Hsa_circRNA_100146 Promotes Prostate Cancer Progression by Upregulating TRIP13 via Sponging miR-615-5p

Abstract

Objective: This study was conducted for investigating the functions of circular RNA circRNA_100146 (circRNA_100146) in the development of prostate cancer (PCa) and identifying the underlying mechanisms of the circRNA_100146/miR-615-5p/TRIP13 axis.Materials and Methods: Under the support of RT-PCR, the expression of circRNA_100146 in PCa cells was examined. Cell Counting Kit-8 (CCK-8) assays and clone formation assays were applied to the assessment of cell proliferation. We then determined cell invasion and migration through transwell assays and wound healing assays. RNA pull-down assays and luciferase reporter assays were performed for the exploration of the regulatory effects of potential molecules on the expressions of the targeting genes. In addition, a nude mouse xenograft model was applied to demonstrate the oncogenic roles of circRNA_100146 in PCa.Results: CircRNA_100146 expression was distinctly upregulated in PCa cells. Silencing of circRNA_100146 suppressed PCa cells’ invasion, migration, and proliferation. CircRNA_100146 sponged miR-615-5p to suppress its expressions, while miR-615-5p targeted the 3’-UTR of TRIP13 to repress the expression of TRIP13. In addition, we observed that knockdown of miR-615-5p reversed the suppression of circRNA_100146 silence on the proliferation and invasion of PCa cells. In addition, the tumor growth was also suppressed by silencing circRNA_100146 in vivo.Conclusion: CircRNA_100146 is a tumor promoter in PCa, which promoted progression by mediating the miR-615-5p/TRIP13. CircRNA_100146 can be a potential candidate for targeted therapy of PCa.

Circular RNA circ_0001459 accelerates hepatocellular carcinoma progression via the miR‐6165/IGF1R axis

“…miR‐6165 can interact directly with IGF1R by binding to the 3ʹ‐UTR of IGF1R. Previous studies have shown that circRNAs, as miRNA sponges, regulate the expression of tumor regulatory genes through the circRNA–miRNA–mRNA axis 17–19 . In our study, we found that circ_0001459 sponged miR‐6165 to regulate the expression of IGF1R in HCC.…”

“…For example, circ_0000337 promotes the cisplatin resistance of esophageal cancer via the miR‐377‐3p/JAK2 pathway, which might be a potential therapeutic target of esophageal cancer 17 . Circ_100146 acts as a tumor promoter in prostate cancer via the miR‐615‐5p/TRIP13 axis 18 . Circ_0089823 regulates SOX4 by sponging miR‐507, miR‐557, miR‐579‐3p, and miR‐1287‐5p, thereby enhancing the progression of non‐small‐cell lung cancer 19 .…”

“… 17 Circ_100146 acts as a tumor promoter in prostate cancer via the miR‐615‐5p/TRIP13 axis. 18 Circ_0089823 regulates SOX4 by sponging miR‐507, miR‐557, miR‐579‐3p, and miR‐1287‐5p, thereby enhancing the progression of non‐small‐cell lung cancer. 19 Additionally, circRNAs are associated with HCC.…”

CircRNA hsa_circ_0018289 exerts an oncogenic role in cervical cancer progression through miR‐1294/ICMT axis

“…CircRNA circGSE1 accelerated CC cell migration and invasion by acting as a sponge of miR‐138‐5p and increasing Vimentin expression level 28 . Circ‐CCDC66 could facilitate the malignant properties of CC cells by sponging miR‐452‐5p to enhance REXO1 expression 29 . Enforced expression of circHIPK3 promoted growth, migration, and invasion of CC cells by modulating the miR‐485‐3p/FGF2 axis 30 .…”

“… 28 Circ‐CCDC66 could facilitate the malignant properties of CC cells by sponging miR‐452‐5p to enhance REXO1 expression. 29 Enforced expression of circHIPK3 promoted growth, migration, and invasion of CC cells by modulating the miR‐485‐3p/FGF2 axis. 30 He et al reported that hsa_circ_0018289 was positively correlated with the tumor size, lymph node metastasis, and stage of CC patients, suggesting that it was a promising biomarker for CC prognosis and disease monitoring.…”