Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

Abstract

Background: This study aimed to identify the differentially expressed circular RNAs (circRNAs) between human abdominal aortic aneurysm (AAA) and the control group. Methods: High-throughput sequencing was applied to determine the circRNA expression profiles of 4 paired aortic samples. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was carried out to testify 6 randomly selected dysregulated circRNAs. Kyoto Encyclopedia of Genes and Genomes and Gene ontology (GO) analysis were conducted for functional annotation of the parental genes. Additionally, interaction networks between circRNA and 5 putative microRNA (miRNA) partners were constructed.Results: Finally, 411 differentially expressed circRNAs were discovered, including 266 downregulated and 145 upregulated circRNAs. Compared with the control group, the expression level of hsa (Homo sapiens) _circ_0005360 (LDLR) and hsa_circ_0002168 (TMEM189) were proved significantly lower in the AAA group by qRT-PCR. Regarding upregulated circRNAs, the most enriched GO molecular function, biological process and cellular component terms were poly(A) RNA binding, negative regulation of transcription from RNA polymerase II promoter and nucleoplasm, respectively. Moreover, circRNA/miRNA interaction networks showed that hsa_circ_0005360/miR-181b and hsa_circ_ 0002168/miR-15a axis might have a regulative role in human AAA. Conclusions: This study revealed new circRNAs potentially related to the pathogenesis of AAA. Further experimental studies are warranted to clarify the potential molecular mechanisms.

Circular RNA in cardiovascular disease: Expression, mechanisms and clinical prospects

“…Moreover, differentially expressed circRNAs were discovered in both mice and human with abdominal aortic aneurysm (AAA) 48,49 . Specially, circCCDC66 was upregulated in AAA 48 .…”

Identification of potential novel biomarkers for abdominal aortic aneurysm based on comprehensive analysis of circRNA‑miRNA‑mRNA networks

“…First, appropriate public datasets of human AAA were collected by using Gene Expression Omnibus (GEO) database in National Center of Biotechnology Information (http://www.ncbi.nlm.nih.gov/geo/). Overall, three microarray datasets (GSE7084, GSE57691 and GSE144431) were included in the present study (Table SI) (17)(18)(19). In GSE7084, seven AAA samples and eight healthy samples were involved for mRNA expression profiles by GPL2507 platform.…”

Circular RNA Expression: Its Potential Regulation and Function in Abdominal Aortic Aneurysms

“…However, the functional role(s) of these circRNAs in AAA development in animal models and their therapeutic potential are yet to be elucidated. [83]. Finally, a total of 411 differentially expressed circRNAs were detected in AAA samples, including 145 upregulated cir-cRNAs and 266 downregulated circRNAs.…”

“…Zhou et al from China observed differential expression of circRNAs in AAA samples and controls, so they subsequently conducted high-throughput sequencing to determine the circRNA expression patterns in four pairs of aortic samples, including four consecutive AAA cases undergoing open surgery and four brain-dead heart-beating organ samples [ 83 ]. Finally, a total of 411 differentially expressed circRNAs were detected in AAA samples, including 145 upregulated circRNAs and 266 downregulated circRNAs.…”