Circular RNA circANKS1B acts as a sponge for miR‐152‐3p and promotes prostate cancer progression by upregulating TGF‐α expression

Abstract

Background A growing number of studies indicate that circular RNAs (circRNAs) play critical roles in human diseases, and show great potential as biomarkers and therapeutic targets. This study aimed to investigate the expression and function of circANKS1B in prostate cancer (PC). Methods The expression of circANKS1B and miR‐152‐3p was analyzed by real‐time quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR). Cell migration and invasion were measured using a transwell assay. The interaction between circANKS1B and miR‐152‐3p was confirmed by a dual‐luciferase reporter gene assay. Rescue experiments were conducted to determine whether circANKS1B regulated the invasion of PC cells via the circANKS1B‐miR‐152‐3p‐TGF‐α pathway. Results The expression of circANKS1B was markedly upregulated in both PC cells and tissues. Moreover, high circANKS1B expression was associated with poor prognosis in PC patients. Dual‐luciferase reporter assay indicated that circANKS1B directly bound to miR‐152‐3p. Furthermore, circANKS1B negatively regulated miR‐152‐3p expression. Knockdown of circANKS1B markedly suppressed cell migration and invasion and TGF‐α expression in PC cells, whereas the effects of circANKS1B silencing were reversed by miR‐152‐3p deficiency. In addition, the impact of miR‐152‐3p silencing on invasion of circANKS1B‐deficient PC cells was also abrogated by TGF‐α deficiency. Overall, circANKS1B acts as a sponge for miR‐152‐3p to promote PC progression by upregulating TGF‐α expression. Conclusion Our findings reveal that circANKS1B may be a potential prognostic biomarker and therapeutic target for PC.

Regulation of transforming growth factor-beta1 by circANKS1B/miR-515-5p affects the metastatic potential and cisplatin resistance in oral squamous cell carcinoma

“…Moreover, targeting TGF-β increases chemosensitivity of cisplatin-resistant OSCC cells [ 5 ]. Noticeably, previous studies have mainly focused on the function of circANKS1B in the metastatic potential of breast cancer [ 18 ], colorectal cancer [ 19 ] and prostate cancer [ 17 ]. Based on the correlation between circANKS1B and TGF-β in OSCC, we first investigated the role of circANKS1B in cisplatin resistance.…”

“…For instance, circPKD2 affects OSCC progression by sponging the miR-204-3p/ adenomatous polyposis coli 2 (APC2) axis [ 16 ]. Among them, CircANKS1B (circ_0007294) is a known circRNA originating from exons 5 to 8 of the ANKS1B gene and has drawn more attention because of its key roles in several cancers [ 17 ]. In triple-negative breast cancer (TNBC), high expression of circANKS1B is closely associated with lymph node metastasis and may act as an independent risk factor for breast cancer patients [ 18 ].…”

Hsa_circRNA_100146 Promotes Prostate Cancer Progression by Upregulating TRIP13 via Sponging miR-615-5p

“…Over the past years, increasing circRNAs have been reported to play a role in regulating PCa occurrence and development. For instance, high expression of circular RNA circANKS1B in PCa was reported, and its knockdown suppressed the proliferation and metastasis of PCa cells by upregulating TGF-α expression via sponging miR-152-3p ( Tao et al, 2021 ). Circ_0006404 was shown to exhibit a higher level in PCa specimens than non-tumor specimens.…”

Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

“…Moreover, recent studies have evaluated the role of a circular RNA, known as circANKS1B, as a potential prognostic biomarker and therapeutic target for PC. This circANKS1B has been suggested to act as a sponge for miR-152-3p, promoting PC progression by upregulating TGF-α expression [4]. Others include the role of miR-144 as a potential biomarker for predicting PC progression, by its interaction with EZH2, reducing cell viability and promoting cell apoptosis [5], or the role described for miR-145-5p in PC metastasis by binding TOP2A, which is indicated as a useful biomarker for metastatic PC detection [2].…”