circRNA Acbd6 promotes neural stem cell differentiation into cholinergic neurons via the miR-320-5p-Osbpl2 axis

Li, Wen; Shan, Bo-Quan; Cheng, Xiang; He, Hui; Qin, Jianbing; Zhao, Heyan; Tian, Mei; Zhang, Xinhua; Jin, Guohua

doi:10.1016/j.jbc.2022.101828

Cited by 9 publications

(7 citation statements)

References 47 publications

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“…Although circRNA entraps miRNAs thereby preventing their binding to target mRNAs, it remains to be clarified how circRNA decreases the expression levels of target miRNAs ( 60 , 61 ). In this study, we demonstrated that the increased expression of circHIPK3 in RPTECs led to the destabilization of miR-381-3p and thus decreased its expression levels.…”

Section: Discussionmentioning

confidence: 99%

RNA editing enzyme ADAR1 controls miR-381-3p-mediated expression of multidrug resistance protein MRP4 via regulation of circRNA in human renal cells

Omata

Okawa

Haraguchi

et al. 2022

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

RNA editing enzyme ADAR1 controls miR-381-3p-mediated expression of multidrug resistance protein MRP4 via regulation of circRNA in human renal cells

Omata

Okawa

Haraguchi

et al. 2022

Journal of Biological Chemistry

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“…As signal transmitters, exosomes mediate the physiological activities of target cells and promote the repair of TBI. The study suggested that circRNA Acbd6 (containing acyl-CoA binding domain 6) upregulated in exosomes of hippocampal NSCs promotes differentiation of NSCs into cholinergic neurons via the miR-320-5p/oxysterol-binding protein-related protein 2 axis and is targeted to mediate neurogenesis [ 72 ]. In this study, NSC-derived exosomes significantly enhanced the number of newborn neurons (stained for Tuj-1 and ChAT) detected in the hippocampus compared to the controls.…”

Section: Introductionmentioning

confidence: 99%

Neural stem cell-derived exosomes and regeneration: cell-free therapeutic strategies for traumatic brain injury

Zhong

Wang²,

Wang

et al. 2023

Stem Cell Res Ther

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Regenerative repair of the brain after traumatic brain injury (TBI) remains an extensive clinical challenge, inspiring intensified interest in therapeutic approaches to explore superior repair strategies. Exosome therapy is another research hotspot following stem cell alternative therapy. Prior research verified that exosomes produced by neural stem cells can participate in the physiological and pathological changes associated with TBI and have potential neuroregulatory and repair functions. In comparison with their parental stem cells, exosomes have superior stability and immune tolerance and lower tumorigenic risk. In addition, they can readily penetrate the blood‒brain barrier, which makes their treatment efficiency superior to that of transplanted stem cells. Exosomes secreted by neural stem cells present a promising strategy for the development of novel regenerative therapies. Their tissue regeneration and immunomodulatory potential have made them encouraging candidates for TBI repair. The present review addresses the challenges, applications and potential mechanisms of neural stem cell exosomes in regenerating damaged brains.

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“…Accumulating evidence suggests that circRNAs are not only important for neuronal function but also for brain development. Differential expression of circRNAs has been found to occur during neuronal differentiation, both in mice and in cell culture models such as the human neuroblastoma cell line SH-SY5Y, with several specific circRNAs found to have a regulatory role in this process [ 29 , 36 , 61 , 72 , 73 ]. Additionally, a number of circRNAs have been identified as being dysregulated in autism spectrum disorder (ASD) [ 9 , 22 ], while depletion of circRNAs has been observed in individuals with schizophrenia (SCZ) [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Circular RNAs arising from synaptic host genes during human neuronal differentiation are modulated by SFPQ RNA-binding protein

et al. 2023

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Background Circular RNA (circRNA) molecules, generated through non-canonical back-splicing of exon-exon junctions, have recently been implicated in diverse biological functions including transcriptional regulation and modulation of protein interactions. CircRNAs are emerging as a key component of the complex neural transcriptome implicated in brain development. However, the specific expression patterns and functions of circRNAs in human neuronal differentiation have not been explored. Results Using total RNA sequencing analysis, we identified expressed circRNAs during the differentiation of human neuroepithelial stem (NES) cells into developing neurons and discovered that many circRNAs originated from host genes associated with synaptic function. Interestingly, when assessing population data, exons giving rise to circRNAs in our dataset had a higher frequency of genetic variants. Additionally, screening for RNA-binding protein sites identified enrichment of Splicing Factor Proline and Glutamine Rich (SFPQ) motifs in increased circRNAs, several of which were reduced by SFPQ knockdown and enriched in SFPQ ribonucleoprotein complexes. Conclusions Our study provides an in-depth characterisation of circRNAs in a human neuronal differentiation model and highlights SFPQ as both a regulator and binding partner of circRNAs elevated during neuronal maturation.

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circRNA Acbd6 promotes neural stem cell differentiation into cholinergic neurons via the miR-320-5p-Osbpl2 axis

Cited by 9 publications

References 47 publications

RNA editing enzyme ADAR1 controls miR-381-3p-mediated expression of multidrug resistance protein MRP4 via regulation of circRNA in human renal cells

RNA editing enzyme ADAR1 controls miR-381-3p-mediated expression of multidrug resistance protein MRP4 via regulation of circRNA in human renal cells

Neural stem cell-derived exosomes and regeneration: cell-free therapeutic strategies for traumatic brain injury

Circular RNAs arising from synaptic host genes during human neuronal differentiation are modulated by SFPQ RNA-binding protein

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