Circular RNA 103862 Promotes Proliferation and Invasion of Laryngeal Squamous Cell Carcinoma Cells Through the miR-493-5p/GOLM1 Axis

Wang, Xin; Wu, Tianyi; Wang, Peng; Yang, Like; Li, Qiuying; Wang, Jingting; Zhao, Rui; Zhang, Jiarui; Liu, Ming; Cao, Jing; Tian, Linli; Yu, Boyu; Sun, Yanan

doi:10.3389/fonc.2020.01064

Cited by 26 publications

(25 citation statements)

References 32 publications

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“…Increasing evidence has shown that circRNAs play a crucial role in carcinogenesis, cancer progression, and clinical outcomes of various human cancers ( 19 ). For example, researchers found Circular RNA 103862 promoted proliferation and invasion of laryngeal squamous cell carcinoma cells through the miR-493-5p/GOLM1 axis ( 12 ). Besides, CircRNA_ACAP2 could suppress EMT in head and neck squamous cell carcinoma by targeting the miR-21-5p/STAT3 signalling axis ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

“…Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of human malignancy and involves carcinoma of several anatomic sites, such as lip, oral cavity, pharynx (nasopharynx, oropharynx, hypopharynx), and larynx, with an annual incidence of~600,000 per year (12,13). Although the diagnosis and treatment have advanced in recent years, HNSCC still has a high incidence and mortality rate in developing countries (14,15).…”

Section: Discussionmentioning

confidence: 99%

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The circ_0032822 Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma Cells Through miR-141/EF3 Signaling Axis

Zhang

Han

2021

Front. Oncol.

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Head and neck squamous cell carcinoma (HNSCC) refers to an epithelial malignant tumor that originates in the head and neck, and over 600,000 new cases are reported every year, However, the overall prognosis is still poor due to local recurrence and distant metastasis after surgery. The circ_0032822 has been reported upregulated in human oral squamous cell carcinoma; however, the detailed function or mechanism remains unknown. In this study, we confirmed the upregulation of circ_0032822 in HNSCC tumor tissues. Functionally, the overexpression of circ_0032822 significantly promoted the proliferation of HNSCC cell lines along with the S phase arrest and reduced apoptosis, while downregulation of circ_0032822 has the opposite effect in vitro. Mechanistic analysis showed that circ_0032822 acted as a competing endogenous RNA of miR-141 to diminish the repressive effect of miR-141 on its target E2F3. In conclusion, we demonstrated that circ_0032822 functions as a tumor oncogene in HNSCC and that its function is regulated via the miR-141/E2F3 axis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The circ_0032822 Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma Cells Through miR-141/EF3 Signaling Axis

Zhang

Han

2021

Front. Oncol.

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“…CircRNA_103862 is highly expressed in the tumour tissues of LSCC. CircRNA_103862 regulates the expression of GOLM1 by interacting with miR-493-5p, and thus promotes proliferation and invasion of LSCC cells [16]. CircCORO1C acts as a sponge of let-7c-5p, and regulates its downstream gene PBX3 in LSCC, and CircCORO1C/let-7c-5p/PBX3 axis affects LSCC progression [17].…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0042823 accelerates cancer progression via miR-877-5p/FOXM1 axis in laryngeal squamous cell carcinoma

Sun

et al. 2021

Annals of Medicine

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Background: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumour of the head and neck. Our previous study reveals that the circular RNA (circRNA) hsa_circ_0042823 is abnormally expressed in LSCC, suggesting that hsa_circ_0042823 is closely associated with LSCC. Here, we attempted to explore the molecular mechanism of hsa_circ_0042823 in LSCC. Methods: Quantitative real-time PCR and western blot were performed to assess the expression of gene and protein in human laryngeal carcinoma cells, TU212 and TU686. MTT and transwell assays were performed to examine cell proliferation, migration and invasion. The relationship among hsa_circ_0042823, miR-877-5p and forkhead box M1 (FOXM1) was verified by luciferase reporter assay. Finally, we constructed a subcutaneous tumour mouse model to analyse in vivo growth of LSCC cells following knockdown of hsa_circ_0042823. Results: Compared with normal human bronchial epithelial cells (HBECs), hsa_circ_0042823 was highly expressed in the LSCC cell lines (AMC-HN-8 and TU686). Further studies demonstrated that hsa_circ_0042823 interacted with miR-877-5p, and FOXM1 was the target of miR-877-5p. Hsa_circ_0042823 promoted the expression of FOXM1 via its ceRNA activity on miR-877-5p. Hsa_circ_0042823 overexpression promoted proliferation, migration and invasion of AMC-HN-8 cells through regulating miR-877-5p/FOXM1 axis. Additionally, inhibition of hsa_circ_0042823 inhibited growth of LSCC in vivo via miR-877-5p/FOXM1 axis. Conclusions: Hsa_circ_0042823/miR-877-5p/FOXM1 axis participates in the progression of LSCC. This work demonstrates that hsa_circ_0042823 accelerates cancer progression by regulating miR-877-5p/FOXM1 axis in LSCC. Therefore, this study may provide new insights into the pathogenesis of LSCC. KEY MESSAGESHsa_circ_0042823 promotes FOXM1 expression by sponging miR-877-5p. Hsa_circ_0042823 promotes proliferation, migration, invasion of LSCC cells. Hsa_circ_0042823 knockdown inhibits tumour growth of LSCC via miR-877-5p/FOXM1 axis.

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“…Emerging evidence has suggested that the ceRNA pattern also works in LSCC progression. For example, circRNA_103862 promotes LSCC cell proliferation, migration, and invasion by the miR-493-5p/GOLM1 axis [36], while hsa_circ_0042666 promotes LSCC cell proliferation and invasion by the miR-223/TGFBR3 axis [37]. To further determine the biological functions of circPTK2, we analyzed its ceRNA mechanism in LSCC.…”

Section: Discussionmentioning

confidence: 99%

CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis

Yang

Jin

Chang

2021

Journal of Oncology

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Laryngeal cancer accounts for 20% of all head and neck malignancies. Laryngeal squamous cell carcinoma (LSCC) is the most common type of laryngeal cancer and is characterized by squamous differentiation, a high mortality rate, and poor prognosis. Accumulating studies have indicated that circular RNAs (circRNAs) are critical regulators in many cancers. CircPTK2 exerts an important regulatory role in several cancers. In this study, we aimed to elucidate the function of circPTK2 (hsa_circ_0003221) in LSCC. Through a series of investigations, we discovered that circPTK2 was significantly upregulated in LSCC tissues cells. Functionally, cell counting kit-8 (CCK-8) and flow cytometry analyses revealed that knockdown of circPTK2 suppressed LSCC cell viability and the cell cycle while promoting cell apoptosis. Notably, silencing circPTK2 inhibited tumor growth in vivo. Mechanistically, circPTK2 functioned as a molecular sponge of miR-1278 to upregulate YAP1 expression in LSCC cells. Moreover, YAP1 knockdown inhibited malignant phenotypes of LSCC cells. The rescue experiments showed that YAP1 overexpression reversed the effects of circPTK2 on LSCC cells. Therefore, we concluded that circPTK2 facilitates LSCC progression through the miR-1278/YAP1 axis.

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Circular RNA 103862 Promotes Proliferation and Invasion of Laryngeal Squamous Cell Carcinoma Cells Through the miR-493-5p/GOLM1 Axis

Cited by 26 publications

References 32 publications

The circ_0032822 Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma Cells Through miR-141/EF3 Signaling Axis

The circ_0032822 Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma Cells Through miR-141/EF3 Signaling Axis

Hsa_circ_0042823 accelerates cancer progression via miR-877-5p/FOXM1 axis in laryngeal squamous cell carcinoma

CircPTK2 (hsa_circ_0003221) Contributes to Laryngeal Squamous Cell Carcinoma by the miR-1278/YAP1 Axis

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