Circular RNA circBFAR promotes glioblastoma progression by regulating a miR‐548b/FoxM1 axis

Abstract

Glioblastoma multiforme (GBM) is the most common and aggressive type of tumor of the primary nervous system. Treatment options for GBM include surgery, chemotherapy, and radiation therapy; however, the clinical outcomes are poor, with a high rate of recurrence. An increasing number of studies have shown that circular RNAs (circRNAs) serve important roles in several types of cancer. Gene Expression Omnibus (GEO) database was utilized to identify the differentially expressed cir-cRNAs and their biological functions. Then, we detected the circular RNA bifunctional apoptosis regulator (circBFAR) was significantly increased in three GEO datasets. However, the role of circBFAR has not been reported in GBM. In this study, the expression of circBFAR was significantly increased both in GBM tissues or cell lines and was negatively correlated with overall survival in patients with GBM. Knockdown of circBFAR inhibited proliferation and invasion both in vitro and in vivo. Increased expression of circBFAR resulted in a reduction of miR-548b expression in glioma cells. A luciferase reporter and RIP assay indicated that miR-548b was a direct target of circBFAR, and miR-548b may negatively regulate the expression of FoxM1. Rescue experiments showed that overexpression of FoxM1 could counter the effect of circBFAR silencing on the proliferation and invasion of glioma cell lines. Moreover, we identified that circBFAR regulates FoxM1 by

An Integrated Study on the Differential Expression of the FOX Gene Family in Cancer and Their Response to Chemotherapy Drugs

“…Compared to normal tissues, FOXM1 expressed at a higher level in GBM ( Figure 3 B). A circBFAR/miR−548b/ FOXM1 axis [ 51 ] and a FOXM1 /ADAM17 feedback loop [ 52 ] have been identified as involved in the development of GBM. In this light, FOXM1 might serve as a potential target for GBM.…”

CircCCDC66: Emerging roles and potential clinical values in malignant tumors

“…Co-transfection of miR-320a or forkhead box M1 (FOXM1) plasmids into glioma cells could rescue the consequences of si-circCCDC66-induced cell growth inhibition to some extent. Importantly, FOXM1 was identified as a key target of circCCDC66 involved in regulating DNA damage response pathways ( 89 ). This highlights the fact that circCCDC66 is overexpressed in gliomas and acts as an oncogene.…”

CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis

“…Circ_0000218 was up‐regulated in LSCC cells and negatively regulated the expression of miR‐139‐3p to promote LSCC cell viability and inhibit apoptosis 9 . Of note, previous studies suggested that circBFAR frequently acted as a carcinogenic factor by regulating cell proliferation, invasion, and glycolysis in diverse tumors 10,11 . However, the role and mechanism of circBFAR in the development of LSCC have not been reported.…”

“… 9 Of note, previous studies suggested that circBFAR frequently acted as a carcinogenic factor by regulating cell proliferation, invasion, and glycolysis in diverse tumors. 10 , 11 However, the role and mechanism of circBFAR in the development of LSCC have not been reported.…”