Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis

Abstract

Background: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck. LSCC patients have seriously impaired vocal, respiratory, and swallowing functions with poor prognosis. Circular RNA (circRNA) has attracted great attention in cancer research. However, the expression patterns and roles of circRNAs in LSCC remain largely unknown. Methods: RNA sequencing was performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger sequencing, and FISH were undertaken to study the expression, localization, and clinical significance of circCORO1C in LSCC tissues and cells. The functions of circCORO1C in LSCC were investigated by RNAi-mediated knockdown, proliferation analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis analysis. The regulatory mechanisms among circCORO1C, let-7c-5p, and PBX3 were investigated by luciferase assay, RNA immunoprecipitation, western blotting, and immunohistochemistry.

Circular RNA ZNF609 promotes the malignant progression of glioma by regulating miR-1224-3p/PLK1 signaling

“…Meanwhile, it is characterized by insensitive to conventional chemo-radiotherapy [28,29]. CircRNA is a class of noncoding RNA generated from a non-canonical back splicing process, from a covalent bond between 5' and 3' ends of a single-stranded RNA [30][31][32].…”

Crosstalk between RNA m6A Modification and Non-coding RNA Contributes to Cancer Growth and Progression

“… 69 , 70 In human cancer, circRNA regulates critical cellular processes, including proliferation, metastasis, differentiation, autophagy, and drug resistance. 71 , 72 , 73 , 74 …”

Research Progress of circRNAs in Head and Neck Cancers

“…Multiple circRNAs have been demonstrated to be involved in HNC development through the regulation of the proliferation process ( Figure 1 ). CircATRNL1 ( 40 ), hsa_circ_0007059 ( 41 ), circRNA_002178 ( 42 ), hsa_circ_0046263 ( 43 ), hsa_circ_0005379 ( 44 ), circUHRF1 ( 45 ), circPVT1 ( 46 , 47 ), circHIPK3 ( 48 , 49 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), circDOCK1 ( 52 ), hsa_circ_0055538 ( 53 ), circITCH ( 54 ), hsa_circRNA_101036 ( 55 ), circCCND1 ( 20 ), circMYLK ( 56 ), CDR1as ( 17 , 57 , 58 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circMDM2 ( 63 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 , 67 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circ_0000218 ( 75 ), circ_0001971 ( 76 ), circCORO1C ( 77 ), hsa_circ_0109291 ( 78 ), hsa_circ_0036722 ( 34 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are associated with tumor proliferation ( Table 1 ).…”

“…CircRNAs in HNC have important roles in HNC metastasis and invasion ( Figure 2 ). Hsa_circ_0007059 ( 41 ), circUHRF1 ( 45 ), circHIPK3 ( 48 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), hsa_circ_0055538 ( 53 ), CDR1as ( 17 , 57 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circCORO1C ( 77 ), hsa_circ_0004491 ( 37 ), circ_0001971 ( 33 ), circFLNA ( 99 ), hsa_circ_0001162 ( 100 ), circARHGAP12 ( 101 ), circCRIM1 ( 102 ), circEPSTI1 ( 103 ), hsa_circ_0109291 ( 78 ), circRNA_002178 ( 42 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are involved in the regulation of HNC invasion and metastasis ( Table 2 ).…”

“…Further studies have shown that miR-9 can bind to circRNA_000543, and a bioinformatics software predicts that miR-9 can bind to the 3′ UTR of the PDFGRB. Therefore, the author finally proved that circRNA_000543 is involved in radiation resistance of NPC by blocking inhibition of miR-9 to PDFGRB ( 77 ).…”