CircRNA expression profiles in deltamethrin-susceptible and -resistant Culex pipiens pallens (Diptera: Culicidae)

Lv, Yuan; Li, Xixi; Zou, Feifei; Shen, Bo

doi:10.1016/j.cbpb.2022.110750

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…Elevated transcript levels of CYP6a23 as well as increased levels of CYP4g15, albeit not significantly, were measured in L. decemlineata exposed to a lower dose of spinosad. Elevated expression levels of the latter were notably reported in a strain of the mosquito Culex pipiens pallens that displayed resistance to the pyrethroid deltamethrin (Lv et al, 2022). Transcript levels of CYP6BJ1, also known as CYP6a23, were significantly increased in L. decemlineata larvae exposed to cyhalothrin (Wan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, Leptinotarsa decemlineata

Bastarache

Bouafoura

Omakele

et al. 2022

Arch Insect Biochem Physiol

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Section: Discussionmentioning

confidence: 99%

Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, Leptinotarsa decemlineata

Bastarache

Bouafoura

Omakele

et al. 2022

Arch Insect Biochem Physiol

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“…Although a class of endogenous ncRNAs, including miRNAs [ 51 , 58 ], piRNAs [ 59 – 60 ] and lncRNAs [ 61 – 62 ], which play crucial roles in various biological processes, including development, oogenesis, metabolism and interactions between pathogens and vectors, have been well characterized in mosquito species, the function of circRNAs remains unclear in mosquitoes. To date, only the circRNA expression profiles in Culex pipiens pallens have been outlined, which suggests that an overexpressed circRNA in deltamethrin-resistant strains might participate in deltamethrin resistance through the cpp-miR-1671/ CYP4G15 pathway [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…The genome-wide identification of insect circRNAs was first performed in Drosophila melanogaster in 2014 [ 17 ]. Subsequently, a relatively comprehensive identification of circRNAs was performed in Bombyx mori [ 18 ], Apis mellifera [ 19 ], Dendrolimus punctatus [ 20 ], Helicoverpa armigera [ 21 ] and Culex pipiens pallens [ 22 ]. Nevertheless, only a few circRNAs have been functionally characterized.…”

Section: Introductionmentioning

confidence: 99%

Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis

Gao

Chen

et al. 2023

PLoS Pathog

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Aedes albopictus shows a rapid global expansion and dramatic vectorial capacity for various arboviruses, thus posing a severe threat to global health. Although many noncoding RNAs have been confirmed to play functional roles in various biological processes in Ae. albopictus, the roles of circRNA remain a mystery. In the present study, we first performed high-throughput circRNA sequencing in Ae. albopictus. Then, we identified a cysteine desulfurase (CsdA) superfamily gene-originated circRNA, named aal-circRNA-407, which was the third most abundant circRNA in adult females and displayed a fat body highly expressed manifestation and blood feeding-dependent onset. SiRNA-mediated knockdown of circRNA-407 resulted in a decrease in the number of developing follicles and a reduction in follicle size post blood meal. Furthermore, we demonstrated that circRNA-407 can act as a sponge of aal-miR-9a-5p to promote the expression of its target gene Foxl and eventually regulate ovarian development. Our study is the first to report a functional circRNA in mosquitoes, expanding our current understanding of important biological roles in mosquitoes and providing an alternative genetic strategy for mosquito control.

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“…However, research on insect circRNAs is currently still in the preliminary stage. There has been limited documentation for a small number of insects including Culex pipiens pallens [12], Apis mellifera [13], Bombyx mori [14], Drosophila [15], and Laodelphax striatellus [16]. Accumulating evidence has demonstrated that circRNAs are engaged in the regulation of interactions between insects and pathogens/parasites [12,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…There has been limited documentation for a small number of insects including Culex pipiens pallens [12], Apis mellifera [13], Bombyx mori [14], Drosophila [15], and Laodelphax striatellus [16]. Accumulating evidence has demonstrated that circRNAs are engaged in the regulation of interactions between insects and pathogens/parasites [12,[16][17][18]. For example, based on high-throughput sequencing of the midgut tissues of both uninfected and BmCPV-infected B. mori, Hu et al [17] detected that 400 host circRNAs were significantly differentially expressed following BmCPV infection.…”

Section: Introductionmentioning

confidence: 99%

CircRNAs and their regulatory networks associated with the antioxidant enzymes and immune responses of Asian honey bee larvae to fungal infection

Guo

Zhang

Gao

et al. 2023

Preprint

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Non–coding RNA (ncRNA) plays an important role in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic investigation, and molecular verification of circRNAs in Apis cerana cerana larval guts were conducted, and the expression pattern of larval circRNAs during Ascosphaera apis infection was analyzed. This was followed by exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs in the larval guts of A. c. cerana were identified, with a length distribution ranging from 15 nt to 96007 nt. Additionally, 45, 33, and 48 up-regulated circRNAs, as well as 110, 62, and 38 down-regulated circRNAs were identified in the A. apis–inoculated 4–, 5–, and 6–day–old larval guts in comparison with the corresponding uninoculated larval guts. These DEcircRNAs were predicted to target 29, 25, and 18 parental genes, which were relative to 12, 20, and 17 GO terms as well as 144, 114, and 61 KEGG pathways, including five cellular and four humoral immune pathways containing melanization, phagosomes, lysosomes, endocytosis, apoptosis, MAPK, Ras, and Jak–STAT signaling pathways. Furthermore, complex competing endogenous RNA (ceRNA) regulatory networks were detected as being formed among DEcircRNAs, DEmiRNAs, and DEmRNAs. The target DEmRNAs were engaged in 36, 47, and 47 GO terms as well as 331, 332, and 331 pathways, including six cellular and six humoral immune-related pathways. In total, nineteen DEcircRNAs, five DEmiRNAs, and three mRNAs were included in the sub-networks relative to three antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). Finally, back–splicing sites within 15 circRNAs and the difference in the 15 DEcircRNAs expression between uninoculated and A.apis–inoculated larval guts were confirmed utilizing molecular methods. These findings not only enrich our understanding of bee host–fungal pathogen interactions, but also lay a foundation for illuminating the mechanism underlying the DEcircRNA–mediated immune defense of A. c. cerana larvae against A. apis invasion.

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CircRNA expression profiles in deltamethrin-susceptible and -resistant Culex pipiens pallens (Diptera: Culicidae)

Cited by 8 publications

References 41 publications

Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, Leptinotarsa decemlineata

Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, Leptinotarsa decemlineata

Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis

CircRNAs and their regulatory networks associated with the antioxidant enzymes and immune responses of Asian honey bee larvae to fungal infection

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