNAs in AAA. 18 Consistent with previous work, we first demonstrated that circRNACCDC66 was elevated in Angiotensin II-stimulated VSMCs, which is prerequisite for probing its function in AAA. As documented previously, circRNACCDC66 was tumour-facilitating in gastric cancer, 25 colon cancer, 26 and lung adenocarcinoma.…”

Section: Discussionsupporting

confidence: 90%

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Circular RNA CCDC66 facilitates abdominal aortic aneurysm through the overexpression of CCDC66

Yang

Wang

Meng

et al. 2020

Cell Biochemistry & Function

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Funding information The fifth group of bosom friends and talents plan of Hanyang District, Wuhan Abdominal aortic aneurysm (AAA) is fatal meanwhile unpredictable asymptomatic cardiovascular disease. Available data suggests the potential participation of circular RNAs (circRNAs) in AAA pathogenesis. But direct evidence is limited. The present study is to functionally and mechanically characterize circRNA CCDC66 (circCCDC66) in AAA. Previous work indicated the differentially expressed circCCDC66 in AAA. At molecular level, circCCDC66, miR-342-3p and CCDC66 transcript were measured through real-time quantitative polymerase chain reaction assay. Functionally, we examined the cellular behaviours of circCCDC66-depleted or CCDC66-depleted vascular smooth muscle cells (VSMCs) including proliferation and apoptosis. It elucidated that depletion of circCCDC66 induced proliferation facilitation and apoptosis reduction. Mechanically, we addressed the interplay among circCCDC66, miR-342-3p and CCDC66 transcript using RNA immunoprecipitation, RNA pull-down and luciferase reporter experiments. Through mechanical validation, we discovered the positive regulation of circCCDC66 on its host gene CCDC66. Loss of CCDC66 mimicked the effects of circCCDC66 silencing on VSMC growth. Moreover, it uncovered that circCCDC66 regulated CCDC66-dependent VSMC growth through sponging miR-342-3p. Rescue experiments aimed to address the functional role of regulatory network formed by circCCDC66, miR-342-3p and CCDC66 in VSMC growth and apoptosis. Suppressing miR-342-3p or overexpressing CCDC66 could reverse VSMC growth caused by circCCDC66 deficiency. Our study further emphasized and first unveiled the function of circCCDC66 in VSMC proliferation. CircCCDC66 upregulated its host gene through its role of miR-342-3p sponge, and hinted a novel molecular mechanism in AAA. Significance of the study: It was firstly displayed in our study that depletion of circCCDC66 induced proliferation augmentation and apoptosis reduction of vascular smooth muscle cells (VSMCs). Meanwhile, circCCDC66/miR-342-3p/CCDC66 axis was proved can play the function of modulating the cell proliferation and apoptosis of VSMCs, which provided us a novel molecular mechanism in AAA.

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Section: Discussionsupporting

confidence: 90%

“…Circular RNA CCDC66 (circCCDC66) was an upregulated circRNA in AAA from previous revelation. 18 Consequently, we probed the function of circCCDC66 in VSMC apoptosis and proliferation via multiple assays in this study. Besides, we explored whether circCCDC66 could modulate its host gene to function in AAA.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA CCDC66 facilitates abdominal aortic aneurysm through the overexpression of CCDC66

Yang

Wang

Meng

et al. 2020

Cell Biochemistry & Function

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“…Overexpression of circ_104916 inhibits migration and invasion of colorectal cancer cells via repressing epithelial to mesenchymal transition (EMT) [36]. Wang J et al discovered six circRNAs related to apoptosis, which induced abdominal aortic aneurysm via promoting endothelial cells growth from circRNA-miRNA networks [37]. Circ_0005015 facilitates retinal endothelial angiogenesis by regulating proliferation, migration and tube formation of endothelial cells [38].…”

Section: Discussionmentioning

confidence: 99%

SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway

Liu

Shen

Zhu

et al. 2020

J Exp Clin Cancer Res

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Background: Angiogenesis plays an important role in the progress of glioma. RNA-binding proteins (RBPs) and circular RNAs (circRNAs), dysregulated in various tumors, have been verified to mediate diverse biological behaviors including angiogenesis. Methods: Quantitative real-time PCR (qRT-PCR) and western blot were performed to detect the expression of SRSF10, circ-ATXN1, miR-526b-3p, and MMP2/VEGFA. The potential function of SRSF10/circ-ATXN1/miR-526b-3p axis in glioma-associated endothelial cells (GECs) angiogenesis was further studied. Results: SRSF10 and circ-ATXN1 were significantly upregulated in GECs compared with astrocyte-associated endothelial cells (AECs). Knockdown of SRSF10 or circ-ATXN1 significantly inhibited cell viability, migration and tube formation of GECs where knockdown of SRSF10 exerted its function by inhibiting the formation of circ-ATXN1. Moreover, the combined knockdown of SRSF10 and circ-ATXN1 significantly enhanced the inhibitory effects on cell viability, migration and tube formation of GECs, compared with knockdown of SRSF10 and circ-ATXN1, respectively. MiR-526b-3p was downregulated in GECs. Circ-ATXN1 functionally targeted miR-526b-3p in an RNA-induced silencing complex. Up-regulation of miR-526b-3p inhibited cell viability, migration and tube formation of GECs. Furthermore, miR-526b-3p affected the angiogenesis of GECs via negatively regulating the expression of MMP2/ VEGFA. Conclusion: SRSF10/circ-ATXN1/miR-526b-3p axis played a crucial role in regulating the angiogenesis of GECs. The above findings provided new targets for anti-angiogenic therapy in glioma.

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“…Previously, Zheng et al found that hsa_circ_000595 was upregulated in human AAA tissues, which would reduce the expression of miR-19a and subsequently promote human vascular smooth muscle cells (VSMC) apoptosis [31]. Similarly, apoptosis-related circRNAs were observed altered in AAA animal models [26]. Besides, circWDR77/ miR-124/FGF-2 and circSATB2/miR-939/STIM1 regulatory axis are verified to regulate VSMC proliferation and migration [32,33].…”

Section: Discussionmentioning

confidence: 97%

“…Using gene expression profile, several researchers detected that immune and inflammatory response had a great impact on the pathogenesis of AAA [22][23][24]. Recently, advances in novel computational approaches and high-throughput sequencing techniques have sparked new interest in the research on noncoding RNAs [25,26]. As an important member of noncoding RNAs, cir-cRNAs are abundant, stable and highly conserved.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

Zhou

Shi

Cai

et al. 2020

BMC Cardiovasc Disord

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Background: This study aimed to identify the differentially expressed circular RNAs (circRNAs) between human abdominal aortic aneurysm (AAA) and the control group. Methods: High-throughput sequencing was applied to determine the circRNA expression profiles of 4 paired aortic samples. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was carried out to testify 6 randomly selected dysregulated circRNAs. Kyoto Encyclopedia of Genes and Genomes and Gene ontology (GO) analysis were conducted for functional annotation of the parental genes. Additionally, interaction networks between circRNA and 5 putative microRNA (miRNA) partners were constructed.Results: Finally, 411 differentially expressed circRNAs were discovered, including 266 downregulated and 145 upregulated circRNAs. Compared with the control group, the expression level of hsa (Homo sapiens) _circ_0005360 (LDLR) and hsa_circ_0002168 (TMEM189) were proved significantly lower in the AAA group by qRT-PCR. Regarding upregulated circRNAs, the most enriched GO molecular function, biological process and cellular component terms were poly(A) RNA binding, negative regulation of transcription from RNA polymerase II promoter and nucleoplasm, respectively. Moreover, circRNA/miRNA interaction networks showed that hsa_circ_0005360/miR-181b and hsa_circ_ 0002168/miR-15a axis might have a regulative role in human AAA. Conclusions: This study revealed new circRNAs potentially related to the pathogenesis of AAA. Further experimental studies are warranted to clarify the potential molecular mechanisms.

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Circular RNA microarray expression profile in 3,4‐benzopyrene/angiotensin II‐induced abdominal aortic aneurysm in mice

Cited by 18 publications

References 37 publications

Circular RNA CCDC66 facilitates abdominal aortic aneurysm through the overexpression of CCDC66

Circular RNA CCDC66 facilitates abdominal aortic aneurysm through the overexpression of CCDC66

SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway

Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

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