Circular RNA circ_0062389 modulates papillary thyroid carcinoma progression via the miR-1179/high mobility group box 1 axis

Abstract

Circular RNAs (circRNAs) feature prominently in regulating the progression of tumors, including papillary thyroid carcinoma (PTC). This work is designated to delve into the role of circ_0062389 in PTC. Generally, quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect circ_0062389, miR-1179 and high mobility group box 1 (HMGB1) mRNA expression levels. RNase R assay was used to verify the circular characteristics of circ_0062389. After circ_0062389 was knocked down in PTC cells, CCK-8 assay was adopted to determine cell viability. Wound healing assay was leveraged to probe cell migration. Besides, Western blot assay was executed to examine the expression levels of HMGB1 and epithelial-mesenchymal transformation (EMT)-related markers (E-cadherin and N-cadherin). Dual-luciferase reporter assay was performed to authenticate the targeting relationships between miR-1179 and circ_0062389, as well as miR-1179 and HMGB1. Here, this work proved that circ_0062389 was greatly up-regulated in PTC tissues and cell lines. The high expression of circ_0062389 was related to large tumor size and positive lymphatic metastasis. Knocking down circ_0062389 could inhibit the proliferation, migration and EMT process of PTC cells. Besides, miR-1179 was a downstream molecule of circ_0062389. Furthermore, miR-1179 inhibitors could partially reverse the above effect of knocking down circ_0062389 on PTC cells. It was also confirmed that HMGB1 was a direct target of miR-1179 and mediated the effects of circ_0062389 and miR-1179 in PTC. Altogether, circ_0062389 can adsorb miR-1179, and regulate HMGB1 expression, thus playing a role in PTC.

RETRACTED ARTICLE: Long non-coding RNA DCST1-AS1/hsa-miR-582-5p/HMGB1 axis regulates colorectal cancer progression

“…In CRC patients, HMGB1 expression is also proposed as a prognostic marker related to CRC patient survival [ 31 , 32 ]. A recent study revealed that HMGB1 is also implicated in papillary thyroid carcinoma progression [ 33 ]. However, the mechanisms regulating HMGB1 expression in CRC remains to be elucidated.…”

Circ_0002111 modulates the growth process of papillary thyroid carcinoma cells by targeting the miR-363-3p/HMGB1 axis

“…During tumor development, HMGB1 works as both a pro-tumor and antitumor protein by facilitating or arresting tumor growth, proliferation, angiogenesis and invasion [26]. HMGB1 was confirmed to engage in the modulation of circ_0062389/miR-1179/HMGB1 axis in PTC progression as a target gene of miR-1179 [49]. As predicted in this experiment, HMGB1 was learned to be a downstream gene of miR-363-3p.…”

Circ_LDLR promotes the progression of papillary thyroid carcinoma by regulating miR‐1294/HMGB3 axis

“…CircRNAs have been suggested to modulate downstream miRNA levels through molecular "sponge effect," thus inhibiting the malignant progression of cancer. [5][6][7][8] We have identified that miR-1294 was one the targets of circ_LDLR. Therefor, we tried to explore whether circ_LDLR promoted PTC cells by regulating miR-1294.…”

“…Circular RNAs (circRNAs) are class of noncoding RNAs (ncRNAs) that have closed‐loop architecture, which are suggested to be the critical factors regulating carcinogenesis, including thyroid cancer. [ 5,6 ] CircRNAs can regulate a series of cellular processes involved in thyroid cancer pathogenesis, such as apoptosis, angiogenesis, tumor growth and invasion, through microRNA (miRNA) sponging action. [ 7,8 ] In PTC, many circular RNAs have been identified.…”