CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9

Sun, Yanpeng; Zhou, Yingjie; Shi, Xiangqin; Ma, Xiaoran; Peng, Xiaodong; Xie, Yong; Cao, Xiangyang

doi:10.1080/15384101.2021.1962634

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…The regulation of cancer progression by circRNAs is often reflected in the biological behavior regulation of tumor cell proliferation, migration, invasion, and EMT. 32 As for circ_ZFR, previous studies showed that it decelerated gastric cancer progression via miR-130a/PTEN axis and miR-107/ PTEN axis, 31 and repressed cancer malignancy by miR-532-3p/FOXO4 axis. 33 Circ_ZFR aggravated PTC growth and invasion via miR-1261/C80rf4 axis, 11 facilitated the development of hepatocellular carcinoma via miR-3619-5p/CTNNB1 axis and Wnt/β-catenin pathway, 34 promoted non-small-cell lung carcinoma progression via miR-101-3p/CUL4B axis.…”

Section: Discussionmentioning

confidence: 99%

“…The regulation of cancer progression by circRNAs is often reflected in the biological behavior regulation of tumor cell proliferation, migration, invasion, and EMT 32 . As for circ_ZFR, previous studies showed that it decelerated gastric cancer progression via miR‐130a/PTEN axis and miR‐107/PTEN axis, 31 and repressed colorectal cancer malignancy by miR‐532‐3p/FOXO4 axis 33 .…”

Section: Discussionmentioning

confidence: 99%

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Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

et al. 2022

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Background Breast cancer (BC) is a common malignancy in women. Circular RNAs (circRNAs) have been reported to play a key role in the development of BC; however, the effect of circular RNA zinc finger RNA binding protein (circ_ZFR) in BC is unknown. Methods Abundances of circ_ZFR, fatty acid binding protein 7 (FABP7), and microRNA‐223‐3p (miR‐223‐3p) were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). The circular structure of circ_ZFR was validated by RNase R treatment. Cell proliferation, migration, invasion, and apoptosis were assessed by colony formation, cell counting kit‐8, Transwell, flow cytometry assays, respectively. All protein levels were determined by Western blot. Dual‐luciferase reporter assay was used to confirm the relationship between miR‐223‐3p and circ_ZFR or FABP7. A xenograft model was established to understand the effect of circ_ZFR on BC cell growth in vivo. Results The expression levels of circ_ZFR and FABP7 were higher in BC tissues and cell lines, whereas miR‐223‐3p expression was lower. Knockdown of circ_ZFR or FABP7 in BC cells reduced proliferation, migration, invasion, and epithelial mesenchymal transition (EMT), and induced apoptosis in vitro, whereas the opposite effects were observed in circ_ZFR‐overexpressed cells. Furthermore, circ_ZFR might act as a sponge for miR‐223‐3p to regulate FABP7 expression, thereby promoting the progression of BC cells in vitro and in vivo. Conclusion Circ_ZFR might act as a miRNA sponge for miR‐223‐3p to regulate FABP7, thereby promoting proliferation, migration, invasion, and EMT of BC cells, and inhibiting cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

et al. 2022

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“… 28 Additionally, Sun et al showed that circTYW1 contributed to functional recovery in rats after SCI and suppressed apoptosis in the lesion site as well as oxygen‐glucose deprivation (OGD)‐induced PC12 cells by activating ERK1/2 signaling through miR‐380/FGF9 axis. 29 Therefore, circRNAs are also have roles in function recovery after SCI. In this study, an increased expression of circSmox was discovered in LPS‐induced PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Circ_0000962 was found to have a neuroprotective effect by activating PI3K/Akt and blocking of NF‐κB via miR‐302b‐3p to suppress inflammatory response in vitro cell model of SCI 28 . Additionally, Sun et al showed that circTYW1 contributed to functional recovery in rats after SCI and suppressed apoptosis in the lesion site as well as oxygen‐glucose deprivation (OGD)‐induced PC12 cells by activating ERK1/2 signaling through miR‐380/FGF9 axis 29 . Therefore, circRNAs are also have roles in function recovery after SCI.…”

Section: Discussionmentioning

confidence: 99%

CircSmox knockdown alleviates PC12 cell apoptosis and inflammation in spinal cord injury by miR‐340‐5p/Smurf1 axis

Han

Mou

Jing

et al. 2023

Immunity Inflam & Disease

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Background Spinal cord injury (SCI) is a traumatic central nervous system disorder that leads to irreversible neurological dysfunction. Emerging evidence has shown that differentially expressed circular RNAs (circRNAs) after SCI is closely associated with the pathophysiological process. Herein, the potential function of circRNA spermine oxidase (circSmox) in functional recovery after SCI was investigated. Methods Differentiated PC12 cells stimulated with lipopolysaccharide (LPS) were employed as an in vitro model for neurotoxicity research. Levels of genes and proteins were detected by quantitative real‐time PCR and Western blot analysis. Cell viability and apoptosis were determined by CCK‐8 assay and flow cytometry. Western blot analysis was used to detect the protein level of apoptosis‐related markers. The levels of interleukin (IL)‐1β, IL‐6, IL‐8, and tumor necrosis factor (TNF)‐α. Dual‐luciferase reporter, RIP, and pull‐down assays were used to confirm the target relationship between miR‐340‐5p and circSmox or Smurf1 (SMAD Specific E3 Ubiquitin Protein Ligase 1). Results LPS elevated the levels of circSmox and Smurf1, but decreased the levels of miR‐340‐5p in PC12 cells in a dose‐dependent manner. Functionally, circSmox silencing alleviated LPS‐induced apoptosis and inflammation in PC12 cells in vitro. Mechanistically, circSmox directly sponged miR‐340‐5p, which targeted Smurf1. Rescue experiments showed that miR‐340‐5p inhibition attenuated the neuroprotective effect of circSmox siRNA in PC12 cells. Moreover, miR‐340‐5p suppressed LPS‐triggered neurotoxicity in PC12 cells, which was reversed by Smurf1 overexpression. Conclusion CircSmox enhances LPS‐induced apoptosis and inflammation via miR‐340‐5p/Smurf1 axis, providing an exciting view of the potential involvement of circSmox in SCI pathogenesis.

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“…Recently, a special type of circRNAs has been identified, called tRNA intronic circular RNAs, which is spliced from endogenous tRNAs under the action of RtcB ligase and the tRNA splicing endonuclease complex ( 9 ). CircRNAs have various biological functions and play vital roles in the occurrence, development, invasion, diagnosis, and prognosis of tumors ( 10 – 14 ), and are also important contributors to the development of cardiovascular ( 15 ), neurological ( 16 ), orthopedic ( 17 ), and other diseases.…”

Section: Introductionmentioning

confidence: 99%

Circular RNAs and Their Role in Exosomes

Han

Chen²,

Guo

et al. 2022

Front. Oncol.

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As a novel class of endogenous non-coding RNAs discovered in recent years, circular RNAs (circRNAs) are highly conserved and stable covalently closed ring structures with no 5′-end cap or 3′-end poly(A) tail. CircRNAs are formed by reverse splicing, mainly by means of a noose structure or intron complementary pairing. Exosomes are tiny discoid vesicles with a diameter of 40-100 nm that are secreted by cells under physiological and pathological conditions. Exosomes play an important role in cell-cell communication by carrying DNA, microRNAs, mRNAs, proteins and circRNAs. In this review, we summarize the biological functions of circRNAs and exosomes, and further reveal the potential roles of exosomal circRNAs in different diseases, providing a scientific basis for the diagnosis, treatment, and prognosis of a wide variety of diseases.

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CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9

Cited by 8 publications

References 25 publications

Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

Circ_ZFR affects FABP7 expression to regulate breast cancer progression by acting as a sponge for miR‐223‐3p

CircSmox knockdown alleviates PC12 cell apoptosis and inflammation in spinal cord injury by miR‐340‐5p/Smurf1 axis

Circular RNAs and Their Role in Exosomes

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