CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway

Abstract

Reportedly, circular RNAs (circRNAs) are crucial regulators in cancer progression. Nonetheless, the molecular mechanism of circRNAs in hepatocellular carcinoma (HCC) has not been fully clarified. Gene expression omnibus (GEO) database was employed to screen out the differentially expressed circRNAs in HCC. qRT-PCR and western blot were executed to detect circ_0001806 expression, miR-193a-5p expression, and MMP16 mRNA and protein expressions in HCC. The effect of circ_0001806 on HCC was analyzed by the CCK-8 method and Transwell experiment. RIP assay, pull-down experiment, and dual-luciferase reporter gene experiment were applied to validate the targeting relationships among circ_0001806, miR-193a-5p, and MMP16. Circ_0001806 was up-modulated in HCC tissues and cell lines. Knockdown of circ_0001806 impeded the multiplication, migration, and invasion of HCC cells. Circ_0001806 could up-regulate MMP16 expression through repressing miR-193a-5p, thereby facilitating the malignant biological behaviors of HCC. Circ_0001806 promoted HCC progression by regulating miR-193a-5p/MMP16 axis.

Whole blood circular RNA hsa_circ_0002171 serves as a potential diagnostic biomarker for human adenovirus pneumonia in children

“…circRNAs exert ubiquitous biological functions in various tissues and cells ( 13 , 14 , 28 ). They have recently been reported as a unique class of long noncoding RNAs that affect the expression of a target miRNA and thereby regulate mRNA expression and influence many biological processes ( 29 , 30 ).…”

Hsa_circ_0000285 knockdown inhibits the progression of hepatocellular carcinoma by sponging miR-582-3p to regulate CCNB2 expression

“…The cells and tissue specimens were subjected to RIPA Lysis Buffer (Beyotime) treatment for the isolation of total proteins. 29 The protein concentrations were assessed with the aid of a BCA Protein Assay Kit (Beyotime). Electrophoresis of the total protein was accomplished on an SDS-PAGE gel before the separated proteins were transplanted onto PVDF membranes.…”

Four differentially expressed exosomal miRNAs as prognostic biomarkers and therapy targets in endometrial cancer: Bioinformatic analysis

“…As to hsa-miR-193a-5p in other cancers, there have been a lots of special research reports, like, on ovarian cancer, [75,76] on lung cancer, [77,78] on breast cancer [79][80][81][82] on melanoma, [83] on hepatoblastoma, [84] on esophageal squamous cell carcinoma, [85] on gastric cancer, [86,87] on rhabdomyosarcoma, [88] on papillary thyroid carcinoma, [89] on colorectal cancer, [90][91][92][93][94][95] on cervical cancer, [96,97] on nasopharyngeal carcinoma, [98] on acute myeloid leukemia, [99] on clear-cell renal cell carcinoma, [100] on pancreatic cancer, [101,102] on glioma, [103] on pediatric chordomas, [104] on bladder cancers, [105] on osteosarcoma, [106,107] on prostate cancer, [108][109][110] and on HCC. [111][112][113] Among those special research reports on non-EC cancers, most showed that the effects of hsa-miR-193a-5p on the relative cancers were negatively oncogenic, as hsa-miR-193a-5p was a potential tumor suppressor. [75][76][77][78]…”