CircRNA circ_0026344 as a prognostic biomarker suppresses colorectal cancer progression via microRNA-21 and microRNA-31

Yuan, Yuan; Liu, Wei; Zhang, Yangmei; Zhang, Youwei; Sun, Shuai

doi:10.1016/j.bbrc.2018.06.089

Cited by 104 publications

(83 citation statements)

References 19 publications

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“…In the current study, miR-183, a recognized oncogene [24], was found to be a downstream gene of circ_0026344 since it was negatively regulated by circ_0026344. This result was analogous with a preceding study, in which miR-21 and miR-31 were revealed as two downstream genes of circ_0026344 [13]. Another study implied that miR-183 facilitated proliferation and suppressed apoptosis in CRC via targeting ABCA1 [24].…”

Section: Discussionsupporting

confidence: 89%

“…Also, the role of circRNAs in various kinds of cancers has been studied. A previous study demonstrated circ_0026344 as a tumour suppressive gene in CRC progression, as overexpression of circ_0026344 suppressed CRC cells growth and invasion while promoted apoptosis [13]. However, great effort is still required to fully appreciate the complexity of circ_0026344.…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, certain circRNAs have been considered as promising biomarkers and therapeutic targets since they are aberrantly expressed in tumours and involved in the onset and progression of tumours [11,12]. A previous study has revealed that circ_0026344 was downregulated in CRC tissues and circ_0026344 overexpression led to CRC cell lose both in vitro and in vivo [13], indicating circ_0026344 worked as a tumour-suppressive gene. The present work attempted to study whether circ_0026344 also play a role in CRC metastasis induced by CXCL8 combined with CCL20.…”

Section: Introductionmentioning

confidence: 99%

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Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Shen

Cheng

Liu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: CircRNA circ_0026344 was previously revealed as a tumour-suppressive gene in colorectal cancer (CRC) progression. The purpose of this research was to investigate the role of circ_0026344 in CRC cells metastasis induced by chemokines. Methods: Two human CRC cell lines SW480 and Caco-2 were treated by CCL20 and CXCL8. Cell proliferation, migration/invasion, expression of epithelial-mesenchymal transition (EMT) inducers and the expression of circ_0026344 were measured using sulforhodamine B assay, Transwell chamber, western blot and qRT-PCR, respectively. The effects of circ_0026344 on CRC cells migration/invasion and the expression of EMT inducers were evaluated. Moreover, the downstream miRNA and signalling pathways of circ_0026344 were studied. Results: CCL20 and CXCL8 synergized to facilitate the proliferation, migration and invasion of CRC cells. At the meantime, E-cadherin was downregulated, whereas N-cadherin, Vimentin and Snail were upregulated by CCL20 and CXCL8 co-stimulation, which was accompanied by the mobilization of PI3K/ AKT/ERK signalling. More interestingly, the expression of circ_0026344 was down-regulated by CCL20 and CXCL8 co-stimulation. Silence of circ_0026344 increased the migratory and invasive capacities of CRC cells and increased EMT process as well. Overexpression of circ_0026344 led to a contrary impact. miR-183 was negatively regulated by circ_0026344, and the inhibitory effects of circ_0026344 overexpression on Wnt/b-catenin pathway were reversed when miR-183 was overexpressed. Conclusion: Overexpression of circ_0026344 restrained CRC metastasis and EMT induced by CCL20 and CXCL8 synergistical treatment. miR-183 was a downstream effector of circ_0026344, and the antitumour function of circ_0026344 might be involved in the repressed Wnt/b-catenin signalling. HIGHLIGHTS 1. CCL20 and CXCL8 synergize to decrease the expression of circ_0026344; 2. Silence of circ_0026344 promotes CRC cells migration, invasion and EMT process; 3. miR-183 is a downstream effector of circ_0026344.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Shen

Cheng

Liu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…CircRNAs can regulate transcription or splicing, and interact with RNA binding proteins (RBPs) [13,14]. Remarkably, emerging evidence indicates that some circRNAs can serve as competitive endogenous RNA (ceRNA) of microRNA (miRNA) through its miRNA response elements (MREs), which can competitively binding miRNAs to release or attenuate translational repression or degradation through sequestering miRNAs away from parental mRNA [15,16]. Moreover, circRNAs have been reported to play essential roles in many life processes, such as aging, insulin secretion, tissue development, atherosclerotic vascular disease risk, cardiac hypertrophy, and cancer [17].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis

Qiao¹,

Ding²,

Yan³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) are evolutionary conserved circular non-coding RNAs that play a role in several diseases by sequestering (sponging) microRNAs (miRNAs). However, their role in psoriasis remains unclear. In the present study, we investigated the expression of circRNAs and analyzed their potential functions in psoriasis. Methods: The SBC human ceRNA array V1.0 was used to analyze circRNA expression in psoriatic lesions and normal healthy skin tissues. Functional analyses were performed using Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Putative miRNA response elements (MREs) were identified using miRNA target prediction software. Six upregulated circRNAs were verified by quantitative real-time reverse transcription polymerase chain reaction in psoriatic lesions and healthy skin tissues. Results: A total of 4956 circRNAs (3016 upregulated and 1940 downregulated; fold change ≥2 and p< 0.05) were identified as differentially expressed in psoriasis. Furthermore, 4405 MREs were identified among the differentially expressed circRNAs. hsa_circ_0061012 was upregulated in psoriatic lesions compared with normal healthy skin tissues. The top five MREs of hsa_circ_0061012 were hsa-miR-7157-5p, hsa-miR-4769-3p, hsa-miR-6817-5p, hsa-miR-4310, and hsa-miR-6882-3p. GO analysis was carried out to investigate the biological functions enriched among the upregulated targets of five miRNAs in psoriasis. The GO analysis identified that most of top 30 of GO enrichment are related to psoriasis. Conclusion: hsa_circ_0061012 might be a candidate biomarker for psoriasis. The results provide a new perspective for a better understanding of ceRNA-mediated gene regulation in psoriasis, and provide a novel theoretical basis for further studies on the function of circRNA in psoriasis.

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“…Furthermore, through heightening the level of ITCH and restraining the Wnt/β-catenin pathway, cir-ITCH suppressed the proliferation of CRC cells [53]. In addition, circ_0026344 reduced the cell invasion and growth of CRC while accelerating apoptosis through sponging miR-31 and miR-21 in CRC [54]. Moreover, hsa_circ_0000567 knockdown promoted CRC cell migration and proliferation in vitro [55].…”

Section: Circrnas That Act As Tumour Suppressors In Crcmentioning

confidence: 98%

The Regulatory Functions of Circular RNAs in Digestive System Cancers

Yuan

Yuán

et al. 2020

Cancers

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Circular ribonucleic acids (circRNAs), which are a type of covalently closed circular RNA, are receiving increasing attention. An increasing amount of evidence suggests that circRNAs are involved in the biogenesis and development of multiple diseases such as digestive system cancers. Dysregulated circRNAs have been found to act as oncogenes or tumour suppressors in digestive system cancers. Moreover, circRNAs are related to ageing and a wide variety of processes in tumour cells, such as cell apoptosis, invasion, migration, and proliferation. Moreover, circRNAs can perform a remarkable multitude of biological functions, such as regulating splicing or transcription, binding RNA-binding proteins to enable function, acting as microRNA (miRNA) sponges, and undergoing translated into proteins. However, in digestive system cancers, circRNAs function mainly as miRNA sponges. Herein, we summarise the latest research progress on biological functions of circRNAs in digestive system cancers. This review serves as a synopsis of potential therapeutic targets and biological markers for digestive system cancer.

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CircRNA circ_0026344 as a prognostic biomarker suppresses colorectal cancer progression via microRNA-21 and microRNA-31

Cited by 104 publications

References 19 publications

Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183

Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis

The Regulatory Functions of Circular RNAs in Digestive System Cancers

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