Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

Zhang, Zhen; Fan, Yixuan; Deng, Kaiping; Liang, Yaxu; Zhang, Guomin; Gao, Xiaoxiao; El-Samahy, M.A.; Deng, Mingtian; Wang, Feng

doi:10.1096/fj.202101317r

Cited by 14 publications

(16 citation statements)

References 49 publications

(101 reference statements)

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“…Several reports have revealed ncRNA function in skeletal muscle development and disorder in animals providing novel biomarkers or targets for improving muscular abilities and therapeutic strategies 5,6,24 . Of which, miRNAs could binding to target mRNAs which results in target mRNA degradation or protein translation inhibition, regulating skeletal muscle development via a variety of molecular factors such as myoblast proliferation, differentiation, and apoptosis 4,5,25–28 . In this study, we identified 1208 miRNAs through transcriptome sequencing to reveal the processes by which miRNAs are involved in the regulation of muscle development, of which 313 were differentially expressed between fetus and lamb muscle tissues.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

et al. 2022

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MicroRNAs (miRNAs) are evolutionarily conserved endogenous small non‐coding RNAs that play critical roles in skeletal muscle development. In this study, we identified putative miRNAs that were differentially expressed in the longissimus dorsi muscle between fetus (75 days of pregnancy) and lamb (1 day of age). We detected 1208 miRNAs, 313 of which were differentially expressed. In particular, we found that miR‐145‐5p was differentially and highly expressed in lamb skeletal muscle. In addition, our results demonstrated that overexpression of miR‐145‐5p inhibited the differentiation and apoptosis of goat primary myoblasts (GPMs), whereas knockdown of miR‐145‐5p had the opposite effect. The coding domain sequence (CDS) of ubiquitin‐specific peptidase 13 (USP13) was predicted and validated as a target of miR‐145‐5p. We also demonstrated that the influence as a key regulator of GPMs differentiation is primarily mediated by targeting and inhibiting USP13. Taken together, these results revealed a novel pathway in skeletal muscle development in which miR‐145‐5p targets CDS region of USP13 to regulate differentiation and apoptosis of GPMs.

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Section: Discussionmentioning

confidence: 99%

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

et al. 2022

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“…For example, circRNA generated from circSF1 and the protein it encodes have been shown to extend the lifespan of flies ( Baumann, 2020 ). CircUSP13 promotes goat myogenic cell differentiation and inhibits apoptosis by targeting IGF1 through sponging miR-29c ( Zhang et al, 2022c ), whereas some other circRNAs are involved in the regulation of cashmere fineness ( Zheng et al, 2020 ). Numerous studies have indicated that circRNA-derived specific miRNA regulates the reproductive process.…”

Section: Introductionmentioning

confidence: 99%

Characterization of circular RNA profiles of oviduct reveal the potential mechanism in prolificacy trait of goat in the estrus cycle

et al. 2022

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The mammalian oviduct is functionally highly diverse during the estrus cycle. It provides a suitable milieu for oocyte maturation, sperm capacitation, fertilization, early embryo development and transportation. While there have been many studies of molecular mechanisms on the kidding number of goats, a systematic analysis by which the underlying circular RNAs (circRNAs) changes in the oviduct related to prolificacy traits is lacking. Herein, we present a comprehensive circRNA atlas of the oviduct among high-and low-fecundity goats in the follicular phase (FH vs. FL), luteal phase (LH vs. LL), and estrus cycle (FH vs. LH; FL vs. LL) to unravel their potential regulatory mechanisms in improving kidding number. We generated RNA sequencing data, and identified 4,078 circRNAs from twenty sampled Yunshang black goats. Many of these circRNAs are exon-derived and differentially expressed between each comparison group. Subsequently, eight differentially expressed (DE) circRNAs were validated by RT-qPCR, which was consistent with the RNA-seq data. GO and KEGG enrichment analyses suggested that numerous host genes of DE circRNAs were involved in the hormone secretion, gamete production, fertilization, and embryo development processes. The competing endogenous RNA (ceRNA) interaction network analysis revealed that 2,673 circRNA-miRNA-mRNA axes (including 15 DE circRNAs, 14 miRNAs, and 1,699 mRNAs) were formed, and several target genes derived from the ceRNA network were associated with oviduct functions and reproduction, including SMAD1, BMPR1B, IGF1, REV1, and BMP2K. Furthermore, might play important roles in reproduction. Finally, a novel circRNA, circIQCG, was identified as potentially involved in embryo development. Overall, our study provides a resource of circRNAs to understand the oviductal function and its connection to prolificacy trait of goats in the differentiation estrus cycle.

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“…MiR-100 inhibits IGF1R/PI3K/AKT signaling in bovine skeletal muscle cells, resulting in reduced differentiation and protein expression (22). MiR-29c was sponged by circUSP13, which increased IGF1 expression and up-regulated MYOG and MYHC expression, promoting differentiation of goat primary myoblasts and inhibiting apoptosis (23).…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

Zhao

Xue

et al. 2022

Front. Vet. Sci.

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Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.

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Circular RNA circUSP13 sponges miR‐29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1

Cited by 14 publications

References 49 publications

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

MicroRNA profiling reveals miR‐145‐5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13

Characterization of circular RNA profiles of oviduct reveal the potential mechanism in prolificacy trait of goat in the estrus cycle

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

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