Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, <i>Leptinotarsa decemlineata</i>

Bastarache, Pierre; Bouafoura, Raed; Omakele, Enock; Moffat, Chandra E.; Vickruck, Jess; Morin, Pier Jr

doi:10.1002/arch.21993

Cited by 4 publications

(11 citation statements)

References 49 publications

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“…Higher expression levels are observed in the 1st instar and gradually decrease in the later instars. A homolog of this gene was highly upregulated in Colorado potato beetle, Leptinotarsa decemlineata , in response to spinosyn applied directly to the abdomen of adults 58 . Using predictive bioinformatic software, C2, C36 and C51 on spinosyn A and C2, C27 and C52 on spinosyn D were consistently identified as potential targets of oxidation by cytochrome P 450 s, suggesting that these cytochrome P 450 enzymes may be involved in spinosyn detoxification.…”

Section: Resultsmentioning

confidence: 99%

“…Carboxylesterases are correlated with spinosyn resistance in a number of arthropod species, such as Frankliniella occidentalis (insecticide: Success2, Dow Agrosciences), P. xylostella (insecticide from Dow Agrosciences) and Musca domestica (insecticide from Dow Agrosciences) 59–61 . As well, venom carboxylesterase 6‐like was upregulated in spinosyn‐treated adults of the Colorado potato beetle 58 …”

Section: Resultsmentioning

confidence: 99%

“…Spinosyn exposure has also been observed to cause hormonal abnormalities. 58,73,74 In our study, an increase in genes that encode juvenile hormone esterase (JHE) (LOC1113498447, LOC113492812, LOC113504904, LOC113505877, LOC113505925, LOC113505295, LOC113492819), an enzyme involved in JH degradation and, thus, in regulating insect development from embryogenesis to metamorphosis, [75][76][77][78] is observed in response to egg treatment by HS (Supporting Information, Table S2). LOC11349847 expression is increased in both 1st and 3rd instar caterpillars that emerged from eggs treated with HS (Fig.…”

Section: Effects Of Sublethal Concentration Of Spinosyn (Spinosyn a A...mentioning

confidence: 99%

“…[59][60][61] As well, venom carboxylesterase 6-like was upregulated in spinosyn-treated adults of the Colorado potato beetle. 58 Through the addition of glutathione to an electrophile, GSTs either decrease the activity of the compound or facilitate their excretion as well as sequestration. 62 Though GSTs may be involved in spinosyn detoxification, 56 these enzymes do not appear to play a major role.…”

Section: Effects Of Sublethal Concentration Of Spinosyn (Spinosyn a A...mentioning

confidence: 99%

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Effect of sublethal concentrations of the bioinsecticide spinosyn treatment of Trichoplusia ni eggs on the caterpillar and its parasitoid, Trichogramma brassicae

Smith,

Chen,

Lafleur

et al. 2024

Pest Management Science

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BACKGROUNDIntegrated Pest Management (IPM) seeks to combine multiple management strategies for optimal pest control. One method that is successfully employed in IPM is the use of beneficial organisms. However, in severe circumstances when pest insects exceed threshold limits, insecticides may still need to be implemented. Thus, understanding the effects of insecticides on biocontrol agents, such as parasitoid wasps, is paramount to ensure sustainable agroecosystems. Sublethal effects of the bioinsecticide spinosyn, a mixture of the bacterial Saccharopolyspora spinosa (Mertz and Yao) fermentation products spinosyn A and D, on eggs of Trichoplusia ni (Hübner), a cruciferous crop pest, and its egg parasitoid Trichogramma brassicae (Bezdenko) was investigated.RESULTSThe LC50 for spinosyn A and D (dissolved in ethanol) on T. ni eggs is 54 ng/mL. Transcriptomics on caterpillars (1st and 3rd instars) that hatched from eggs treated with sublethal concentrations of spinosyn identified the upregulation of several genes encoding proteins that may be involved in insecticide resistance including detoxification enzymes, such as cytochrome P450s, glutathione S‐transferases and esterases. Sublethal T. ni egg treatments did not affect parasitoid emergence, however, there was a marked increase in the size of T. brassicae hind tibia and wings that emerged from spinosyn‐treated eggs.CONCLUSIONSFor the caterpillar, treatment of eggs with sublethal concentrations of spinosyn may induce insecticide resistance mechanisms. For the parasitoids, their increased size when reared in spinosyn‐treated eggs suggests that the emerged wasps may have a higher performance.This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Effects Of Sublethal Concentration Of Spinosyn (Spinosyn a A...mentioning

confidence: 99%

Section: Effects Of Sublethal Concentration Of Spinosyn (Spinosyn a A...mentioning

confidence: 99%

See 2 more Smart Citations

Effect of sublethal concentrations of the bioinsecticide spinosyn treatment of Trichoplusia ni eggs on the caterpillar and its parasitoid, Trichogramma brassicae

Smith,

Chen,

Lafleur

et al. 2024

Pest Management Science

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“…In addition to the effect of exogenous substances on insect reproduction and development, the detoxification and metabolism of exogenous substances by pests have been extensively studied [ 28 , 29 , 30 , 31 ]. In this study, 28 DEGs were primarily enriched in the “drug metabolism- other enzymes”, “glutathione metabolism” and “Metabolism of xenobiotics by cytochrome P450” pathways.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide

Zhou

Qiu

et al. 2023

IJMS

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Chilo suppressalis is one of the most damaging rice pests in China’s rice-growing regions. Chemical pesticides are the primary method for pest control; the excessive use of insecticides has resulted in pesticide resistance. C. suppressalis is highly susceptible to cyproflanilide, a novel pesticide with high efficacy. However, the acute toxicity and detoxification mechanisms remain unclear. We carried out a bioassay experiment with C. suppressalis larvae and found that the LD10, LD30 and LD50 of cyproflanilide for 3rd instar larvae was 1.7 ng/per larvae, 6.62 ng/per larvae and 16.92 ng/per larvae, respectively. Moreover, our field trial results showed that cyproflanilide had a 91.24% control efficiency against C. suppressalis. We investigated the effect of cyproflanilide (LD30) treatment on the transcriptome profiles of C. suppressalis larvae and found that 483 genes were up-regulated and 305 genes were down-regulated in response to cyproflanilide exposure, with significantly higher CYP4G90 and CYP4AU10 expression in the treatment group. The RNA interference knockdown of CYP4G90 and CYP4AU10 increased mortality by 20% and 18%, respectively, compared to the control. Our results indicate that cyproflanilide has effective insecticidal toxicological activity, and that the CYP4G90 and CYP4AU10 genes are involved in detoxification metabolism. These findings provide an insight into the toxicological basis of cyproflanilide and the means to develop efficient resistance management tools for C. suppressalis.

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Role of CYP9E2 and a long non-coding RNA gene in resistance to a spinosad insecticide in the Colorado potato beetle, Leptinotarsa decemlineata

Kaplanoglu,

Scott,

Vickruck

et al. 2024

PLoS ONE

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Spinosads are insecticides used to control insect pests, especially in organic farming where limited tools for pest management exist. However, resistance has developed to spinosads in economically important pests, including Colorado potato beetle (CPB), Leptinotarsa decemlineata. In this study, we used bioassays to determine spinosad sensitivity of two field populations of CPB, one from an organic farm exposed exclusively to spinosad and one from a conventional farm exposed to a variety of insecticides, and a reference insecticide naïve population. We found the field populations exhibited significant levels of resistance compared with the sensitive population. Then, we compared transcriptome profiles between the two field populations to identify genes associated primarily with spinosad resistance and found a cytochrome P450, CYP9E2, and a long non-coding RNA gene, lncRNA-2, were upregulated in the exclusively spinosad-exposed population. Knock-down of these two genes simultaneously in beetles of the spinosad-exposed population using RNA interference (RNAi) resulted in a significant increase in mortality when gene knock-down was followed by spinosad exposure, whereas single knock-downs of each gene produced smaller effects. In addition, knock-down of the lncRNA-2 gene individually resulted in significant reduction in CYP9E2 transcripts. Finally, in silico analysis using an RNA-RNA interaction tool revealed that CYP9E2 mRNA contains multiple binding sites for the lncRNA-2 transcript. Our results imply that CYP9E2 and lncRNA-2 jointly contribute to spinosad resistance in CPB, and lncRNA-2 is involved in regulation of CYP9E2 expression. These results provide evidence that metabolic resistance, driven by overexpression of CYP and lncRNA genes, contributes to spinosad resistance in CPB.

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Spinosad‐associated modulation of select cytochrome P450s and glutathione S‐transferases in the Colorado potato beetle, Leptinotarsa decemlineata

Abstract: The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an insect pest that threatens potato crops. Multiple options exist to limit the impact of this pest even though insecticides remain a primary option for its control.

Cited by 4 publications

References 49 publications

Effect of sublethal concentrations of the bioinsecticide spinosyn treatment of Trichoplusia ni eggs on the caterpillar and its parasitoid, Trichogramma brassicae

Effect of sublethal concentrations of the bioinsecticide spinosyn treatment of Trichoplusia ni eggs on the caterpillar and its parasitoid, Trichogramma brassicae

Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide

Role of CYP9E2 and a long non-coding RNA gene in resistance to a spinosad insecticide in the Colorado potato beetle, Leptinotarsa decemlineata

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