Circular RNA detection methods: A minireview

Zhang, Mi; Chen, Zhongqiang; Jiang, Caiyun; Liu, Yanan; Wu, Jianhua; Lv, Liang

doi:10.1016/j.talanta.2021.123066

Cited by 38 publications

(22 citation statements)

References 79 publications

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“…This section introduces some traditional circRNAs detection techniques and emerging technical methods ( Table 1 ). Northern blot, qRT-PCR, RNA-seq, and Microarrays are examples of traditional circRNA detection techniques ( 63 ). However, previous RNA analysis methods are also difficult to study circRNAs.…”

Section: Circrnasmentioning

confidence: 99%

Circular RNA and Its Roles in the Occurrence, Development, Diagnosis of Cancer

Zhang

et al. 2022

Front. Oncol.

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Circular RNAs (circRNAs) are non-coding single-stranded covalently closed circular RNA, mainly produced by reverse splicing of exons of precursor mRNAs (pre-mRNAs). The characteristics of high abundance, strong specificity, and good stability of circRNAs have been discovered. A large number of studies have reported its various functions and mechanisms in biological events, such as the occurrence and development of cancer. In this review, we focus on the classification, characterization, biogenesis, functions of circRNAs, and the latest advances in cancer research. The development of circRNAs as biomarkers in cancer diagnosis and treatment also provides new ideas for studying circRNAs research.

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Section: Circrnasmentioning

confidence: 99%

Circular RNA and Its Roles in the Occurrence, Development, Diagnosis of Cancer

Zhang

et al. 2022

Front. Oncol.

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“…At present, the detection process adopted by several studies is as follows: Total RNA extraction, RNA quality inspection and finally reverse transcription (RT) into cDNA. Primers are designed according to the circRNAs requiring to be detected and the expression level of circRNAs are detected by RT-quantitative PCR ( 27 ).…”

Section: Lab/experimental Protocols For the Detection Of Circrnasmentioning

confidence: 99%

Role of circular RNAs in the diagnosis, regulation of drug resistance and prognosis of lung cancer (Review)

Sang

Rao

et al. 2022

Oncol Lett

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Lung cancer is one of the most common malignant tumors in China and is the highest cause of mortality among male and female patients, both in urban and rural areas. A subset of patients with lung cancer only display chest tightness without any other obvious symptoms. This is because most symptoms do not manifest during the early stages of disease development. Consequently, most patients with lung cancer are diagnosed when the disease is in the advanced stages, when they are already unfit for surgical treatment. Furthermore, the prognosis of patients with lung cancer is poor. The 5-year survival rate of patients with stage IA lung cancer is 85%, compared with 6% in those with stage IV. This requires the development of strategies for early diagnosis, treatment and prognosis to improve the management of lung cancer. Circular RNAs (circRNAs) belong to a class of closed circular non-coding RNAs formed by reverse splicing of a precursor mRNA. These RNAs are highly stable, ubiquitously expressed, conserved, and show high specificity. CircRNAs regulate biological processes, such as the proliferation, differentiation and invasion of lung cancer cells. Therefore, they can be used as biomarkers for the early diagnosis and prognosis prediction of lung cancer, as well as novel targets for therapy design. In the present review, the biological characteristics and functions of circRNAs, as well as their application in the diagnosis, control of drug resistance and effect on the prognosis of patients with lung cancer, will be discussed. Contents1. Introduction 2. Classification and characteristics of circRNAs 3. Biological characteristics of circRNAs 4. Biological functions of circRNAs 5. Lab/experimental protocols for the detection of circRNAs 6. Diagnostic significance of circRNAs in lung cancer 7. CircRNAs and resistance to adjuvant therapy in lung cancer 8. CircRNAs and the prognosis of patients with lung cancer 9. Limitations of circRNAs as diagnostic markers for lung cancer 10. Conclusion

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“…RNA sequencing is currently the most widely used method in circRNA research. Sequencing technology, which is different from microarrays, allows for the discovery of novel circRNAs that have not been previously identified by cloning or sequencing and reveals the actual structure of circRNAs [ 39 ]. However, due to their circular nature and extensive sequence overlapping with cognate linear transcripts, there are some specific challenges in detection and quantification of circRNAs, ranging from the initial RNA library establishment to the high algorithmic sensitivity required with low read counts in computational workflows.…”

Section: Research Approaches For Circrnasmentioning

confidence: 99%

Biological functions, mechanisms, and clinical significance of circular RNA in pancreatic cancer: a promising rising star

Chen

Shen

et al. 2022

Cell Biosci

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Pancreatic cancer (PC) is a highly malignant solid tumor with insidious onset and easy early metastasis. Despite tremendous efforts devoted to research in this field, the mechanisms underlying PC tumorigenesis and progression remain unclear. Additionally, robust biomarkers and satisfactory therapeutic strategies for clinical use in PC patients are still lacking. Circular RNAs (circRNAs) are a new type of non-coding RNA originating from precursor messenger RNAs, with a covalent continuous closed-loop structure, strong stability and high specificity. Accumulating evidence suggests that circRNAs may participate in PC development and progression. Abnormal expression of circRNAs in PC is considered a vital factor that affects tumor cell proliferation, migration, invasion, apoptosis, angiogenesis and drug resistance. In this review of relevant articles published in recent years, we describe the basic knowledge concerning circRNAs, including their classification, biogenesis, functions and research approaches. Moreover, the biological roles and clinical significance of circRNAs related to PC are discussed. Finally, we note the questions remaining from recent studies and anticipate that further investigations will address these gaps in knowledge in this field. In conclusion, we expect to provide insights into circRNAs as potential targets for specific PC diagnosis and treatment in the future.

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Circular RNA detection methods: A minireview

Cited by 38 publications

References 79 publications

Circular RNA and Its Roles in the Occurrence, Development, Diagnosis of Cancer

Circular RNA and Its Roles in the Occurrence, Development, Diagnosis of Cancer

Role of circular RNAs in the diagnosis, regulation of drug resistance and prognosis of lung cancer (Review)

Biological functions, mechanisms, and clinical significance of circular RNA in pancreatic cancer: a promising rising star

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