CircRNA_28313/miR-195a/CSF1 axis modulates osteoclast differentiation to affect OVX-induced bone absorption in mice

Abstract

Osteoblastic bone formation and osteoclastic bone resorption dynamically maintain the bone homeostasis; in the present study, we attempt to investigate the mechanism of the excessive activation of osteoclasts inducing the deregulation of bone homeostasis from the perspective of non-coding RNA regulation. Differentially expressed patterns of circRNAs were examined in non-treated and RANKL + CSF1-treated bone marrow monocyte/macrophage (BMM) cells and differentially-expressed miRNAs during osteoclast differentiation were analyzed and identified. We found that circRNA_28313 was significantly induced by RANKL + CSF1 treatment. circRNA_28313 knockdown significantly inhibited RANKL + CSF1-induced differentiation of osteoclasts within BMM cells in vitro, while suppressed ovariectomized (OVX)-induced bone resorption in mice in vivo. Via bioinformatics analyses, it has been demonstrated that miR-195a might bind to circRNA_28313 and CSF1 and together form a circRNA-miRNA-mRNA network. circRNA_28313 relieves miR-195a-mediated suppression on CSF1 via acting as a ceRNA, therefore modulating the osteoclast differentiation in BMM cells. In conclusion, circRNA_28313, miR-195a, and CSF1 form a ceRNA network to function in RANKL + CSF1-induced osteoclast differentiation, thus affecting OVX-induced bone absorption in mice.

Loading‐induced antitumor capability of murine and human urine

“…CD105 is part of the TGFβ receptor complex and involved in tumor‐related angiogenesis 55 . CSF1 is a hematopoietic growth factor and is linked to bone homeostasis and progression of many cancers including breast cancer 30 …”

“…In searching for the potential mechanism of differentiating unconditioned urine medium (UM) from loading‐conditioned UM, we examined the role of Lrp5 and Lrp6, co‐receptors of Wnt signaling 29 . We also evaluated the role of macrophage colony‐stimulating factor 1 (CSF1), which is linked to bone homeostasis and the progression of many cancers 30 …”

Identification and analysis of long non-coding RNAs that are involved in inflammatory process in response to transmissible gastroenteritis virus infection

“…The lncRNAs play roles in regulating transcription, translation, and protein translocation [21][22][23][24][25]. LncRNAs can regulate translation by interacting with miRNA or act as precursors of miRNA [26][27][28].…”

Long non‐coding RNA expressed in macrophage co‐varies with the inflammatory phenotype during macrophage development and polarization

“…However, the effects of circRNAs in macrophage differentiation and polarization have not to be widely explored. Whether circRNA involved in the activation of macrophages and whether it affect the development of macrophages by regulating lncRNAs require more further experiments to detect . Unfortunately, there is a defect in our paper that many studies on the mechanisms of lncRNAs expressed in macrophages have not been studied intensively.…”

“…Whether circRNA involved in the activation of macrophages and whether it affect the development of macrophages by regulating lncRNAs require more further experiments to detect. 126 Unfortunately, there is a defect in our paper that many studies on the mechanisms of lncRNAs expressed in macrophages have not been studied intensively. It is expected that more exploration about lncRNAs in regulating innate immune response and macrophage-associated biological processes will be carried out.…”