CircPLK1 sponges miR-296-5p to facilitate triple-negative breast cancer progression

Kong, Yanan; Yang, Lu; Wei-dong, Wang; Lyu, Ning; Zou, Yutian; Gao, Guanfeng; Ou, Xueqi; Xie, Xiaoming

doi:10.2217/epi-2019-0093

Cited by 64 publications

(36 citation statements)

References 39 publications

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“…Circular RNAs have been reported to affect the function of their genes of origin via sponging of miRNAs. 21,27,28 We showed that circ_0060745 was derived from exon 9 to exon 10 of linear CSE1L and that circ_0060745 sequestered miR-4736. Therefore, we evaluated whether CSE1L was a downstream target gene of the circ_0060745/miR-4736 axis.…”

Section: Microrna 4736 Suppressed Proliferation and Metastasis Via Tamentioning

confidence: 91%

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Wang

Ren

et al. 2020

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Purpose: Recent studies have shown that noncoding RNAs (ncRNAs) play essential roles in the development of a number of cancers. Circular RNAs (circRNAs) have been shown to contribute to the progression of colorectal cancer (CRC). Methods: In this study, the expression levels of circular RNA 0060745 (circ_0060745), and microRNA 4736 (miR-4736) were measured using qRT-PCR. Kaplan-Meier survival analysis and receiver operating characteristic (ROC) analysis were used to evaluate the diagnostic value of circ_0060745. Transwell assay and cell counting kit-8 (CCK8) assay were used to determine the metastatic and proliferative capacity of CRC cells. The expression of chromosome segregation one like (CSE1L) was measured using Western blotting and immunohistochemistry (IHC). In addition, RNA pull-down assay and luciferase assay were performed to verify the targeted binding between miR-473,6 and circ_0060745, and between as miR-4736 and CSE1L. Results: We showed that circ_0060745 was upregulated in CRC, and was associated with unfavorable clinicopathological characteristics. We also showed that circ_0060745 acted as an oncogene and promoted CRC cell proliferation and metastasis. Circ_0060745 was primarily located in the cytoplasm. Furthermore, miR-4736 was downregulated in CRC, was a downstream target of circ_0060745, and mediated proliferation and metastasis. We showed that circ_0060745 sequestered miR-4736, which resulted in CRC cell proliferation and metastasis. Finally, we showed that CSE1L, a downstream target of miR-4736, was upregulated in CRC and mediated suppression of proliferation and metastasis in CRC. Conclusion: The results of this study showed that circ_0060745 promoted CRC cell proliferation and metastasis via modulation of miR-4736/CSE1L signaling. The Circ_0060745/miR-4736/CSE1L axis might be a novel target for the treatment of CRC.

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Section: Microrna 4736 Suppressed Proliferation and Metastasis Via Tamentioning

confidence: 91%

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Wang

Ren

et al. 2020

OTT

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“…It was also revealed that circUBAP2 was correlated with tumour size, advanced TNM stage and worse prognosis in TNBC and promoted tumour progression by sponging miR‐661 to upregulate MTA1 136 . Furthermore, circAGFG1, circKIF4A, circPLK1 and circRAD18 were revealed to promote malignant progression of TNBC by sponging corresponding tumour suppressive miRNAs as well 137‐140 . In addition, Zhang et al 141 found that circRNA_069718 can promote the proliferation and invasion of cancer cells by activating the Wnt/β‐catenin pathway, thereby promoting the malignant progression of TNBC.…”

Section: Circular Rnasmentioning

confidence: 98%

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

et al. 2020

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Triple‐negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer with negativity for oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor (HER2). Non‐coding RNAs (ncRNAs) make up most of the transcriptome and are widely present in eukaryotic cells. In recent years, emerging evidence suggests that ncRNAs, mainly microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs), play prominent roles in the tumorigenesis and development of TNBC, but the functions of most ncRNAs have not been fully described. In this review, we systematically elucidate the general characteristics and biogenesis of miRNAs, lncRNAs and circRNAs, discuss the emerging functions of these ncRNAs in TNBC and present future perspectives in clinical practice.

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“…The higher expression levels of circKIF4A correlated with poor OS in TNBC patients (Tang et al, 2019). CircPLK1 was also upregulated in TNBC tissues and correlated with larger tumor size, lymph node positivity, advanced TNM stage and poor OS (Kong Y. et al, 2019). It promotes TNBC metastasis by sponging miR-296-5p and regulating PLK1 expression (Kong Y. et al, 2019).…”

Section: Breast Cancer (Bc)mentioning

confidence: 99%

Circular RNAs: Emerging Role in Cancer Diagnostics and Therapeutics

Rajappa

Banerjee

Sharma

et al. 2020

Front. Mol. Biosci.

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Circular RNAs (circRNAs) are rapidly coming to the fore as major regulators of gene expression and cellular functions. They elicit their influence via a plethora of diverse molecular mechanisms. It is not surprising that aberrant circRNA expression is common in cancers and they have been implicated in multiple aspects of cancer pathophysiology such as apoptosis, invasion, migration, and proliferation. We summarize the emerging role of circRNAs as biomarkers and therapeutic targets in cancer.

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CircPLK1 sponges miR-296-5p to facilitate triple-negative breast cancer progression

Cited by 64 publications

References 39 publications

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Systematic characterization of non‐coding RNAs in triple‐negative breast cancer

Circular RNAs: Emerging Role in Cancer Diagnostics and Therapeutics

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