Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis

Qiao, Meng; Ding, Jian; Yan, Jianjun; Li, Ronghua; Jiao, Jian; Sun, Qing

doi:10.1159/000493952

Cited by 59 publications

(44 citation statements)

References 32 publications

(36 reference statements)

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“…Circular RNAs (circRNAs) are a new class of noncoding RNA molecules that extensively exist in the cytoplasm of eukaryotic cells . It is known that circRNAs are different from linear RNAs, lacking 5′ to 3′ polarity and polyadenylated tail and having great stability . CircRNAs have a variety of functions, such as regulating splicing and transcription, binding proteins, and transporting RNAs .…”

Section: Introductionmentioning

confidence: 99%

Dysregulated circRNA_100876 suppresses proliferation of osteosarcoma cancer cells by targeting microRNA‐136

Jin

Chen

Qiu

et al. 2019

J of Cellular Biochemistry

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The aim of this study was to explore the regulatory mechanism of circRNA_100876/ microRNA‐136 (miR‐136) axis in the development and progression of osteosarcoma cancer. Quantitative real‐time polymerase chain reaction (RT‐qPCR) was used to evaluate the expression levels of circRNA_100876 and miR‐136 in osteosarcoma cancer samples and the adjacent nontumor tissues. Then, cell proliferation, cell cycle, apoptosis, and migration of circRNA_100876‐knocked down cells were analyzed by in vitro and in vivo experiments, using cell counting kit‐8 (CCK‐8), flow cytometry, and transwell and tumorigenesis assays. The expression of circRNA_100876 was found to be significantly upregulated in osteosarcoma, and was closely correlated with the tumor size and tumor differentiation degree. In addition, the knockdown of circRNA_100876 could significantly inhibit the tumor growth, both in vitro and in vivo. Flow cytometry and Western blot analysis results showed that the downregulation of circRNA_100876 inhibited osteosarcoma cells proliferation via promoting apoptosis and arresting more cells in the G2/M stage, as suggested by the expression of apoptosis and cell cycle pathway‐related proteins, which changed consistently. Furthermore, the level of miR‐136 was negatively correlated with the expression of circRNA_100876, and miR‐136 inhibitors were able to reverse the suppression of cell proliferation induced by silencing circRNA_100876. Our study demonstrates that the dysregulation of circRNA_100876 could induce apoptosis and arrest the cell cycle at G2/M stage, followed by suppression of cell proliferation in osteosarcoma, while silencing miR‐136 could restore the cell growth. Therefore, circRNA_100876 might serve as a promising biomarker and treatment target for osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

Dysregulated circRNA_100876 suppresses proliferation of osteosarcoma cancer cells by targeting microRNA‐136

Jin

Chen

Qiu

et al. 2019

J of Cellular Biochemistry

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“…The authors found almost 5,000 differentially expressed circRNAs of which the majority was upregulated in psoriasis. Six differentially expressed circRNAs were then selected for verification by RT-qPCR, but only one of these, hsa_circ_0061012, could be verified [46]. Again, we did not detect this circRNA and could not examine if it is differentially expressed in our samples.…”

Section: Discussionmentioning

confidence: 98%

“…Another recent study profiled circRNA expression in three psoriatic lesions and three normal healthy skin tissues [46]. The RNA from these samples was amplified prior to microarray analysis.…”

Section: Discussionmentioning

confidence: 99%

High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome

Moldovan

Okholm

Andersen

et al. 2019

Preprint

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Background: Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation and abnormal differentiation of keratinocytes. It is one of the most prevalent chronic inflammatory skin condition in adults worldwide, with a considerable negative impact on quality of life. Circular RNAs (circRNAs) are a recently identified type of non-coding RNA with diverse cellular functions related to their exceptional stability. In particular, some circRNAs can bind and regulate microRNAs (miRNAs), a group of RNAs that play a role in the pathogenesis of psoriasis. The aim of this study was to characterize the circRNAome in psoriasis and to assess potential correlations to miRNA expression patterns. Results:Using high-throughput RNA-sequencing (RNA-seq) and NanoString nCounter technology, we found a substantial down-regulation of circRNA expression in lesional skin compared to non-lesional skin from psoriasis patients. We saw that this mainly applies to the epidermis by analyzing laser capture microdissected tissues and by RNA chromogenic in situ hybridization (CISH). We also found that the majority of the circRNAs were downregulated independent of their corresponding linear host genes. The observed downregulation of circRNAs in psoriasis was not due to altered expression levels of factors known to affect circRNA biogenesis, nor because lesional skin contained an increased number of inflammatory cells such as lymphocytes. Finally, we saw that the overall differences in available miRNA binding sites on the circRNAs between lesional and non-lesional skin did not correlate with differences in miRNA expression patterns. Conclusions:We have performed the first genome-wide circRNA profiling of paired lesional and non-lesional skin from psoriasis patients and revealed that circRNAs are much less abundant in the lesional samples. Whether this is a cause or a consequence of the disease remains to be revealed, 3 however, we found no evidence that the loss of miRNA binding sites on the circRNAs could explain differences in miRNA expression reported between lesional and non-lesional skin. BackgroundPsoriasis is one of the most common chronic inflammatory skin conditions, with 1-3% of the adult population affected worldwide [1]. It is defined by a pronounced hyperproliferation and deficient terminal differentiation of the keratinocytes. Moreover, a complex interplay between different cell types (e.g. T cells and dendritic cells) and a variety of cytokines are known to contribute in the development of psoriasis. The pathogenesis is also based on a complex synergy between genetic predisposition, major histocompatibility alleles, and a variety of environmental triggers [2]. From the molecular point of view, however, the mechanisms responsible for the interactions between keratinocytes and inflammatory cells infiltrating the epidermis are still not fully understood. The analysis of the molecular backdrop of psoriasis has described numerous disease-associated genes and proteins with abnormal expression patterns [3], but little is...

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“…It is suggested that hsa‐miR‐6817‐5p was closely associated with hsa_circ_0061012, which was up‐regulated in psoriasis significantly. Psoriasis was related well to the regulation of NF‐kappaB, whose activity could be weakened by NELL‐1 …”

Section: Discussionmentioning

confidence: 99%

The roles of circRFWD2 and circINO80 during NELL‐1‐induced osteogenesis

Huang

Cen

Zhang

et al. 2019

J Cellular Molecular Medi

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Bone defects caused heavy social and economic burdens worldwide. Nel‐like molecule, type 1 (NELL‐1) could enhance the osteogenesis and the repairment of bone defects, while the specific mechanism remains to be elucidated. Circular RNAs (circRNAs) have been found to play critical roles in the tissue development and serve as biomarkers for various diseases. However, it remains unclear that the expression patterns of circRNAs and the roles of them played in recombinant NELL‐1‐induced osteogenesis of human adipose‐derived stem cells (hASCs). In this study, we performed RNA‐sequencing to investigate the expression profiles of circRNAs in recombinant NELL‐1‐induced osteogenic differentiation and identified two key circRNAs, namely circRFWD2 and circINO80. These two circRNAs were confirmed to be up‐regulated during recombinant NELL‐1‐induced osteogenesis, and knockdown of them affected the positive effect of NELL‐1 on osteogenesis. CircRFWD2 and circINO80 could interact with hsa‐miR‐6817‐5p, which could inhibit the osteogenesis. Silencing hsa‐miR‐6817‐5p could partially reverse the negative effect of si‐circRFWD2 and si‐circINO80 on the osteogenesis. Therefore, circRFWD2 and circINO80 could regulate the expression of hsa‐miR‐6817‐5p and influence the recombinant NELL‐1‐induced osteogenic differentiation of hASCs. It opens a new window to better understanding the effects of NELL‐1 on the osteogenic differentiation of hASCs and provides potential molecular targets and novel methods for bone regeneration efficiently and safely.

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Circular RNA Expression Profile and Analysis of Their Potential Function in Psoriasis

Cited by 59 publications

References 32 publications

Dysregulated circRNA_100876 suppresses proliferation of osteosarcoma cancer cells by targeting microRNA‐136

Dysregulated circRNA_100876 suppresses proliferation of osteosarcoma cancer cells by targeting microRNA‐136

High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome

The roles of circRFWD2 and circINO80 during NELL‐1‐induced osteogenesis

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