Circular RNA Expression in Ovarian Endometriosis

Shen, Licong; Zhang, Yu; Zhou, Wenjun; Zheng, Peng; Hong, Xiaxia; Zhang, Yi

doi:10.2217/epi-2017-0079

Cited by 24 publications

(16 citation statements)

References 40 publications

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“…The basic characters of the DE circRNAs were largely in accordance with other diseases and cell types in chromosomal distribution, categories and length [ 48 , 49 ]. In our study, we found the number of up-regulated circRNAs (912/1365) was higher than that of down-regulated circRNAs (453/1365), and this result is in accordance with the circRNA profiles in some other diseases and cell types, such as in radioresistant esophageal cancer cells [ 50 ], but is different than the circRNA profiles in 7-month-old senescence accelerated mouse prone 8 (SAMP8) model brains [ 51 ] and other diseases [ 52 ].…”

Section: Discussionsupporting

confidence: 58%

Crosstalk in competing endogenous RNA networks reveals new circular RNAs involved in the pathogenesis of early HIV infection

Zhang

et al. 2018

J Transl Med

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BackgroundThe events in early HIV infection (EHI) are important determinants of disease severity and progression rate to AIDS, but the mechanisms of pathogenesis in EHI have not been fully understood. Circular RNAs (circRNAs) have been verified as “microRNA sponges” that regulate gene expression through competing endogenous RNA (ceRNA) networks, but circRNA expression profiles and their contribution to EHI pathogenesis are still unclear.MethodsTwo different libraries were constructed with RNA from human peripheral blood mononuclear cells from 3 HARRT-naive EHI patients and 3 healthy controls (HCs). The complete transcriptomes were sequenced with RNA sequencing (RNA-Seq) and miRNA sequencing (miRNA-Seq). The differentially expressed (DE) RNAs were validated with RT-qPCR. The circRNA profile and circRNA-associated-ceRNA network in EHI were analyzed with the integrated data of RNA-Seq and miRNA-Seq. Gene ontology (GO) analysis was used to annotate the circRNAs involved in the circRNA-associated-ceRNA networks.ResultsA total of 1365 circRNAs, 30 miRNAs, and 2049 mRNAs were differentially expressed between HARRT-naive EHI patients and HCs. A ceRNA network was constructed with 516 DE circRNAs and 903 DE mRNAs that shared miR response elements with 21 DE miRNAs. GO analysis demonstrated the multiple roles of the circRNAs enriched in EHI with circRNA-associated-ceRNA networks, such as immune response, inflammatory response and defense responses to virus, 67 circRNAs were revealed to be potentially involved in HIV-1 replication through regulating the expression of CCNK, CDKN1A and IL-15.ConclusionsThis study, for the first time, revealed a large circRNA profile and complex pathogenesis roles of circRNAs in EHI. A group of enriched circRNAs and associated circRNA-associated-ceRNA networks might contribute to HIV replication regulation and provide novel potential targets for both the pathogenesis of EHI and antiviral therapy.Electronic supplementary materialThe online version of this article (10.1186/s12967-018-1706-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 58%

Crosstalk in competing endogenous RNA networks reveals new circular RNAs involved in the pathogenesis of early HIV infection

Zhang

et al. 2018

J Transl Med

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“…In this study, we investigated circRNA expression profiles in ecEM compared with euEM of endometriosis patients and identified 294 differentially expressed circRNAs, 146 upregulated and 148 downregulated. Most of those dysregulated circRNAs originate from exons or introns which are in accordance with previous researches (Shen et al, ; Xu et al, , ; Zhang, Ren, Xiao, Xia, & Fang, ). It has been reported that both exonic and intronic circRNAs may exert potential functions in gene regulation (Greene et al, ).…”

Section: Discussionsupporting

confidence: 89%

Circular RNA expression profiles and bioinformatics analysis in ovarian endometriosis

Wang

Luo

Wang

et al. 2019

Molec Gen & Gen Med

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Background Circular RNAs (circRNAs) with miRNA response elements (MREs) could function as competing endogenous RNA (ceRNA) in regulating gene expression, thus playing vital roles in pathogenesis and progression of many diseases. However, the function of circRNAs in endometriosis remains unknown. This study was carried to profile the expression patterns of circRNAs in ovarian endometriosis. Methods High throughput RNA‐Seq was performed in six paired ectopic and eutopic endometrium tissues (ecEM vs. euEM), followed by quantitative real‐time polymerase chain reaction (qRT‐PCR) in 30 paired samples. Through bioinformatics prediction, we constructed a circRNA‐miRNA ‐mRNA network and elucidated circRNAs functions by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Results A total of 146 upregulated and 148 downregulated circRNAs were identified, binding with 2,495 MREs. The qRT‐PCR validation results of four upregulated circRNAs matched the RNA‐Seq data. The ceRNA network included 48 miRNAs and 296 mRNAs. Functional analysis revealed several important pathways such as MAPK signaling pathway, and PI3K‐AKT signaling pathway, which might be associated with the pathogenesis and development of endometriosis. Conclusion Our data suggested that circRNAs are differentially expressed in endometriosis, which might be candidate factors for pathogenesis of this disease and be considered as promising therapeutic targets in the future.

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“…In recent years, circRNAs have been the focus of international research, and a large number of circRNAs have been successfully identified in various cell lines and species [19–22]. Furthermore, studies have demonstrated that circRNAs are expressed in eutopic and normal endometrium [23], endometriosis [24], and endometrial cancer [25]. However, there is currently no information available on the expression and function of circRNAs in the developing normal endometrium, or on the differences in circRNAs expression between the RE and PE.…”

Section: Introductionmentioning

confidence: 99%

Analyses of circRNA profiling during the development from pre-receptive to receptive phases in the goat endometrium

Song

Zhang

Liu

et al. 2019

J Animal Sci Biotechnol

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Background Recent studies have revealed that noncoding RNAs play important regulatory roles in the formation of endometrial receptivity. Circular RNAs (circRNAs) are a universally expressed noncoding RNA species that have been recently proposed to act as miRNA sponges that directly regulate expression of target genes or parental genes. Results We used Illumina Solexa technology to analyze the expression profiles of circRNAs in the endometrium from three goats at gestational day 5 (pre-receptive endometrium, PE) and three goats at gestational day 15 (receptive endometrium, RE). Overall, 21,813 circRNAs were identified, of which 5,925 circRNAs were specific to the RE and 9,078 were specific to the PE, which suggested high stage-specificity. Further analysis found 334 differentially expressed circRNAs in the RE compared with PE ( P < 0.05). The analysis of the circRNA-miRNA interaction network further supported the idea that circRNAs act as miRNA sponges to regulate gene expression. Moreover, some circRNAs were regulated by estrogen (E2)/progesterone (P4) in endometrial epithelium cell lines (EECs) and endometrial stromal cell line (ESCs), and each circRNA molecule exhibited unique regulation characteristics with respect to E2 and P4. Conclusions These data provide an endometrium circRNA expression atlas corresponding to the biology of the goat receptive endometrium during embryo implantation. Electronic supplementary material The online version of this article (10.1186/s40104-019-0339-4) contains supplementary material, which is available to authorized users.

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Circular RNA Expression in Ovarian Endometriosis

Abstract: CircRNAs are differentially expressed in endometriosis, which might be related with pathogenesis of endometriosis.

Cited by 24 publications

References 40 publications

Crosstalk in competing endogenous RNA networks reveals new circular RNAs involved in the pathogenesis of early HIV infection

Crosstalk in competing endogenous RNA networks reveals new circular RNAs involved in the pathogenesis of early HIV infection

Circular RNA expression profiles and bioinformatics analysis in ovarian endometriosis

Analyses of circRNA profiling during the development from pre-receptive to receptive phases in the goat endometrium

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