Circular RNA and its potential as prostate cancer biomarkers

Tucker, Dwayne; Zheng, Wei; Zhang, Dahong; Dong, Xuesen

doi:10.5306/wjco.v11.i8.563

Cited by 16 publications

(13 citation statements)

References 69 publications

(146 reference statements)

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“…Further works properly distinguishing EVs from non-vesicular circulating factors by size exclusion chromatography for instance 57 , will address whether some of these extracellular entities contain distinct types of noncoding RNAs. Nevertheless, Our results come as further support of considering these noncoding RNAs as surrogate circulating markers and regulatory elements for prostate cancer progression 58 .…”

Section: Discussionsupporting

confidence: 71%

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

Almeida¹,

Gabriel²,

Firlej³

et al. 2021

Preprint

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Long noncoding (lnc)RNAs modulate gene expression alongside presenting unexpected source of neoantigens. Despite their immense interest, their ability to be transferred and control adjacent cells is unknown. Extracellular Vesicles (EVs) offer a protective environment for nucleic acids, with pro and anti-tumorigenic functions by controlling the immune response. In contrast to extracellular non-vesicular RNA, few studies have addressed the full RNA content within human fluids’ EVs and none have compared them with their tissue of origin. Here, we performed Total RNA-Sequencing on 6 Formaldehyde-Fixed-Parafilm-Embedded (FFPE) prostate cancer (PCa) tumor tissues and their paired urinary (u)EVs to provide the first whole transcriptome comparison from the same patients. UEVs contain simplified transcriptome with intron-free cytoplasmic transcripts and specific lnc/circular (circ)RNAs, strikingly common to all patients. Our full cellular and EVs transcriptome comparison within 3 common PCa cell lines identified a set of overlapping 14 uEV-circRNAs characterized as essential for prostate cell proliferation in vitro and 15 uEV-lncRNAs that we predicted to encode 768 high-affinity neoantigens. Our dual analysis of EVs-lnc/circRNAs both in urines’ and in vitro’s EVs provides a fundamental resource for future uEV-lnc/circRNAs phenotypic characterization involved in PCa.

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Section: Discussionsupporting

confidence: 71%

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

Almeida¹,

Gabriel²,

Firlej³

et al. 2021

Preprint

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show abstract

“…The identification of circRNAs has been complicated by bioinformatics challenges, however the development of improved detection methodologies and statistical methods has aided circRNA research, particularly with improvements in reducing false-positive detection rates (Szabo and Salzman, 2016). In PCa, a number of circRNAs have been identified which have been identified as potential biomarkers (Tucker et al, 2020). Our laboratory previously identified the circRNA, hsa_circ_0004870, to be associated with resistance to the androgen receptor Sawada et al, 2020) *References refer to the previous identification of these genes in cancer.…”

Section: Discussionmentioning

confidence: 99%

Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

Greene

Baird

Lim

et al. 2021

Front. Cell Dev. Biol.

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Circular RNAs (circRNAs), a recently discovered non-coding RNA, have a number of functions including the regulation of miRNA expression. They have been detected in a number of malignancies including prostate cancer (PCa). The differential expression pattern of circRNAs associated with PCa and androgen receptor (AR) status was investigated in this study. circRNA profiling was performed using a high throughout microarray assay on a panel of prostate cell lines, which consisted of normal, benign, and malignant cells (n = 9). circRNAs were more commonly significantly up-regulated (p < 0.05) than downregulated in malignant cell lines (n = 3,409) vs. benign cell lines (n = 2,949). In a grouped analysis based on AR status, there were 2,127 down-regulated circRNAs in androgen independent cell lines compared to 2,236 in androgen dependent cell lines, thus identifying a potential circRNA signature reflective of androgen dependency. Through a bioinformatics approach, the parental genes associated with the top 10 differentially expressed circRNAs were identified such as hsa_circ_0064644, whose predicted parental gene target is RBMS3, and hsa_circ_0060539, whose predicted gene target is SDC4. Furthermore, we identified three circRNAs associated with the parental gene Caprin1 (hsa_circ_0021652, hsa_circ_0000288, and hsa_circ_0021647). Other studies have shown the importance of Caprin1 in PCa cell survival and drug resistance. Given the modified circRNA expression signatures identified here, these hypothesis generating results suggest that circRNAs may serve as potential putative diagnostic and predictive markers in PCa. However, further validation studies are required to assess the true potential of these markers in the clinical setting.

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“…Increasing evidence suggests that circRNAs play a role in cancer pathogenesis (Vo et al, 2019). At present, many researchers have reported that circRNAs can function as markers of prognosis and diagnosis of many cancer types such as prostate cancer (Tucker et al, 2020). Moreover, circRNAs regulate cancer cell growth, metastasis, and drug resistance (Liang et al, 2020;Ma et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p

Deng

Yu²,

Deng

et al. 2020

Front. Cell Dev. Biol.

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Prostate cancer is the most common malignant tumor of the urinary system. The mechanisms of the initiation and progression of prostate cancer have not been fully elucidated. Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer pathogenesis. In this study, we aimed to identify differentially expressed circRNAs in prostate cancer tissues and explored the role of circRNAs in the pathogenesis of prostate cancer. By screening a circRNA microarray assay, we found that circ_0088233 was upregulated in prostate cancer tissues compared to adjacent normal tissues, and this upregulation can be verified in 46 pairs of prostate cancer and adjacent normal tissues examined using quantitative reverse transcription-PCR. The level of circ_0088233 correlated with the TNM stage. Knockdown of circ_0088233 reduced cell proliferation, migration, invasion, and induced G1 phase arrest and apoptosis. In addition, miR-185-3p was identified as the downstream target of circ_0088233 using luciferase reporter assays and a biotinylated circ_0088233 probe pull-down assay. The miR-185-3p level showed a negative correlation with the circ_0088233 level in prostate cancer tissues. Overexpression of circ_0088233 blocked the effects of miR-185-3p on cell proliferation, migration, invasion, cell cycle, and apoptosis. In conclusion, circ_0088233 may function as an oncogene and play an oncogenic role by sponging hsa-miR-185-3p. This study increases the understanding of circRNAs in the progression of prostate cancer. These results implicate circ_0088233 as a potential therapeutic target for prostate cancer.

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Circular RNA and its potential as prostate cancer biomarkers

Cited by 16 publications

References 69 publications

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

Hsa_circ_0088233 Alleviates Proliferation, Migration, and Invasion of Prostate Cancer by Targeting hsa-miR-185-3p

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