Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198

Zhong, Jin-xiu; Kong, Yun-Yuan; Luo, Rong-guang; Xia, Guomin; He, Wenxing; Chen, Xuezhong; Tan, Weiwei; Chen, Qingjie; Huang, Yu-yin; Guan, Yihui

doi:10.1186/s12967-021-03114-8

Cited by 18 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Radioimmunoprecipitation assay buffer (Beyotime, Shanghai, China) was performed for extracting total proteins from cells, and then, 50 μ g proteins were used for protein analysis as previously depicted [ 16 ]. The incubation of primary antibody was at 4°C overnight and secondary antibody (Abcam, Cambridge, MA, USA; ab205718, 1 : 5000) was at 25°C for 1 h. The primary antibodies were shown as follows: anti-proliferating cell nuclear antigen (anti-PCNA; Abcam, ab92552, 1 : 1000), anti-Bcl-2 associated X (anti-Bax; ab53154, 1 : 1000), anti-YAP1 (Abcam, ab52771, 1 : 1000), anti-P65 (Abcam, ab16502, 1 : 1000), anti-phospho-P65 (anti-p-P65; Abcam, ab86299, 1 : 1000), anti-inhibitor of nuclear factor- κ B (anti-I κ B α ; CST, Boston, MA, USA, #4812, 1 : 1000), anti-phospho-I κ B α (anti-p-I κ B α ; CST, #2859, 1 : 1000), and anti-GAPDH (Abcam, ab9485, 1 : 3000).…”

Section: Methodsmentioning

confidence: 99%

circ_0041795 Induces YAP1 Upregulation to Accelerate the Progression of Diabetic Retinopathy through Binding to miR-589-5p

Sun

Liú

Wei

2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

Background. Circular RNAs (circRNAs) are involved in the pathogenesis of many diseases, and circ_0041795 was shown to promote the progression of diabetic retinopathy (DR). The aim of this study was to explore the molecular mechanism of circ_0041795 in DR. Methods. Human retinal pigment epithelial cells ARPE-19 were treated with high glucose (HG). circ_0041795, miR-589-5p, and Yes-associated protein 1 (YAP1) levels were measured by reverse transcription-quantitative polymerase chain reaction assay. Biological behaviors were examined by Cell Counting Kit-8 assay for cell viability, EdU assay for cell proliferation, flow cytometry for cell apoptosis, and enzyme-linked immunosorbent assay for cell inflammation. Oxidative stress was assessed via the commercial kits. Western blot was performed for analysis of protein expression. The molecular binding was assessed via dual-luciferase reporter assay and pull-down assay. Results. HG-induced inhibiting effects on cell viability and proliferation but promoting effects on cell apoptosis, inflammation, and oxidative stress were ameliorated by silence of circ_0041795. circ_0041795 was identified to act as a miR-589-5p sponge. The regulation of circ_0041795 in HG-induced cell injury was achieved by inhibiting miR-589-5p. miR-589-5p targeted YAP1 and relieved HG-induced cell dysfunction via downregulating YAP1. circ_0041795 sponged miR-589-5p to regulate YAP1 level and activated the NF-κB pathway through the miR-589-5p/YAP1 axis. Conclusion. All these results elucidated that circ_0041795 facilitated the development of DR by inducing miR-589-5p-mediated YAP1 upregulation.

show abstract

Section: Methodsmentioning

confidence: 99%

circ_0041795 Induces YAP1 Upregulation to Accelerate the Progression of Diabetic Retinopathy through Binding to miR-589-5p

Sun

Liú

Wei

2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

show abstract

“…Previous research reported that circRNA is involved in many biological processes through multiple mechanisms [13]. CircRNA could influence cancer cell differentiation, proliferation, migration, protein cracking, and apoptosis based on the function of ceRNA or miRNA "sponge" [14][15][16]. These properties of circRNA allow it to become an ideal tumor biomarker for the diagnosis and therapy of BC cancer.…”

Section: Discussionmentioning

confidence: 99%

Identification of Circular RNA hsa-circ-0006969 as a Novel Biomarker for Breast Cancer

Wang¹,

Li²,

Tian³

et al. 2022

Oncologie

View full text Add to dashboard Cite

Background: To investigate the characteristics of circular RNA hsa-circ-0006969 in breast cancer and identify it as a novel biomarker for breast cancer. Methods: Three breast cancer (BC) patient tissues were selected to perform human circRNA microarray analysis. GeneSpring 13.0 (Agilent) software was applied for analyzing the data. Another 116 BC patients were recruited for verification. Hsa-circ-0006969 was found as a potential circRNA for BC diagnostic biomarker. The structure of hsa-circ-0006969 was predicted by circPrimer1.2 software. MiRanda v3.3, RNA hybrid 2.1, and Cytoscape 3.6.0 were used for predicting the networks of circRNA-miRNA. T-test, Curve regression, and ROC analysis were applied to certify the diagnostic values of hsa-circ-0006969. Using SPSS25.0 software to perform the Statistical analysis. Results: 546 higher expression and 1475 lower expression circRNAs were identified. Four circRNAs were filtrated for further verification. Hsa-circ-0006969 was significantly low expressed in BC tissues and peripheral blood. Hsa-circ-0006969 was confirmed as higher diagnostic correlation with BC tissues (AUC = 0.965) and peripheral blood (AUC = 0.842), with Grade 1 (AUC = 0.639), ER-positive (AUC = 0.612), TNM I (AUC = 0.693), TNM II (AUC = 0.712), TNM III (AUC = 0.757), TNM early stage(I, II) (AUC = 0.709). Hsa-circ-0006969 presented more effective diagnostic values for tumor metastasis (AUC = 0.784) compared with CA153 (AUC = 0.752). Conclusion: Hsa-circ-0006969 could be a novel biomarker for the diagnosis and treatment of BC. KEYWORDSBreast cancer (BC); circular RNA; microarray; biomarker Abbreviations TNM Tumor Node Metastasis HER-2 Human epidermal growth factor receptor-2 CEA Carcino-embryonic antigen This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

show abstract

“…50 μg proteins were used for protein detection by western blot, as previously stated [17]. Radioimmunoprecipitation assay buffer (Sigma) was used for obtaining total proteins, and BCA Protein Assay Kit (Sigma) was exploited to determine protein concentrations.…”

Section: Methodsmentioning

confidence: 99%

Circ_0003146 upregulates SCARB1 expression by acting as a miR-1272 sponge to promote malignant behaviors of clear cell renal cell carcinoma

Xia¹,

Wang

Lü³

2022

Anti-Cancer Drugs

View full text Add to dashboard Cite

Circular RNAs (circRNAs) exhibit essential regulation in the malignant development of clear cell renal cell carcinoma (ccRCC). The aims of this study were to investigate the role and mechanism of circ_0003146 in the biologic behaviors of ccRCC. RNA level analysis was performed through reverse transcription-quantitative PCR assay. Cell proliferation was measured by EdU assay and cell counting kit-8 assay. The protein expression was analyzed using a western blot. Flow cytometry and caspase 3 activity assay were used to assess cell apoptosis. Cell migration and invasion were evaluated via wound healing assay and transwell assay. Circ_0003146 function in vivo was determined by xenograft tumor assay. Dual-luciferase reporter assay was applied for target relation analysis. Circ_0003146 upregulation was detected in ccRCC tissues and cells. Downregulation of circ_0003146 induced inhibition of proliferation, migration, invasion and EMT but the promotion of apoptosis in ccRCC cells. Tumor growth in vivo was inhibited after knockdown of circ_0003146. Circ_0003146 directly interacted with miR-1272, and the miR-1272 sponging effect was responsible for the function of circ_0003146. Scavenger receptor class B type 1 (SCARB1) was a target of miR-1272, and circ_0003146 regulated SCARB1 level by absorbing miR-1272. The regulation of circ_0003146 in ccRCC progression was achieved by upregulating SCARB1 in part. The current findings demonstrated that circ_0003146 contributed to the malignant progression of ccRCC via inducing SCARB1 upregulation by targeting miR-1272.

show abstract

Circular RNA circ-ERBB2 promotes HER2-positive breast cancer progression and metastasis via sponging miR-136-5p and miR-198

Cited by 18 publications

References 41 publications

circ_0041795 Induces YAP1 Upregulation to Accelerate the Progression of Diabetic Retinopathy through Binding to miR-589-5p

circ_0041795 Induces YAP1 Upregulation to Accelerate the Progression of Diabetic Retinopathy through Binding to miR-589-5p

Identification of Circular RNA hsa-circ-0006969 as a Novel Biomarker for Breast Cancer

Circ_0003146 upregulates SCARB1 expression by acting as a miR-1272 sponge to promote malignant behaviors of clear cell renal cell carcinoma

Contact Info

Product

Resources

About